Talin 1

Manufactured by Merck Group

Talin-1 is a laboratory equipment product offered by Merck Group. It is a protein that plays a crucial role in the regulation of cell adhesion and migration. Talin-1 acts as a molecular link between the extracellular matrix and the actin cytoskeleton, facilitating the formation and stabilization of focal adhesions.

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Lab products found in correlation

Lamin b1, by Santa Cruz Biotechnology (2 mentions) Nitrocellulose, by Bio-Rad (1 mentions) Horseradish peroxidase labeled anti mouse igg, by Bio-Rad (1 mentions) P erk1 2 thr202 tyr204, by Cell Signaling Technology (1 mentions) Anti rabbit igg alexa fluor 488 conjugate, by Cell Signaling Technology (1 mentions) Mouse monoclonal β actin antibody, by Cell Signaling Technology (1 mentions) Anti mouse igg alexa fluor 594 conjugate, by Cell Signaling Technology (1 mentions) Ha tag antibody, by Cell Signaling Technology (1 mentions) Bckdk e 12, by Santa Cruz Biotechnology (1 mentions) Pakt s473, by Cell Signaling Technology (1 mentions) Erk1 2, by Cell Signaling Technology (1 mentions) Ab37154, by Abcam (1 mentions) Myd88, by Santa Cruz Biotechnology (1 mentions)

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Talin

3 protocols using talin 1

Immunoblotting of T Cell Lysates

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Total lymphocytes or cell-sorted naive CD4⁺ T cell lysates (15 μg) were resolved by 5% SDS-PAGE and transferred to nitrocellulose (BioRad). Membranes were probed with primary antibodies against Talin-1 (Sigma-Aldrich) or Lamin B1 (Santa Cruz) overnight at 4°C. Signals were detected using a horseradish peroxidase–labeled anti-mouse IgG (BioRad) and enhanced chemiluminescence (ECL; PerkinElmer) on HyBlot CL film (Harvard Apparatus).

Meli A.P., Fontés G., Avery D.T., Leddon S.A., Tam M., Elliot M., Ballesteros-Tato A., Miller J., Stevenson M.M., Fowell D.J., Tangye S.G, & King I.L. (2016). The Integrin LFA-1 Controls T Follicular Helper Cell Generation and Maintenance. Immunity, 45(4), 831-846.

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Antibody Use for Cell Signaling

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The BCKDK (E-12) (1:1000, sc-374425) antibodies were purchased from Santa Cruz Technology. Flag tag and talin-1 (#4021) antibodies were purchased from Sigma-Aldrich. All the following antibodies were purchased from Cell Signaling Technology: ERK1/2 (#4695), p-ERK1/2 (Thr202/Tyr204) (#4649), MEK1/2 (8727s), p-MEK1/2 (S221) (2338s), β1-integrin (D6S1W), TRIM21 (#92043), AKT (#4060), p-AKT (S473) (#13038), HA tag antibody (#3724), β-actin mouse monoclonal antibody (#3700), Anti-rabbit IgG (Alexa Fluor#488 Conjugate) (4412), Anti-mouse IgG (Alexa Fluor#594 Conjugate) (8890), secondary antibody against mouse and rabbit. The FAK (A11131), pY397-FAK (AP0302), talin-2 (A19810), and BCAT2 (A7426) antibodies were purchased from ABclonal. The vinculin (ab129002), BCKDHA (ab305168), p-BCKDHA (S293) (ab302504) antibodies were purchased from Abcam. All antibodies were used following the manufacturer’s instructions.

Xu C., Yang K., Xuan Z., Li J., Liu Y., Zhao Y., Zheng Z., Bai Y., Shi Z., Shao C., Zhang L, & Sun H. (2023). BCKDK regulates breast cancer cell adhesion and tumor metastasis by inhibiting TRIM21 ubiquitinate talin1. Cell Death & Disease, 14(7), 445.

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Western Blotting Analysis of Immune Signaling

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Western blotting (immunoblotting) was performed according to standard protocols. The following antibodies were used: TLR3 (6961), TLR4 (14358), MyD88 (4283), TIRAP (13077), TRIF (4596), IRAK4 (4364), TRAF3 (4729), Vav1 (4657), Vav1 (2502), p85 (4257), PIP5K1c (3296), p85 (4292), pERK1/2 T202/Y204 (4370), ERK1/2 (4695), pAKT S473 (4060), pAKT T308 (13038), pJNK1/2 T183/Y185 (9255), pp38 T180/Y182 (9211), pIRF3 S396 (4947), pIκBα S32 (2859), FAK (3285), EEA-1 (3288), pTBK1 S172 (5483), TBK1 (3504; all from CST); CD11b M1/70 (ab184308), CD29 (ab183666), TLR9 (ab37154; all from Abcam); TLR4 (sc-293072), TLR3 (sc-32232), GFP (sc-9996), GFP (sc-8334), Vav (sc-132), MyD88 (sc-74532), MyD88 (sc-136970), Na/K ATPase (sc-21712), AKT (sc-1618), JNK1/2 (sc-7345), p38 (sc-7972), IRF3 (sc-376455), lamin B1 (sc-374015), p65 (sc-109), CD29 (sc-8978), IκBα (sc-1643; all from Santa Cruz Biotechnology); TRAM (AF4348; R&D Biosystems), TRAF6 (GTX113029; GeneTex), Talin1 (T3287), Flag M2 (F3165 and F7425; both from Sigma-Aldrich); GAPDH (AM4300), IRAK4 (700026), and TLR4 (48–2300; all from Invitrogen).

Lim T.J., Bunjamin M., Ruedl C, & Su I.H. (2020). Talin1 controls dendritic cell activation by regulating TLR complex assembly and signaling. The Journal of Experimental Medicine, 217(8), e20191810.

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