Anti tia1

Copper chloride dihydrate (CuCl₂·2H₂O), sodium hydroxide, polyvinylpyrrolidone (PVP-K30), sodium sulfide nonahydrate, and hydrazine monohydrate (N₂H₄·H₂O) were purchased from Aladin (Shanghai, China). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay kit and collagenase IV were purchased from Biofroxx (Einhausen, Germany). The annexin V-fluoresceine isothiocyanate apoptosis detection kit and calcein-AM/PI assay kit were obtained from Yeason (Shanghai, China). SH-mPEG₅₀₀₀ and SH-PEG₂₀₀₀-NHS were purchased from Ponsure Biotechnology (Shanghai, China). Standard ISRIB (M7425) was purchased from AbMole (Houston, TX, USA). Copper ion detection kit was obtained from MesGen Biotechnology (Shanghai, China). Anti-G3BP, anti-TIA-1, anti-CD206, and anti-CD86 primary antibodies were purchased from Proteintech (Wuhan, China). Fluorescent Molecule-conjugated anti-CD206, anti-CD86, anti-CD80, anti-CD3, anti-CD4, and anti-CD8 were obtained from Biolegend (San Diego, CA, USA). Anti-eIF2α and anti-p-eIF2α were purchased from Cell Signaling Technology (Danvers, MA, USA).