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Ecosec hlc 8320

Manufactured by Tosoh
Sourced in Japan

The EcoSEC HLC-8320 is a high-performance liquid chromatography (HPLC) system designed for the analysis of polymer samples. It provides accurate and reliable molecular weight and size distribution measurements. The EcoSEC HLC-8320 is a versatile instrument suitable for a wide range of applications in polymer research and quality control.

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5 protocols using ecosec hlc 8320

1

Polymer Characterization by GPC

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Example 61

See FIG. 81

The dissolved fragments were dissolved in CHCl3 at a concentration of 2 mg/mL. The material was then filtered through a 0.2 μm Teflon filter before analysis. GPC analysis was performed on a Tosoh EcoSEC HLC-8320 with dual TSKgel SuperH3000 columns and a chloroform mobile phase. Molecular weight calculations were performed using linear polystyrene standards.

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2

Comprehensive Characterization of Delaminated MXenes

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Scanning electron microscopy (SEM, S-4800, Hitachi, Japan), X-ray diffractometry (XRD, AERIS, Malvern, UK), and Fourier transform infrared spectroscopy (FTIR, Varian 660-IR, VARIAN, USA) were used to characterize the morphology, crystalline properties, and chemical functional groups of the delaminated MXenes. X-ray photoelectron spectroscopy (XPS, Thermo Scientific K-Alpha+, Thermo Fischer Scientific, USA) was used to perform chemical analyses of products obtained after each reaction step to evidence the success of surface modification. Transmission electron microscopy (TEM, JEM-2100F, JEOL, Japan) was used to characterize the morphology of the PE-grafted MXenes. Dynamic light scattering (DLS) and phase analysis light scattering (PALS) were performed to measure the hydrodynamic diameter (dH) and zeta potential of the particles, respectively, using a Zetasizer ZS90 (Malvern, UK). GPC (EcoSEC HLC-8320, Tosoh, Japan) was performed to characterize the Mw of the synthesized PEs. TGA (SDTA851, Mettler Toledo, USA) was used to estimate the amounts of grafted PEs. UV-vis spectroscopy (S-3100, SCINCO, Korea) was used to characterize the particle and dye concentration.
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3

Characterization of Bottlebrush Polymers

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Example 24

For the PS and PLA-based bottlebrush polymers, 15 μL of the crude ROMP reaction mixture were diluted with 15 μL of dioxane. Next, 7 μL of 1M tetrabutylammonium fluoride in THF were added. The solution was incubated for 15 min before being diluted with 1 mL of chloroform, filtered through a 0.2 μm Teflon filter (A ChemTek), and analyzed by chloroform GPC.

GPC analysis was performed in a Tosoh EcoSEC HLC-8320 with dual TSKgel SuperH3000 columns and a chloroform mobile phase.

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4

Copolymer Molecular Weight Characterization

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Copolymer
sample (5 mg/mL) was prepared in tetrahydrofuran and analyzed using
GPC (Tosoh Bioscience EcoSEC HLC-8320: modular system with refractive
index and UV detectors) to find retention time and determine weight
average molecular weight, number average molecular weight, and PDI
of the synthesized copolymer.
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5

PLGA Microspheres Degradation Analysis

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The surface morphology of remaining PLGA microspheres under incubation of 14 and 28 days was characterized using SEM. The microspheres under incubation of 0 and 28 days were dissolved in DMSO and acidized by HCl, and then the content of magnesium in the microspheres was assayed by inductively coupled plasma–atomic emission spectrometer (ICP-AES) (Avio 500, PerkinElmer, Waltham, MA, USA). The pH of supernatants collected from in vitro release assay were tested using electronic pH meter (pH5S, Sanxin, Hainan, China) at 20 °C. The microspheres under incubation of 7, 14, 21, 28 and 35 days were dissolved in tetrahydrofuran (THF) and analyzed by gel permeation chromatography (GPC) (EcoSEC HLC-8320, Tosoh, Tokyo, Japan) (polystyrene as the standard) to show the average molecular weight of degrading PLGA.
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