Plasmid PET24a(+)-SA-hGM-CSF was constructed and maintained in our laboratory. The human erythroleukemia cells (TF-1) and prostate cancer cell line LNCaP as well as PC-3 were purchased from the cell bank of Chinese Academy of Sciences (Beijing, China), and maintained in DMEM/F12 (HyClone，USA). containing 10% (v/v) fetal bovine serum (Gibco，USA), 1% penicillin/streptomycin(Gibco). The mouse bladder cancer cell line (MB49) was purchased from American Type Culture Collection (ATCC), and cultured in RPMI1640 (Gibco) supplemented with 10% fetal bovine serum (Gibco), 1% penicillin/streptomycin(Gibco), at 37°C, in 5% CO2 humidified incubator. Tris (hydroxymethyl) aminomethane, Sodium chloride, Sodium dodecyl sulfate, Ethylenediaminetetraacetic acid, Urea, Glycine, and L-Arginine were obtained from Sangon Biotech Shanghai Co Ltd. The other reagents used in this study were of analytical grade and are commercially available.
L arginine
Manufactured by Sangon
Sourced in China
L-arginine is an amino acid that plays a critical role in various physiological processes. It is an essential component of proteins and participates in the urea cycle, which helps remove excess nitrogen from the body. L-arginine is also involved in the production of nitric oxide, which helps maintain healthy blood flow and circulation. As a lab equipment product, L-arginine can be used in research and scientific applications that require this important amino acid.
Automatically generated - may contain errors
Lab products found in correlation
L histidine hydrochloride, by Sangon (3 mentions)
Penicillin streptomycin solution, by Thermo Fisher Scientific (3 mentions)
Luciferase assay kit, by Promega (3 mentions)
Dithiothreitol (dtt), by Merck Group (3 mentions)
Lipoic acid, by Sangon (2 mentions)
L citrulline, by Sangon (2 mentions)
L proline, by Sangon (2 mentions)
Hydrogen peroxide, by Sangon (2 mentions)
Stearic acid, by Sangon (2 mentions)
L cysteine hydrochloride monohydrate, by Merck Group (2 mentions)
An enhanced bicinchoninic acid protein assay kit, by Beyotime (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Lncap, by National Collection of Authenticated Cell Cultures (1 mentions)
Glycine, by Sangon (1 mentions)
Ethylenediaminetetraacetic acid, by Sangon (1 mentions)
Sodium dodecyl sulfate (sds), by Sangon (1 mentions)
Urea, by Sangon (1 mentions)
Sodium chloride (nacl), by Sangon (1 mentions)
8 protocols using l arginine
1
Construction and Maintenance of Plasmid PET24a(+)-SA-hGM-CSF
2
Lipoic Acid-Functionalized Nanocarriers
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The following materials and reagents used in this study: lipoic acid, L-histidine hydrochloride, and L-arginine (Sangon Biotech, Shanghai, China); 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) (Aladdin, Shanghai, China); DOXO-EMCH (MCE, NJ, USA); dithiothreitol (DTT) (Sigma-Aldrich, St Louis, MO, USA); NH2-PEG-COOH (polyethylene glycol modified y amino and carboxyl) (MW: 2000) (BO Biological Technology Co., Ltd., Jiaxing, China); 4,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich, St. Louis, MO, USA); Cell Counting Kit-8 (Dojindo Molecular Technologies, Tokyo, Japan); Medium RPMI 1640, penicillin streptomycin solution (5 kU/mL), and fetal bovine serum (FBS) (Life Technologies, Grand Island, NY, USA); and CpG (5′-tcgtcgttttcggcgcgcgccg-3′) and Cy-5-labeled CpG (InvivoGen, San Diego, CA, USA).
3
IMQ-Induced Psoriasis Model in Mice
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For the IMQ-induced mouse model of psoriasis, male C57BL/6 mice (7 weeks-of-age) purchased from Shanghai SLAC Laboratory Animal Co. (Shanghai, China) were maintained under SPF conditions. The mice were subjected to a daily topical dose of 62.5 mg IMQ cream (5%) (MedShine cat. 120503) on the shaved back or 20 mg per ear for six consecutive days. On the indicated days, the skin thickness was measured with a micrometer (Schieblehre). The control mice were treated with an equivalent dose of vehicle cream. For the metabolite treatment, L-Arginine (Sangon Biotech cat.
A600205, 5 mg/ml), L-Ornithine (Sigma-Aldrich cat. O6503, 4 mg/ml), L-Proline (Sangon Biotech cat. A600923, 4 mg/ml), Creatine (Sangon Biotech cat. A600326, 10 mg/ml), L-Citrulline (Sangon Biotech cat. A604057, 5 mg/ml) and Spermidine (Sigma-Aldrich cat. S0266, 2 mg/ml) were added to the drinking water 7 days prior to the IMQ application and the treatment lasted until the end of model establishment. application. An equal volume of PBS was injected to the contralateral shoulder of the monkey. The clinical scores of the skin phenotype were graded according to Lattice System Physician's Global Assessment (Langley and Ellis, 2004) .
A600205, 5 mg/ml), L-Ornithine (Sigma-Aldrich cat. O6503, 4 mg/ml), L-Proline (Sangon Biotech cat. A600923, 4 mg/ml), Creatine (Sangon Biotech cat. A600326, 10 mg/ml), L-Citrulline (Sangon Biotech cat. A604057, 5 mg/ml) and Spermidine (Sigma-Aldrich cat. S0266, 2 mg/ml) were added to the drinking water 7 days prior to the IMQ application and the treatment lasted until the end of model establishment. application. An equal volume of PBS was injected to the contralateral shoulder of the monkey. The clinical scores of the skin phenotype were graded according to Lattice System Physician's Global Assessment (Langley and Ellis, 2004) .
4
Optimizing Transfection Efficiency for Gene Delivery
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Materials used in this study were l -histidine hydrochloride, l -arginine, stearic acid, and hydrogen peroxide (30% w/v; Sangon Biotech, Shanghai, People’s Republic of China); polyethylenimine (PEI, branched, molecular weight 25 kDa), Lipofectamine™ 2000, l -buthionine-sulfoximine, 4′,6-diamidino-2-phenylindole dihydrochloride, l -cysteine hydrochloride monohydrate, ethidium bromide, dithiothreitol (DTT, Sigma-Aldrich, St Louis, MO, USA); a luciferase assay kit (Promega, Madison, WI, USA); pDNA (pGL3 and pEGFP) (Shanghai Innovation Biotechnology Co Ltd, Shanghai, People’s Republic of China); enhanced bicinchoninic acid protein assay kit (Beyotime, Nanjing, People’s Republic of China); Dulbecco’s Modified Eagle’s Medium (DMEM), fetal bovine serum, YOYO-1 (Y3601) and penicillin–streptomycin solution 5 kU/mL (Life Technologies, Carlsbad, CA, USA); a Cell Counting Kit-8 (CCK-8, Dojindo Molecular Technologies Inc, Nanjing, People’s Republic of China); and HEK293 cells and HeLa cells (Cell Culture Center of the Shanghai Institutes for Biological Sciences of the Chinese Academy of Sciences, Shanghai, People’s Republic of China). All other reagents were of analytical grade. All animal experiments were performed in accordance with the ethics and regulations of animal experiments of Second Military Medical University (Shanghai, People’s Republic of China).
5
Dialysis Characterization of Biomolecules
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GelNB-loaded molecular weight 3500 Da dialysis bags with deionized water were sealed by clips and immersed in 500 mL of d -Glucose (Meilunbio, China), l -arginine (Sangon Biotech, China), l -glutamic acid (Macklin, China), l -tryptophan (Macklin), glycine (Macklin), l -glutamine (Macklin), l -cysteine (Macklin), or Ringer's solution, respectively. As a control, deionized water was placed in a separate clean dialysis bag. The liquid in the dialysis bags was collected in a vacuum tube at regular intervals as a dialysis test sample. The amino acid concentrations were determined using a total amino acid assay kit (Nanjing Jiancheng Bio-engineering Institute, China). The glucose concentration was assessed using a glucose colorimetric assay kit (Elabscience, China). The ion concentrations were measured using an automatic biochemical analyzer (Beckman Coulter AU5800, USA).
6
Luminescent Nanoparticle-based Prostate Cancer Assay
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Enz, aminated GQDs, MAL-PEG-SCM (PEG molecular size: 2000), l -arginine, stearic acid, and hydrogen peroxide (30% w/v) were purchased from Sangon Biotech, Shanghai, China. Dithiothreitol (DTT) was purchased from Sigma-Aldrich (St Louis, MO, USA). A luciferase assay kit was gifted from Promega (Madison, WI, USA). An enhanced bicinchoninic acid protein assay kit was purchased from Beyotime (Nanjing, China). Dulbecco’s Modified Eagle Medium (DMEM), RPMI-1640 medium, foetal bovine serum (FBS), penicillin–streptomycin solution (5 KU/mL) was obtained from Life Technologies (Carlsbad, CA, USA). Cell Counting Kit-8 (CCK-8) was purchased from Dojindo Molecular Technologies Inc., Nanjing, China. LNCaP and C4-2B cells were obtained from Shanghai Cell Bank, Chinese Academy of Sciences, Shanghai, China. All other reagents were analytical grade. All animals were supplied from Department of Pharmacy of Fudan University and all animal experiments were performed in accordance with the ethics and regulations of animal experiments at Fudan University (Shanghai, China).
7
Transfection and Cytotoxicity Evaluation
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L-histidine hydrochloride, L-arginine and lipoic acid were purchased from Sangon Biotech (Shanghai, China). L-cysteine hydrochloride monohydrate and dithiothreitol (DTT) were purchased from Sigma-Aldrich (St Louis, MO, USA). A luciferase assay kit was gifted from Promega (Madison, WI, USA). An enhanced bicinchoninic acid protein assay kit was purchased from Beyotime (Nanjing, China). Dulbecco's Modified Eagle' Medium (DMEM), fetal bovine serum (FBS), YOYO-(Y3601) and a penicillin-streptomycin solution (5 KU/mL) were purchased from Life Technologies (Carlsbad, CA, USA). The Cell Counting Kit-8 (CCK-8) was purchased from Dojindo Molecular Technologies, Inc. (Nanjing, China). Propidium iodide (PI) and an apoptosis kit were purchased from Invitrogen (Oregon, USA). pDNA (pGL3, pEGFP and pTRAIL) was purchased from Shanghai Innovation Biotechnology Co., Ltd (Shanghai, China). Doxorubicin (DOX) hydrochloride was gifted from Hisun Pharmaceutical Co., Ltd (Zhejing, China).
8
Ferroptosis and Cell Death Modulators
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The following compounds were obtained from TargetMol (USA): RSL3 (T3646), IKE (T5523), ferrostatin-1 (T6500), deferoxamine mesylate (T1637), Z-VAD (OMe)-FMK (T6013), necrosulfonamide (T7129), FIN56 (T4066), FINO2 (T60084), LF3 (T7399), SKL2001 (T6989), cisplatin (T1564), aminoguanidine hydrochloride (T0358). The following compounds were obtained from MedChemExpress (MCE, USA): AMXT-1501 (HY-124617A), DFMO (HY-B0744), GC7 (HY-108314A). The following compounds were obtained from Sangon Biotech (China): l -arginine (A600205), l -citrulline (A604057L), l -proline (A600923), putrescine dihydrochloride (A600793). The following compounds were obtained from Aladdin (China): L-ornithine monohydrochloride (O108803), urea (A610148), spermidine (S107071), spermine (S109704), ammonium ferric citrate (A100170).
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