Acrylamide bis acrylamide solution

Manufactured by Merck Group

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Acrylamide/bis-acrylamide solution is a laboratory reagent used in the preparation of polyacrylamide gels. It is a mixture of acrylamide and N,N'-methylenebisacrylamide, which are used as monomers in the formation of the gel matrix. The solution is commonly used in gel electrophoresis techniques to separate and analyze biomolecules, such as proteins and nucleic acids.

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Acrylamide (504 citations) Bis-acrylamide (111 citations) Acrylamide/bis-acrylamide (26 citations) Acrylamide solution (7 citations) Acrylamide/bis-acrylamide 30% solution (5 citations) 30% acrylamide/bis solution (4 citations) 30% solution of acyrlamide/bis-acrylamide (2 citations)

4 protocols using acrylamide bis acrylamide solution

Glioma Cell Culture and Cytotoxicity Assay

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Minimal essential medium eagle (MEM) with l-glutamine (292 mg/L), fetal bovine serum (FBS), trypsin-EDTA, penicillin, streptomycin were purchased from PAA Laboratories GmbH (Pasching, Austria); calcium-free phosphate buffered saline (PBS) was from Biomed (Lublin, Poland), methylthiazolyl diphenyl-tetrazolium bromide (MTT), dimethyl sulfoxide (DMSO) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Acrylamide/bis-acrylamide solution, ammonium persulfate, bovine serum albumin, brilliant blue R, bromophenol blue, calcium chloride, glycine, glycerol, TMZ, sodium dodecyl sulfate (SDS), N,N,N'N'-tetramethylethylenediamine (TEMED), trichloroacetic acid, triton X-100, trizma base were obtained from Sigma-Aldrich (St. Louis, MO, USA). The scintillation cocktail was purchased from PerkinElmer (Boston, MA) and methyl-³H thymidine from MP Biomedicals, Inc. (Irvine, USA). All other chemicals were of ultrapure grade and obtained from Sigma-Aldrich (St. Louis, MO, USA).

Moskwa J., Borawska M.H., Markiewicz-Zukowska R., Puscion-Jakubik A., Naliwajko S.K., Socha K, & Soroczynska J. (2014). Polish Natural Bee Honeys Are Anti-Proliferative and Anti-Metastatic Agents in Human Glioblastoma multiforme U87MG Cell Line. PLoS ONE, 9(3), e90533.

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Cell Viability Assay Protocol

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Minimal essential medium eagle (MEM) with l-glutamine (292 mg/L), fetal bovine serum (FBS), trypsin-EDTA, penicillin, streptomycin were purchased from PAA Laboratories GmbH (Pasching, Austria); calcium-free phosphate buffered saline (PBS) was from Biomed (Lublin, Poland), methylthiazolyl diphenyl-tetrazolium bromide (MTT), dimethyl sulfoxide (DMSO) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Acrylamide/bis-acrylamide solution, ammonium persulfate, bovine serum albumin, brilliant blue R, bromophenol blue, calcium chloride, glycine, glycerol, sodium dodecyl sulfate (SDS), N,N,N’N’-tetramethylethylenediamine (TEMED), trichloroacetic acid, triton X-100, trizma base were obtained from Sigma-Aldrich (St. Louis, MO, USA). Prestained protein standard from BioRad. The scintillation cocktail was purchased from PerkinElmer (Boston, MA) and methyl-³H thymidine from MP Biomedicals, Inc. (Irvine, USA). Solutions: 7 (200 μg/ml 5,5’,6,6’-tetrachloro-1,1’,3,3’-tetra-ethylbenzimidazolocarbocyanine iodide (JC-1) and solution 8 (1 μg/ml DAPI in PBS) to NucleoCounter assays were obtained from ChemoMetec, Denmark. All other chemicals were of ultrapure grade and obtained from Sigma-Aldrich (St. Louis, MO, USA).

Borawska M.H., Naliwajko S.K., Moskwa J., Markiewicz-Żukowska R., Puścion-Jakubik A, & Soroczyńska J. (2016). Anti-proliferative and anti-migration effects of Polish propolis combined with Hypericum perforatum L. on glioblastoma multiforme cell line U87MG. BMC Complementary and Alternative Medicine, 16, 367.

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Quantification of FOXO Protein Levels

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Midguts and fat bodies (associated with the abdominal cuticle) were
dissected, transferred to 12.5% trichloroacetic acid, homogenized with glass
beads (#G8772) and spun for 15 min at 14,000 rpm at 4°C. Pellets were
washed twice with 1M Tris and the resulting pellet was resuspended in
50μl of LDS sample buffer (50% LDS sample buffer, 100 mM DTT, in
nuclease-free water). Protein samples were then separated in 8% Poly-Acrylamide
gels (Acrylamide/Bis-Acrylamide solution, Sigma A7168) following
manufacturer’s instructions, and transferred to a nitrocellulose
membrane. Membranes were blocked, incubated at 4°C overnight with primary
antibodies (anti-dFOXO 1:5000, anti-Actin 1:1000) in 5% skimmed milk (Millipore
#70166), washed and probed with secondary antibodies (1:10000) for 2h at room
temperature. Details about the antibodies used in this study are provided in the
Supplementary
Data. Anti-dFOXO and anti-Actin were applied sequentially to the same
membrane. Densitometric analysis of blot images was carried out using Fiji
software^{86 (link)}.

Corrales G.M., Li M., Svermova T., Goncalves A., Voicu D., Dobson A.J., Southall T.D, & Alic N. (2022). Transcriptional memory of dFOXO activation in youth curtails later-life mortality through chromatin remodelling and Xbp1. Nature aging, 2, 1176-1190.

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Fabrication and Characterization of Hybrid Biomaterial

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ε-Caprolactone (ε-CL), poly(vinyl alcohol) (PVA MW 14KDa), methyl thiozol tetrazolium (MTT), poly(ethylene glycol) methyl ether (MPEG, MW = 4000), stannous octoate (Sn(Oct)₂, Sigma, St Louis, MO, USA) isophorone diisocyanate (IPDI), Pluronic F 127 triblock copolymer, Tris-HCl, tricine, TEMED, ammonium per sulphate, 40% acrylamide-bis acrylamide solution, sodium dodecyl sulphate and ultra-low molecular weight protein marker were acquired from Sigma Aldrich (St Louis, MO, USA). Sodium azide was obtained from UniLab, Republic of South Africa. P11 hexapeptide (Sequence H-S-D-V-H-K, MW 721 Da) and FITC-p11 hexapeptide (Sequence FITC-Ah_x-H-S-D-V-H-K, MW 1224 Da) were obtained from Peptorn Private LTD (Daejeon, Korea). PBS 1X was purchased from Merck (New York, NY, USA). Retinal pigment epithelial cells (RPE-1) were obtained from ATCC (Manassas, VA, USA). Dulbecco’s modified Minimum Essential Medium (MEM), Trypsin EDTA, FBS and penicillin/streptomycin solutions were purchased from Sigma Aldrich (St Louis, MO, USA). PLGA with monomer ratio (lactic acid and glycolic acid) of 75/25 was obtained as a gift from PURASORB, Holland. Tween^® 80, dichloromethane and petroleum ether were purchased from Merck (Darmstadt, Germany).

du Toit L.C., Choonara Y.E, & Pillay V. (2021). An Injectable Nano-Enabled Thermogel to Attain Controlled Delivery of p11 Peptide for the Potential Treatment of Ocular Angiogenic Disorders of the Posterior Segment. Pharmaceutics, 13(2), 176.

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