Mouse anti flag

Both protease and phosphatase inhibitors were purchased from Roche (Basel, Switzerland). Primary antibodies used in western blotting are as follows: Mouse anti-β-actin (1:5000, Proteintech), Rabbit anti-p-α-syn (1:1000, Abcam), Mouse anti-p-α-syn (1:1000, Wako), Mouse anti-α-syn (1:1000, Santa Cruz), Rabbit anti-Human α-syn (1:2000, Abcam), Mouse anti-demethylate-PP2A (1:1000, Millipore), Mouse anti-PP2A (1:1000, BD biosciences), Mouse anti-β-tubulin (1:1000, Abcam), Mouse anti-PPP2R2A (1:1000, CST), Mouse anti-PPP2R2B (1:1000, Abcam), Rabbit anti-PPP2R2D (1:1000, Abcam), Mouse anti-PPP2R5A (1:500, Santa Cruz), Rabbit anti-PPP2R5D (1:5000, Abcam), Rabbit anti-PPP2R5E (1:1000, Abcam), Mouse anti-LCMT-1 (1:1000, Abcam), Rabbit anti-PME-1 (1:1000, Millipore), Mouse anti-GAPDH (1:3000, Sigma), Mouse anti-c-Myc (1:5000, Clontech), and Mouse anti-FLAG (1:500, Santa Cruz). Secondary antibodies are as follows: Goat anti-Rabbit IRdye680 (LI-COR Bioscience), Goat anti-Mouse IRdye680 (LI-COR Bioscience), Goat anti-Rabbit IRdye800 (LI-COR Bioscience), Goat anti-Mouse IRdye800 (LI-COR Bioscience), Goat anti-Rabbit Alexa Fluor 488 (Thermo Fisher Scientific). The inhibitor of protein phosphatase methylesterase-1 (PME-1), AMZ30, was purchased from MedChem Express (Shanghai, China). Dulbecco’s Modified Eagle’s Medium (DMEM), and fetal bovine serum (FBS) were purchased from Gibco.

Rabbit anti-CLDN1, mouse anti-CLDN4, and rabbit anti-ZO-1 antibodies were obtained from Thermo Fisher Scientific (San Diego, CA, USA). Goat anti-β-actin and mouse anti-FLAG antibodies were from Santa Cruz Biotechnology (Santa Cruz, CA, USA) and Wako Pure Chemical (Osaka, Japan), respectively. Mouse anti-p-Ser and anti-p-Thr antibodies were from Sigma-Aldrich (Saint Louis, MO, USA). EBGP, LY, H₂DCFDA, and PMA were from Yamada Bee Company, Inc. (Lot: LY-009, Okayama, Japan), Biotium (Fremont, CA, USA), Thermo Fisher Scientific, and LC Laboratories (Woburn, MA, USA), respectively. OxiOrange and Hydrop were from Goryo Chemical (Hokkaido, Japan). All other reagents were of the highest grade of purity available.