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RAD21 is a laboratory protein that functions as a component of the cohesin complex. The cohesin complex is responsible for holding sister chromatids together during cell division, ensuring the proper segregation of chromosomes. RAD21 plays a crucial role in this process, contributing to the structural integrity and regulation of the cohesin complex.

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Lab products found in correlation

Cenp f, by Novus Biologicals (2 mentions) Nipbl, by Santa Cruz Biotechnology (2 mentions) Rad21, by Abcam (2 mentions) Anti rabbit igg hrp linked antibody, by Cell Signaling Technology (2 mentions) Proliferating cell nuclear antigen (pcna), by Santa Cruz Biotechnology (2 mentions) Alpha tubulin, by Merck Group (2 mentions) Histone h3, by Abcam (2 mentions) Na9340, by GE Healthcare (1 mentions) Na931, by GE Healthcare (1 mentions) Nupage novex 4 12 bis tris protein gel, by Thermo Fisher Scientific (1 mentions) H 210, by Santa Cruz Biotechnology (1 mentions) Nupage mops buffer, by Thermo Fisher Scientific (1 mentions) Smc1a, by Fortis Life Sciences (1 mentions) Stag2, by Santa Cruz Biotechnology (1 mentions) Enhanced chemi luminescence (ecl), by Thermo Fisher Scientific (1 mentions) Immobilon p, by Merck Group (1 mentions)

3 protocols using rad21

1

Immunofluorescence and Western Blot Protocols

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Immunofluorescence was performed using the following primary antibodies: RAD21 (Santa Cruz sc-166973, 1:100), PCNA (Santa Cruz sc-56, 1:100), CENPF (Novus Biologicals NB500–101, 1:100). Secondary antibodies used: Goat anti-Rabbit (Jackson ImmunoResearch 111-165-003, 1:200), Sheep anti-Mouse (Jackson ImmunoResearch 505-605-003, 1:100).
Western blots were performed with the following primary antibodies: RAD21 (Abcam ab992, 1:500 or 1:1000), NIPBL (Santa Cruz sc-374625, 1:400), WAPL (Santa Cruz sc-365189, 1:250), alpha tubulin (Sigma T6074, 1:1,000), Histone H3 (Abcam ab1791, 1:40,000), and CTCF (Santa Cruz sc-271474, 1:500). Secondary antibodies used: Goat anti-Rabbit (Jackson ImmunoResearch 111-165-003, 1.3:7,000 - 1.3:10,000), Goat anti-Mouse (Jackson ImmunoResearch 115-545-003, 1.3:7,000 - 1.3:10,000), Anti-mouse IgG HRP-linked Antibody (Cell Signaling Technologies #7076, 1:5,000), Anti-rabbit IgG HRP-linked Antibody (Cell Signaling Technologies #7074, 1:5,000).
Luppino J.M., Park D.S., Nguyen S.C., Lan Y., Xu Z., Yunker R, & Joyce E.F. (2020). Cohesin promotes stochastic domain intermingling to ensure proper regulation of boundary-proximal genes. Nature genetics, 52(8), 840-848.
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2

Analyzing Cohesin Complex Protein Levels

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Protein was extracted from equal cell numbers in a high urea buffer (48% urea, 15 mM Tris-HCl, pH 7.5, 8.7% glycerin, 1% SDS, 0.004% bromophenol blue, and 143 mM β-mercaptoethanol). Protein levels were measured using the Bradford assay. Equal amounts of protein were loaded on NuPAGE Novex 4–12% Bis-Tris protein gel (NP0321; Life Technologies) and run with NuPAGE MOPS buffer (NP0001; Life Technologies). Proteins were transferred to Immobilon P (IPVH00010; EMD Millipore). Membrane was blocked in 5% nonfat milk in TBST and incubated with antibodies for Rad21 (H-210; Santa Cruz Biotechnology, Inc.), Smc1a (A300-055A; Bethyl Laboratories, Inc.), Smc3 (ab9263; Abcam), Stag2 (J-12; Santa Cruz Biotechnology, Inc.) and Actin B (C4; EMD Millipore). Secondary antibodies used were HRP conjugated (NA9340 and NA931; GE Healthcare). Blot was visualized using ECL (PI34077 and PI34095; Thermo Fisher Scientific).
Mullenders J., Aranda-Orgilles B., Lhoumaud P., Keller M., Pae J., Wang K., Kayembe C., Rocha P.P., Raviram R., Gong Y., Premsrirut P.K., Tsirigos A., Bonneau R., Skok J.A., Cimmino L., Hoehn D, & Aifantis I. (2015). Cohesin loss alters adult hematopoietic stem cell homeostasis, leading to myeloproliferative neoplasms. The Journal of Experimental Medicine, 212(11), 1833-1850.
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3

Immunofluorescence and Western Blot Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunofluorescence was performed using the following primary antibodies: RAD21 (Santa Cruz sc-166973, 1:100), PCNA (Santa Cruz sc-56, 1:100), CENPF (Novus Biologicals NB500–101, 1:100). Secondary antibodies used: Goat anti-Rabbit (Jackson ImmunoResearch 111-165-003, 1:200), Sheep anti-Mouse (Jackson ImmunoResearch 505-605-003, 1:100).
Western blots were performed with the following primary antibodies: RAD21 (Abcam ab992, 1:500 or 1:1000), NIPBL (Santa Cruz sc-374625, 1:400), WAPL (Santa Cruz sc-365189, 1:250), alpha tubulin (Sigma T6074, 1:1,000), Histone H3 (Abcam ab1791, 1:40,000), and CTCF (Santa Cruz sc-271474, 1:500). Secondary antibodies used: Goat anti-Rabbit (Jackson ImmunoResearch 111-165-003, 1.3:7,000 - 1.3:10,000), Goat anti-Mouse (Jackson ImmunoResearch 115-545-003, 1.3:7,000 - 1.3:10,000), Anti-mouse IgG HRP-linked Antibody (Cell Signaling Technologies #7076, 1:5,000), Anti-rabbit IgG HRP-linked Antibody (Cell Signaling Technologies #7074, 1:5,000).
Luppino J.M., Park D.S., Nguyen S.C., Lan Y., Xu Z., Yunker R, & Joyce E.F. (2020). Cohesin promotes stochastic domain intermingling to ensure proper regulation of boundary-proximal genes. Nature genetics, 52(8), 840-848.
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