Log In Sign up
The largest database of trusted experimental protocols

1200s vibratome

Manufactured by Leica
Visit Supplier
Sourced in Germany

The 1200S vibratome is a precision instrument used for sectioning biological samples. It employs a vibrating blade to produce thin, uniform slices of tissue for microscopic analysis and research applications.

Automatically generated - may contain errors

Lab products found in correlation

Flaming brown micropipette puller, by Sutter Instruments (2 mentions) Infrared differential interference contrast video enhanced microscopy, by Olympus (2 mentions) Multiclamp 700b amplifier, by Molecular Devices (2 mentions) Clampfit 10, by Molecular Devices (2 mentions) Alexa fluor 488, by Thermo Fisher Scientific (2 mentions) C fos antibody, by Santa Cruz Biotechnology (1 mentions) Axio zoom v16 microscope, by Zeiss (1 mentions) Lsm 710 confocal, by Zeiss (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Vector Laboratories (1 mentions) H3570, by Thermo Fisher Scientific (1 mentions) A22287, by Thermo Fisher Scientific (1 mentions) Axioscope, by Zeiss (1 mentions) C57bl 6 mice, by Charles River Laboratories (1 mentions) Rc 26, by Warner Instruments (1 mentions) Recording chamber, by Warner Instruments (1 mentions) Isotemp 110 water bath, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Leica Vibratome 1200S Vibratome (VT-1200 Series, Vibratome

20 protocols using 1200s vibratome

1

Slice Preparation and Electrophysiology of BNST

Check if the same lab product or an alternative is used in the 5 most similar protocols
Brains were sectioned at 0.07 (mm/s) on a Leica 1200S vibratome to obtain 300 µm coronal slices of the BNST, which were incubated in a heated holding chamber containing normal, oxygenated aCSF (in mM:124 NaCl, 4.4 KCl, 2 CaCl2, 1.2 MgSO4, 1 NaH2PO4, 10.0 glucose, and 26.0 NaHCO3) maintained at 30 ± 1°C for at least 1 hour before recording. Slices were transferred to a recording chamber (Warner Instruments) submerged in normal, oxygenated aCSF maintained at 28-30°C at a flow rate of 2 ml/min. Neurons of the BNST were visualized using infrared differential interference contrast (DIC) video-enhanced microscopy (Olympus). Borosilicate electrodes were pulled with a Flaming-Brown micropipette puller (Sutter Instruments) and had a pipette resistance between 3-6 MΩ. Signals were acquired via a Multiclamp 700B amplifier, digitized at 10 kHz and analyzed with Clampfit 10.3 software (Molecular Devices, Sunnyvale, CA, USA).
Marcinkiewcz C.A., Mazzone C.M., D’Agostino G., Halladay L.R., Hardaway J.A., DiBerto J.F., Navarro M., Burnham N., Cristiano C., Dorrier C.E., Tipton G.J., Ramakrishnan C., Kozicz T., Deisseroth K., Thiele T.E., McElligott Z.A., Holmes A., Heisler L.K, & Kash T.L. (2016). Serotonin engages an anxiety and fear-promoting circuit in the extended amygdala. Nature, 537(7618), 97-101.
+ Open protocol
+ Expand
2

Brainstem Slicing and Electrophysiology

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Slices were obtained from adult (≥P60) C57/BL6 mice or from Syt7 KOs and wildtype littermates of both sexes (Chakrabarti et al., 2003 (link)). Animals were anesthetized using ketamine/xylazine (100/10 mg/kg) and transcardially perfused with solution composed of in mM: 110 Choline Cl, 2.5 KCl, 1.25 NaH2PO4, 25 NaHCO3, 25 glucose, 0.5 CaCl2, 7 MgCl2, 3.1 Na Pyruvate, 11.6 Na Ascorbate, 0.002 (R,S)-CPP, 0.005 NBQX, oxygenated with 95% O2 / 5% CO2, kept at 35°C. The back of the skull was removed and the hind brain was dissected by making a cut between the border of the cerebellum and midbrain. The dura was carefully removed from the hindbrain and the cut face was glued down. 250 μm thick coronal sections of the brainstem were made on a Leica 1200S vibratome and were then transferred to a holding chamber with ACSF containing in mM: 127 NaCl, 2.5 KCl, 1.25 NaH2PO4, 25 NaHCO3, 25 glucose, 1.5 CaCl2, 1 MgCl2, and allowed to recover at 35°C for at least 20 minutes before cooling to room temperature. For experiments involving an AAV, electrophysiology was performed 10–20 days after injections.
Turecek J, & Regehr W.G. (2019). Neuronal regulation of fast synaptotagmin isoforms controls the relative contributions of synchronous and asynchronous release. Neuron, 101(5), 938-949.e4.
+ Open protocol
+ Expand
3

Acute Hippocampal Slice Preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Postnatal day (P) 15-20 mice were anesthetized with isoflurane and quickly decapitated. The brain was rapidly removed and submerged in ice-cold, low [Ca2+]o, high sucrose dissecting solution continuously oxygenated with carbogen (95%-5% O2-CO2) gas. Horizontal whole brain slices (300 μm) were made with a Leica 1200 S vibratome. Slices containing both hippocampus and entorhinal cortex were recovered in oxygenated normal artificial cerebrospinal fluid (ACSF) for 1 h at 32 C, and then stored at room temperature until use. The standard dissecting solution contained (mM): 87 NaCl, 2.5 KCl, 25 NaHCO3, 1.25 NaH2PO4, 4 MgCl2, 0.5 CaCl2, 10 D-glucose, and 75 sucrose. During recordings, brain slices were continuously perfused with ACSF (3 ml/min flow rate) containing (mM): 125 NaCl, 2.5 KCl, 25 NaHCO3, 1.25 NaH2PO4, 1 MgCl2, 2 CaCl2, 25 D-glucose, and 10 sucrose. Both solutions were maintained at pH 7.4 by continuous oxygenation with carbogen gas. All chemicals were obtained though Fisher Scientific unless otherwise specified.
Myers T.L., Gonzalez O.C., Stein J.B, & Bazhenov M. (2018). Characterizing Concentration-Dependent Neural Dynamics of 4-Aminopyridine-Induced Epileptiform Activity. Epilepsy journal, 4(2), 128.
+ Open protocol
+ Expand
4

Preparation of Cerebellar Nuclei Slices

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mice were anesthetized with ketamine / xylazine / acepromazine and transcardially perfused with warm choline ACSF solution (34°C) containing in mM: 110 Choline Cl, 2.5 KCl, 1.25 NaH2PO4, 25 NaHCO3, 25 glucose, 0.5 CaCl2, 7 MgCl2, 3.1 Na Pyruvate, 11.6 Na Ascorbate, 0.002 (R,S)-CPP, 0.005 NBQX, oxygenated with 95% O2 / 5% CO2. To prepare sagittal cerebellar nuclei slices, the hindbrain was removed, a cut was made down the midline of the cerebellum and brainstem, and the halves of the cerebellum were glued down to the slicing chamber. Sagittal slices (170 μm) were cut using a Leica 1200S vibratome in warm choline ACSF (34°C). Slices were transferred to a holding chamber with warm ACSF solution (34°C) containing in mM: 127 NaCl, 2.5 KCl, 1.25 NaH2PO4, 25 NaHCO3, 25 glucose, 1.5 CaCl2, 1 MgCl2 and were recovered at 34°C for 10 minutes before being moved to room temperature for another 20–30 mins until recordings begin.
Wu S., Wardak A., Khan M.M., Chen C.H, & Regehr W.G. (2023). Implications of variable synaptic weights for rate and temporal coding of cerebellar outputs. bioRxiv.
+ Open protocol
+ Expand
5

Synaptic Inhibition in Medial Central Amygdala

Check if the same lab product or an alternative is used in the 5 most similar protocols
Briefly, rats were anesthetized with isoflurane (3–5%) followed by rapid decapitation and immediate removal of the brain into an ice-cold high sucrose cutting solution of the following composition (in mM): Sucrose 206; KCl 2.5; CaCl2 0.5; MgCl2 7; NaH2PO4 1.2; NaHCO3 26; glucose 5; HEPES 5; pH 7.4. Coronal slices containing the CeA (400 μm) were cut on a Leica 1200S vibratome (Buffalo Grove, IL), incubated in an interface configuration for 5 – 17 min, and then submerged and continuously superfused (flow rate of 2–4 ml/min) with 95% O2/5% CO2 equilibrated room temperature artificial cerebrospinal fluid (aCSF) of the following composition (in mM): NaCl 130; KCl 3.5; NaH2PO4 1.25; MgSO4·7H2O 1.5; CaCl2 2.0; NaHCO, 24; glucose 10; pH 7.4. All recordings were performed 1 – 8 hours after slice preparation in neurons from the medial subdivision of the CeA, and each experimental group contained neurons from at least 3 different animals. Inhibitory neurotransmission was pharmacologically isolated with 20 μM DNQX (to block AMPA receptors), 30 μM DL-AP5 (to block NMDA receptors), and 1 μM CGP 55845A (to block GABAB receptors). All drugs were constituted in aCSF and applied by bath superfusion.
Kirson D., Oleata C.S, & Roberto M. (2019). Taurine suppression of central amygdala GABAergic inhibitory signaling via glycine receptors is disrupted in alcohol dependence. Alcoholism, clinical and experimental research, 44(2), 445-454.
+ Open protocol
+ Expand
6

Cerebellar Slice Preparation for Physiology

Check if the same lab product or an alternative is used in the 5 most similar protocols
WT or Doc2b KO mice of both sexes were used for physiology experiments. Animal age varied across experiments (P12 and P40-P50 for PC to DCN mIPSCs, P7-8 for PC to PC mIPSCs, P18-P20 for PC to DCN minimal stimulation and burst stimulation, and P13-15 and P60-P80 for PC to DCN train experiments). Animals older than P20 were anesthetized with ketamine/xylazine/ acepromazine and transcardially perfused with warm choline-ACSF solution containing in mM: 110 Choline Cl, 2.5 KCl, 1.25 NaH2PO4, 25 NaHCO3, 25 glucose, 0.5 CaCl2, 7 MgCl2, 3.1 Na-Pyruvate, 11.6 Na-Ascorbate, 0.005 NBQX, and 0.0025 (R)-CPP, oxygenated with 95% O2/5% CO2. To prepare sagittal slices of the cerebellum, the hindbrain was first removed, a cut was made down the midline of the cerebellum, and the two halves of the cerebellum were glued down to the slicing chamber. 150–200 µm thick sagittal slices were cut with a Leica 1200S vibratome in warm choline-ACSF. Slices were transferred to a standard ACSF solution containing, in mM: 127 NaCl, 2.5 KCl, 1.25 NaH2PO4, 25 NaHCO3, 25 glucose, 1.5 CaCl2, and 1 MgCl2 maintained at 34–35°C for 10–12 min and then moved to room temperature for 20–30 min before beginning recordings. Procedures involving animals were approved by the Harvard Medical Area Standing Committee on Animals.
Khan M.M, & Regehr W.G. (2020). Loss of Doc2b does not influence transmission at Purkinje cell to deep nuclei synapses under physiological conditions. eLife, 9, e55165.
+ Open protocol
+ Expand
7

Immunohistochemical Quantification of FOS Expression in BNST

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mice were perfused transcardially using 30 ml phosphate buffered saline (PBS) followed by 30 ml 4% paraformaldehyde in PBS. Brains were dissected out and postfixed for 24 hours in 4% paraformaldehyde at 4°C, t hen transferred to 30% sucrose until fully saturated. Slices of the BNST (45 μm) were prepared on a Leica 1200S vibratome and stored in 50/50 glycerol/PBS at −20°C for FOS i mmunohistochemistry. Slices were first washed in PBS, then incubated in 50% methanol and followed by 3% H2O2. Slices were washed in PBS again followed by a 24 hour incubation at 4°C in 0.5% BSA, 0.3% Triton-X100, and c-fos antibody (1:3000; Santa-Cruz). The next day, slices were washed in Tris-HCl, NaCl, Tween (TNT) buffer, incubated in Tris, NaCl blocking (TNB) buffer, and then incubated in goat anti-rabbit HRP-linked IgG (1:200) in TNB buffer for 30 minutes. Slices were washed in TNT, and tyramine signal amplification (TSA) with Cy3 was performed using a kit (Perkin Elmer).
Slices were mounted with Vectashield on glass slices and imaged using a Zeiss AXIO Zoom V16 microscope with ZEN pro 2012 software. FOS-positive cells from at least 3 sections per animal were counted using NIH image software separately in the dorsolateral BNST (dlBNST), dorsomedial BNST (dmBNST) and vBNST.
Marcinkiewcz C.A., Dorrier C.E., Lopez A.J, & Kash T.L. (2014). Ethanol induced adaptations in 5-HT2c receptor signaling in the bed nucleus of stria terminalis: Implications for anxiety during ethanol withdrawal. Neuropharmacology, 89, 157-167.
+ Open protocol
+ Expand
8

Acute Slice Preparation of Mouse Brain

Check if the same lab product or an alternative is used in the 5 most similar protocols
Acute slices were prepared in mice of both sexes (P21-32 unless otherwise indicated). Mice were sacrificed 12–14 days following AAV injections. Animals were anesthetized with ketamine/xylazine (100/10 mg/kg) and transcardially perfused with solution composed of in mM: 110 Choline Cl, 2.5 KCl, 1.25 NaH2PO4, 25 NaHCO3, 25 glucose, 0.5 CaCl2, 7 MgCl2, 3.1 Na Pyruvate, 11.6 Na Ascorbate, 0.002 (R)-CPP, 0.005 NBQX, oxygenated with 95% O2 / 5% CO2, kept at 35°C. For DCN recordings, a cut was made down the midline of the hindbrain, and the cut face of each side was glued to the slicing chamber to generate sagittal slices. For the vestibular nuclei, a cut was made down the midbrain between the cerebellum and cortex and glued to the slicing chamber to generate coronal slices. 250 μm thick sections were made on a Leica 1200S vibratome and were then transferred to a holding chamber with ACSF containing in mM: 127 NaCl, 2.5 KCl, 1.25 NaH2PO4, 25 NaHCO3, 25 glucose, 1.5 CaCl2, 1 MgCl2, and allowed to recover at 35°C for at least 20 minutes before cooling to room temperature.
Turecek J., Jackman S.L, & Regehr W.G. (2017). Synaptotagmin 7 confers frequency invariance onto specialized depressing synapses. Nature, 551(7681), 503-506.
+ Open protocol
+ Expand
9

Acute Hippocampal Slice Preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Postnatal day (P) 15-20 mice were anesthetized with isoflurane and quickly decapitated. The brain was rapidly removed and submerged in ice-cold, low [Ca2+]o, high sucrose dissecting solution continuously oxygenated with carbogen (95%-5% O2-CO2) gas. Horizontal whole brain slices (300 μm) were made with a Leica 1200 S vibratome. Slices containing both hippocampus and entorhinal cortex were recovered in oxygenated normal artificial cerebrospinal fluid (ACSF) for 1 h at 32 C, and then stored at room temperature until use. The standard dissecting solution contained (mM): 87 NaCl, 2.5 KCl, 25 NaHCO3, 1.25 NaH2PO4, 4 MgCl2, 0.5 CaCl2, 10 D-glucose, and 75 sucrose. During recordings, brain slices were continuously perfused with ACSF (3 ml/min flow rate) containing (mM): 125 NaCl, 2.5 KCl, 25 NaHCO3, 1.25 NaH2PO4, 1 MgCl2, 2 CaCl2, 25 D-glucose, and 10 sucrose. Both solutions were maintained at pH 7.4 by continuous oxygenation with carbogen gas. All chemicals were obtained though Fisher Scientific unless otherwise specified.
Myers T.L., Gonzalez O.C., Stein J.B, & Bazhenov M. (2018). Characterizing Concentration-Dependent Neural Dynamics of 4-Aminopyridine-Induced Epileptiform Activity. Epilepsy journal, 4(2), 128.
+ Open protocol
+ Expand
10

Acute Slice Preparation of Mouse Brain

Check if the same lab product or an alternative is used in the 5 most similar protocols
Acute slices were prepared in mice of both sexes (P21-32 unless otherwise indicated). Mice were sacrificed 12–14 days following AAV injections. Animals were anesthetized with ketamine/xylazine (100/10 mg/kg) and transcardially perfused with solution composed of in mM: 110 Choline Cl, 2.5 KCl, 1.25 NaH2PO4, 25 NaHCO3, 25 glucose, 0.5 CaCl2, 7 MgCl2, 3.1 Na Pyruvate, 11.6 Na Ascorbate, 0.002 (R)-CPP, 0.005 NBQX, oxygenated with 95% O2 / 5% CO2, kept at 35°C. For DCN recordings, a cut was made down the midline of the hindbrain, and the cut face of each side was glued to the slicing chamber to generate sagittal slices. For the vestibular nuclei, a cut was made down the midbrain between the cerebellum and cortex and glued to the slicing chamber to generate coronal slices. 250 μm thick sections were made on a Leica 1200S vibratome and were then transferred to a holding chamber with ACSF containing in mM: 127 NaCl, 2.5 KCl, 1.25 NaH2PO4, 25 NaHCO3, 25 glucose, 1.5 CaCl2, 1 MgCl2, and allowed to recover at 35°C for at least 20 minutes before cooling to room temperature.
Turecek J., Jackman S.L, & Regehr W.G. (2017). Synaptotagmin 7 confers frequency invariance onto specialized depressing synapses. Nature, 551(7681), 503-506.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!