Macs miltenyi mouse tumour dissociation kit

Manufactured by Miltenyi Biotec

The MACS Miltenyi Mouse Tumour Dissociation Kit is a laboratory product designed for the dissociation of mouse tumor samples. It provides the necessary reagents and protocols for the effective enzymatic and mechanical breakdown of solid tumor tissue to obtain a single-cell suspension suitable for downstream applications.

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Lab products found in correlation

Magnetic bead, by Miltenyi Biotec (2 mentions) Novocyte d2060r, by Agilent Technologies (1 mentions)

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Tumour dissociation kit (31 citations) Mouse tumour dissociation kit (10 citations) MACS kits (8 citations) Miltenyi Mouse Tumour Dissociation Kit (2 citations)

2 protocols using macs miltenyi mouse tumour dissociation kit

Isolation and Analysis of CAR T Cells from Tumor

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On days 7 and 14 after cell infusion, five to eight mice were killed, and tumours were removed. Tumours were collected, pooled together by the group, homogenised, and then dissociated using the MACS Miltenyi Mouse Tumour Dissociation Kit (Miltenyi Biotec) according to the manufacturer’s instructions. Purified CAR T cells were obtained by sorting (magnetic beads, Miltenyi Biotec) according to the manufacturer’s instructions. For flow cytometry analyses, 500,000 cells for each group were sorted from the isolated tumour-infiltrating CAR T cells. For RNA-seq, two technical replicates of 1 × 10⁶ cells each were sorted from the isolated tumour-infiltrating CAR T cells.

Wang Y., Tong C., Dai H., Wu Z., Han X., Guo Y., Chen D., Wei J., Ti D., Liu Z., Mei Q., Li X., Dong L., Nie J., Zhang Y, & Han W. (2021). Low-dose decitabine priming endows CAR T cells with enhanced and persistent antitumour potential via epigenetic reprogramming. Nature Communications, 12, 409.

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Tumour Characterization and CAR T-cell Analysis

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Mice were sacrificed, and the tumours were collected, homogenised, and dissociated using the MACS Miltenyi Mouse Tumour Dissociation Kit (Miltenyi Biotec) according to the manufacturer’s instructions. Purified CAR T cells were obtained by sorting (magnetic beads, Miltenyi Biotec) based on the manufacturer’s instructions. The phenotype of CAR T cell was detected via flow cytometry (ACEA Novocyte D2060R). Sorted CAR T cells were utilised for metabolism analysis and transmission electron microscopy.

Cao B., Liu M., Wang L., Zhu K., Cai M., Chen X., Feng Y., Yang S., Fu S., Zhi C., Ye X., Zhang J., Zhang Z., Yang X., Zhao M., Wu Q., Xu L., Yang L., Lian H., Zhao Q, & Zhang Z. (2022). Remodelling of tumour microenvironment by microwave ablation potentiates immunotherapy of AXL-specific CAR T cells against non-small cell lung cancer. Nature Communications, 13, 6203.

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