Peripheral venous blood samples (4 ml) were collected from all the participants after an overnight fast of 12–14 h. Serum was separated by centrifuging at 3000 rpm for 5 min. Blood glucose was estimated by hexokinase method on Olympus AU2700 analyzer. Blood urea was estimated by enzymatic urease method and serum creatinine by Jaffe's method. Serum calcium was estimated by the Arsenazo III method. Liver function was estimated by the colorimetry method on Olympus AU2700 analyzer. High-density lipoprotein (HDL), LDL, very low density lipoprotein (VLDL), and triglycerides were estimated by an enzymatic method on Olympus AU2700 analyzer.
Au2700 analyzer
Manufactured by Olympus
Sourced in Japan, Ireland
The AU2700 analyzer is a clinical chemistry analyzer designed for in-vitro diagnostic testing. It is capable of performing a variety of laboratory tests, including those for clinical chemistry, immunoassay, and urinalysis. The analyzer utilizes advanced technology and automation to provide accurate and reliable results.
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Lab products found in correlation
31 protocols using au2700 analyzer
1
Biochemical Profiling of Fasted Participants
2
Comprehensive Blood Profiling Protocol
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Pre-exposure and post-exposure venous blood samples were collected from the participants by qualified nurses using EDTA-coated tubes and standard procedures. The blood samples were stored at 4°C until the testing using the AU-2700 analyzers (Olympus, Tokyo, Japan) and commercial reagents. The hematological parameters were white blood cell count (WBC), red blood cell count (RBC), hematocrit (HCT), mean corpuscular volume, red cell distribution width and its coefficient of variation, platelet count, mean platelet volume, plateletcrit, platelet distribution width, platelet-large cell ratio, hemoglobin (HGB), mean corpuscular hemoglobin and concentration, neutrophil count (NEU#), reticulocyte count (RET#), lymphocyte count (LYM#), eosinophil count (EOS#), monocyte count (MON#), neutrophil percentage (NEU%), reticulocyte percentage (RET%), lymphocyte percentage (LYM%), eosinophil percentage (EOS%), and monocyte percentage (MON%) (Supplementary Table 1 ).
3
Saliva and Blood Collection Protocol
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At baseline, up to 5 mL of saliva sample was obtained in a 50-mL centrifuge tube from each individual. In order to stimulate glandular salivary flow, the cotton swab with 2% citric acid solution was provided, which is used to touch the bilateral posterior lateral surfaces of the tongue (5 s every 30 s) (Xie et al., 2013 (link)). Then, a total of 2 mL of saliva was removed from the tube as whole saliva sample. The remaining 3 mL of saliva sample was centrifuged at a speed of 3,000 × g for 15 min under 4°C, to spin down the exfoliated cells. After that, the supernatant was further centrifuged (12,000 × g, 10 min, 4°C) to completely remove the cellular components. Finally, the samples (whole saliva and supernatant saliva) were aliquoted into RNase/DNase free Eppendorf tubes and stored at −80°C until assay. Based on the previous methods of Xie et al. (2013 (link), 2015) (link), using citric acid in cotton swab can stimulate glandular salivary flow, but does not alter the results. At baseline and after the exposure at 4,500 m, venous blood samples were collected from the participants by qualified nurses using EDTA-coated tubes and standard procedures. The blood samples were stored at 4°C until further testing. Then, the white blood cell count (WBC) was analyzed using the AU-2700 analyzers (Olympus, Tokyo, Japan) and commercial reagents.
4
Urethane Anesthesia and Serum Analysis
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All of the rats were anesthetized with 30% urethane (1,400 mg/kg, intraperitoneal) at 24 h after ROSC and transcardially perfused with 4% paraformaldehyde and 5 ml of blood was collected from the inferior vena cava. The blood was centrifuged at 1,413 x g for 15 min at 4˚C and serum was obtained for the determination of BUN and Cr with an Olympus AU 2700 Analyzer (Olympus Optical Co., Ltd.).
5
Kidney Tissue and Blood Analysis
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After the 24-h reperfusion period, blood samples were collected from the inferior vena cava and centrifuged to determine the concentration of creatinine (Cr). The Cr was measured in the blood using standard techniques with an Olympus AU 2700 Analyzer (Olympus Optical Co., Ltd., Tokyo, Japan).
After a 24 h reperfusion, the left kidney was excised. Then, the kidney tissue was fixed with 10% phosphate-buffered formalin, paraffin embedded, and sectioned to a thickness of 4 mm according to a standard procedure. Sections were deparaffinized and gradually hydrated before they were examined by hematoxylin and eosin (HE) staining. Morphological assessments were performed by an experienced renal pathologist who had not been informed of the experimental protocol.
After a 24 h reperfusion, the left kidney was excised. Then, the kidney tissue was fixed with 10% phosphate-buffered formalin, paraffin embedded, and sectioned to a thickness of 4 mm according to a standard procedure. Sections were deparaffinized and gradually hydrated before they were examined by hematoxylin and eosin (HE) staining. Morphological assessments were performed by an experienced renal pathologist who had not been informed of the experimental protocol.
6
Serum Biomarker Determination
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Serum levels of creatinine, urea, alanine aminotransferase (ALAT) and aspartate aminotransferase (ASAT) were determined by standard methods on an Olympus AU 2700 Analyzer (Olympus Optical Co., Ltd., Tokyo, Japan). All determinations were performed blindly with respect to group allocation or treatment.
7
Serum BUN and Creatinine Analysis
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The serum was obtained from the blood in inferior vena cava by centrifugation at 4000× g for 15 min. Serum BUN and creatinine levels were determined by Olympus AU 2700 Analyzer (Olympus Optical Co., Ltd., Tokyo, Japan).
8
Biochemical Markers of Kidney Function
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Serum creatinine (Cr), urea nitrogen (BUN) and electrolyte including potassium (K), inorganic phosphorus (IP) and total calcium (Ca) were measured by standard techniques using an Olympus AU 2700 Analyzer (Olympus Optical Co, Ltd, Tokyo, Japan).
Kidney tissue was sequentially rinsed with PBS (0.01 M, pH=7.4), weighted and homogenized in PBS. Then the homogenate was centrifuged at 5000 rpm for 10 min at 4 °C. After that, the supernatant was collected to detect SOD, CAT, GSH, MDA, PCO concentration by using corresponding commercial assay kits (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) according to the manufacturer instruction. Absorbance of SOD, CAT and GSH was respectively measured at 450 nm, 405 nm and 450 nm, while absorbance of MDA and PCO was respectively determined at 532 nm and 370 nm by using a spectrometer. Results were normalized to the total protein concentrations of renal tissue homogenate samples measured with Coomassie blue method by using assay kits (Nanjing Jiancheng Bioengineering Institute, Nanjing, China).
Kidney tissue was sequentially rinsed with PBS (0.01 M, pH=7.4), weighted and homogenized in PBS. Then the homogenate was centrifuged at 5000 rpm for 10 min at 4 °C. After that, the supernatant was collected to detect SOD, CAT, GSH, MDA, PCO concentration by using corresponding commercial assay kits (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) according to the manufacturer instruction. Absorbance of SOD, CAT and GSH was respectively measured at 450 nm, 405 nm and 450 nm, while absorbance of MDA and PCO was respectively determined at 532 nm and 370 nm by using a spectrometer. Results were normalized to the total protein concentrations of renal tissue homogenate samples measured with Coomassie blue method by using assay kits (Nanjing Jiancheng Bioengineering Institute, Nanjing, China).
9
Assessing Blood Cr and BUN Levels
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To assess Cr and BUN, blood samples (n=6 for each group) were collected, centrifuged and kept at -20°C until analyses, adopting standard techniques using an Olympus AU 2700 Analyzer (Olympus Optical Co. Ltd, Tokyo, Japan).
10
Evaluation of Kidney Function
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After a 24-h reperfusion, all the rats were euthanized and 5-mL blood samples were collected from the inferior vena cava, and centrifuged for the determination of concentration of blood urea nitrogen (BUN) and creatinine (Cr). These parameters were measured in the blood using standard techniques with an Olympus AU 2700 Analyzer (Olympus Optical Co., Ltd., Tokyo, Japan).
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