Inveon pet spect ct scanner

Manufactured by Siemens

Sourced in United States

The Inveon PET/SPECT/CT scanner is a multimodal imaging system that integrates positron emission tomography (PET), single-photon emission computed tomography (SPECT), and computed tomography (CT) technologies. It is designed to acquire high-resolution images for preclinical research applications.

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8 protocols using inveon pet spect ct scanner

Quantitative Scaffold Microstructure Analysis

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MicroCT was performed using a Siemens Inveon PET/SPECT/CT scanner (Siemens Medical Solutions, Malvern, PA, USA). The cone-beam CT parameters were as follows: 360° rotation, 720 projections, 1300 ms exposure time, 1500 ms settle time, 80 kV voltage, 500 μA current, and effective pixel size 17.67 μm. Acquisitions were reconstructed using a Feldkamp algorithm with Shepp-Logan filter and beam-hardening correction, matrix size 1024 × 1472 × 721, using manufacturer-provided software. Protocol-specific Hounsfield Unit (HU) calibration factor was applied. Quality control included HU constancy using rat femur phantom standards with the same protocol.
3D scaffold segmentation was performed using ROI thresholding tools of the Siemens Research Workplace software (version 4.2) to calculate volumes of the scaffold structures and deposits. The number of all pixels which contained scaffold material were counted, ensuring voids were not included in the count by setting a threshold of HU > −850. Then a threshold of HU values > 0, was set to count pixels for deposits in the scaffold. A third threshold was applied to count extremely dense deposits with HU values > 1000. Percent volumes (# pixels in deposits/# pixels in scaffold) were then calculated for each.

Murab S., Gruber S.M., Lin C.Y, & Whitlock P. (2019). Elucidation of bio-inspired hydroxyapatie crystallization on oxygen-plasma modified 3D printed poly-caprolactone scaffolds. Materials science & engineering. C, Materials for biological applications, 109, 110529.

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Quantifying Tumor Hypoxia via [18F]-FMISO PET

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Tumor-bearing mice were kept under anesthesia with a mixture of isoflurane (2%) and O₂ (2 L/min) continuously administered via a nose cone during the entire experiment. During the whole timeframe of the experiment, animals were kept at a body temperature of 37 °C and physiological parameter such as breathing frequency was monitored. Anesthetized animals were positioned in the imaging chamber of the small animal Inveon PET/SPECT-CT scanner (Siemens Medical Solutions, Knoxville, TN). The radiotracer [¹⁸F]FMISO (10–15 MBq) was injected via the lateral tail vein and the acquisition of static microPET images was performed 2 h post-injection over 10 min. Additionally, each animal was imaged for 10 min using high-resolution small-animal cone-beam CT (Inveon PET/SPECT/CT), which was co-registered to the PET image and used as anatomical landmarks to draw 3D VOIs. In hypoxic tissue, [¹⁸F]-FMISO is reduced, bound covalently to intracellular macromolecules, and quantifies the amount of hypoxia in cancer cells (compare Table 1).

Zopf L.M., Heimel P., Geyer S.H., Kavirayani A., Reier S., Fröhlich V., Stiglbauer-Tscholakoff A., Chen Z., Nics L., Zinnanti J., Drexler W., Mitterhauser M., Helbich T., Weninger W.J., Slezak P., Obenauf A., Bühler K, & Walter A. (2021). Cross-Modality Imaging of Murine Tumor Vasculature—a Feasibility Study. Molecular Imaging and Biology, 23(6), 874-893.

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Multimodal Imaging of Small Animals

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All images were acquired using Siemens Inveon PET/SPECT/CT scanner with software version 2.0. Acquisition parameters were as follows: CT: 120 projections were collected in 220 degrees of scan arc. The X-ray exposure time was 0.13 sec per projection and the X-ray tube settings were 80 kVp and 500 μA. The resulting raw data were reconstructed to a final image matrix of 480 × 480 × 667 slices at 196 μm voxel size. PET: thirty minute scans were performed and the 3D OSEM-MAP algorithm (two OSEM and 18 MAP iterations) was used to reconstruct the sinograms into a 128 × 128 × 159 matrix at 0.78 × 0.78 × 0.79 mm³ voxel size. CT-based attenuation compensation was performed. All mice underwent PET imaging at four or more time points.

Kothari P., De B.P., He B., Chen A., Chiuchiolo M.J., Kim D., Nikolopoulou A., Amor-Coarasa A., Dyke J.P., Voss H.U., Kaminsky S.M., Foley C.P., Vallabhajosula S., Hu B., DiMagno S.G., Sondhi D., Crystal R.G., Babich J.W, & Ballon D. (2017). Radioiodinated Capsids Facilitate In Vivo Non-Invasive Tracking of Adeno-Associated Gene Transfer Vectors. Scientific Reports, 7, 39594.

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High-Resolution 3D Imaging of Biological Samples

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MicroCT was performed using a Siemens Inveon PET/SPECT/CT scanner (Siemens Medical Solutions, Malvern, PA, USA). The cone-beam CT parameters were as follows: 360° rotation, 1080 projections, 1300 ms exposure time, 1500 ms settle time, 80 kVp voltage, 500 μA current, and effective pixel size 17.67 μm. Acquisitions were reconstructed using a Feldkamp algorithm with mouse beam-hardening correction, slight noise reduction, 3D matrix size 1024×1024×1536, using manufacturer-provided software. Protocol-specific Hounsfield Unit (HU) calibration factor was applied.

Ho B.L., Goh Q., Nikolaou S., Hu L., Shay‐Winkler K, & Cornwall R. (2021). NRG/ErbB signaling regulates neonatal muscle growth but not neuromuscular contractures in neonatal brachial plexus injury. Febs Letters, 595(5), 655-666.

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Micro-CT Imaging of Small Samples

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MicroCT was performed using a Siemens Inveon PET/SPECT/CT Scanner (Siemens Medical Solutions, Malvern, PA, USA) as previously described (Ho et al., 2021 (link)). The cone-beam CT parameters were as follows: 360° rotation, 1080 projections, 1300-ms exposure time, 1500-ms settle time, 80-kVp voltage, 500-µA current, and effective pixel size 17.67 µm. Briefly, acquisitions were reconstructed using a Feldkamp algorithm with mouse beam-hardening correction, slight noise reduction, and 3D matrix size 1024 × 1024 × 1536, using manufacturer-provided software. Protocol-specific Hounsfield unit (HU) calibration factor was applied.

Emmert M.E., Aggarwal P., Shay-Winkler K., Lee S.J., Goh Q, & Cornwall R. (2022). Sex-specific role of myostatin signaling in neonatal muscle growth, denervation atrophy, and neuromuscular contractures. eLife, 11, e81121.

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Micro-CT Imaging Protocol for Murine Studies

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micro‐CT was performed using a Siemens Inveon PET/SPECT/CT Scanner (Siemens Medical Solutions, Malvern, PA, USA). The cone‐beam CT parameters were as follows: 360° rotation, 1080 projections, 1300‐ms exposure time, 1500‐ms settle time, 80‐kVp voltage, 500‐μA current, and effective pixel size 17.67 μm. Acquisitions were reconstructed using a Feldkamp algorithm with mouse beam‐hardening correction, slight noise reduction, and 3D matrix size 1024x1024x1536, using manufacturer‐provided software. Protocol‐specific Hounsfield unit (HU) calibration factor was applied.

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Quantifying Trabecular Bone Volume in Rodents

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For gross analysis of trabecular bone volume, formalin fixed tibiae were scanned at an isotropic voxel size of 18μm (Siemens Inveon PET/CT/SPECT scanner with cone beam X-Ray source). The scans were performed at 80kVp voltage and 500 mA current with an exposition time of 3000 ms per projection. The tissue volume (TV) was derived from generating a contour around the metaphyseal trabecular bone that excluded the cortices. The area of measurement began at least 0.5mm below the growth plate and was extended by 1.8mm (100×8μm slices). The bone volume (BV) included all bone tissue that had a material density greater than 438.7 mg HA/cm³. These analyses allowed for the calculation of the BV/TV ratio. The threshold setting for bone tissue was determined based on control sham tibia and was used for all samples. Radiographic images (Faxitron X-ray Corp) were obtained using an energy of 35kVp and an exposure time of 8 ms. The spatial resolution is 10 lp/mm (48μm). The tumor volume (TuV) was calculated as a function of the total tissue volume (TV) of the tibial medullary canal using ImageJ software (26 (link)).

Tauro M., Shay G., Sansil S.S., Laghezza A., Tortorella P., Neuger AM A.M., Soliman H, & Lynch C.C. (2017). Bone seeking matrix metalloproteinase-2 inhibitors prevent bone metastatic breast cancer growth. Molecular cancer therapeutics, 16(3), 494-505.

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Lung Fixation and Micro-CT Analysis

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Mice were euthanized with an intraperitoneal injection (Euthasol; Vibrac, Fort Worth, TX), and their lungs were surgically excised. The lungs were fixed via airway instillation at a pressure of 25 cmH₂O as previously described [64 (link)]. The fixative was composed (by volume) of 55% distilled water, 25% polyethylene glycol, 10% ethyl alcohol (190 proof), and 10% formaldehyde. The lungs were removed from the fixative after 24 hr and placed in an oven at 60°C for 24 hr. While in the oven, an airway pressure of 25 cmH₂O was maintained.
Lungs were imaged by micro computed tomography (micro-CT) on a Siemen’s Inveon PET/CT/SPECT scanner. Scanner settings were: 50 kVp voltage, 500 μA current, 2150 ms exposure time, 360 degrees of rotation, and 720 projections. The resulting voxels were cuboidal with 40 μm sides. Airway measurements were obtained from the micro-CT scans with Pulmonary Workstation 2.0 (VIDA Diagnostics Inc., Coralville, IA) as previously described [64 (link)]. Measurements were made perpendicular to the airway centerline and were obtained for the 35 airways highlighted by Thiesse et al. [65 (link)]. Airway measurements occurred over a period of several days, with the operator blinded to genotype.

Reznikov L.R., Meyerholz D.K., Adam R.J., Abou Alaiwa M., Jaffer O., Michalski A.S., Powers L.S., Price M.P., Stoltz D.A, & Welsh M.J. (2016). Acid-Sensing Ion Channel 1a Contributes to Airway Hyperreactivity in Mice. PLoS ONE, 11(11), e0166089.

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