Vimentin

Manufactured by SCBT

Sourced in United States

Vimentin is a type III intermediate filament protein that is widely expressed in various cell types, particularly in cells of mesenchymal origin. Vimentin plays a key structural role in maintaining the integrity and shape of cells.

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Lab products found in correlation

Cycloheximide (chx), by Merck Group (1 mentions) Puromycin, by Merck Group (1 mentions) Rapamycin, by Enzo Life Sciences (1 mentions) Becn1, by Cell Signaling Technology (1 mentions) Phospho mtor, by Abcam (1 mentions) Pik3c3, by Cell Signaling Technology (1 mentions) Poly ubiquitin, by Agilent Technologies (1 mentions) Wipi1, by Merck Group (1 mentions) Bafilomycin a1, by LC Laboratories (1 mentions) Streptavidin biotin peroxidase kit, by ZSGB-BIO (1 mentions) Dab staining, by ZSGB-BIO (1 mentions)

2 protocols using vimentin

Autophagy-related Protein Detection

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Stock solutions of Bafilomycin A1 (LC Laboratories, B-1080), MG132 (Calbiochem, 474790), Cycloheximide (Sigma, 01810) and Rapamycin (Enzo, BML-A275-0025) were prepared in DMSO (Roth, A994.2). Stock solution of Canavanine (Santa Cruz Biotech, sc-202983A) and Puromycin (Sigma, P8833) was prepared in distilled H2O.
Antibody sources were as follows: for Actin (Sigma, A5060), BAG1 (Abcam, ab7976), BAG3 (Proteintech Group, 10599-1-AP), BECN1 (Cell Signaling, 3495), CTSD (Abcam, ab75852), DLP1 (BD Transduction Laboratories, 611113), LAMP2 (DSHB Biology, ABL-93), LC3B (Nanotools, 0260-100), LC3B (Sigma, L7543), OPA1 (BD Transduction Laboratories, 612607), Phospho mTOR (Abcam, ab109268), Puromycin (Millipore, MABE343), mTOR (Calbiochem, OP97), p62 (Progen, GP62-C), PIK3C3 (Cell Signaling, 4263), Poly-Ubiquitin (Dako, Z0458), RAB18 (Sigma, SAB4200173), Tubulin (Millipore, MAB1637), Tubulin (Sigma, T9026), TFEB (Proteintech Group, 13372-1-AP), Vimentin (SCBT, sc-373717), WIPI1 (Sigma, HPA007493).

Chakraborty D., Felzen V., Hiebel C., Stürner E., Perumal N., Manicam C., Sehn E., Grus F., Wolfrum U, & Behl C. (2019). Enhanced autophagic-lysosomal activity and increased BAG3-mediated selective macroautophagy as adaptive response of neuronal cells to chronic oxidative stress. Redox Biology, 24, 101181.

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Immunohistochemical Analysis of E-cadherin, Vimentin, and HBx

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Immunohistochemistry was performed on the tissue microarray slide conventionally. In brief, the slide was deparaffinized by xylene and rehydrated using a graded ethanol series. Then, 3% H₂O₂ was used to block endogenous peroxidase in the tissues. Antigen retrieval was completed using microwave treatment. Then, 5% bovine serum albumin was used to block nonspecific reactions.The slides were incubated with primary antibody against E-cadherin (SCBT, Santa Cruz, CA, USA, 1:100), vimentin (SCBT, 1:100) and HBx (US Biological, Swampscott, MA, USA, 1:100) at 4 °C overnight. The streptavidin-biotin peroxidase kit (ZSGB Bio, Beijing, China) was used to detect the bound antibodies. Finally, the band was visualized by DAB staining (ZSGB Bio).
The immunohistochemical result was scored using the intensity and extent. Staining intensity was quantified as follows: negative (0), weak (1), moderate (2) or strong (3). Staining extent was quantified according to the percentage of positive cells: none (0), <25% (1), 25–50% (2), 50–75% (3) or >75% (4). The final score was calculated as the intensity score multiplied by the extent score.

Jin Y., Wu D., Yang W., Weng M., Li Y., Wang X., Zhang X., Jin X, & Wang T. (2017). Hepatitis B virus x protein induces epithelial-mesenchymal transition of hepatocellular carcinoma cells by regulating long non-coding RNA. Virology Journal, 14, 238.

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