Stat1

The siRNAs of JAK1, JAK2, STAT1 and IRF1 were purchased from Shanghai GenePharma (Shanghai, China). Lipofectamine® 3000 Reagent (Invitrogen, Carlsbad, USA) was used to transfect with SiRNA according to the manufacturer's protocol. The siRNA sequences of all target genes were listed in Table 1.

STAT1 overexpression vector was obtained by cloning the sequences of STAT1 (Shanghai GenePharma Co., Ltd.) into pcDNA3.1 (Invitrogen; Thermo Fisher Scientific, Inc.) and termed pcDNA3.1-STAT1 (STAT1). Before transfection, THP-1 cells (4x10⁵ cells per well) were seeded into 12-well plate. After incubation for 24 h, 0.2 µg of STAT1 overexpression vector (STAT1) and the control pcDNA were transfected into THP-1 cells using 0.5 µl of Lipofectamine 3000 reagent (Invitrogen; Thermo Fisher Scientific, Inc.). Furthermore, the oligonucleotide sequences were as follows: miR-150 mimic (5'-UCUCCCAACCCUUGUACCAGUG-3') and negative control (miR-NC: 5'-GCCUCCGUACCGAUCCUACUUA-3'); miR-150 inhibitor (5'-CACUGGUACAAGGGUUGGGAGA-3') and the negative control (anti-miR-NC: 5'-GGACAGGAUGGUCGAAACUGGU-3'); and small interfering RNA for STAT1 (si-STAT1: 5'-CCGGCTGGAAGATTTACAAGATGAACTCGAGTTCATCTTGTAAATCTTCCAGTTTTTG-3') and the negative control (si-NC: 5'-CCGGTTCTCCGAACGTGTCACGTTTCAAGAGAACGTGACACGTTCGGAGAATTTTTG-3'). These were purchased from Guangzhou RiboBio Co., Ltd. THP-1 cells were transfected with 0.5 µg of the aforementioned oligonucleotides using 0.6 µl of Lipofectamine 3000 (Invitrogen; Thermo Fisher Scientific, Inc.). After 24 h, THP-1 cells treated with 1 µg/ml LPS and untreated cells were incubated for a further 24 h at 37˚C prior to further experimentation.