L cysteine
Manufactured by Nacalai Tesque
Sourced in Japan
L-cysteine is an amino acid used in various laboratory applications. It serves as a building block for proteins and plays a role in the synthesis of other important biomolecules.
Automatically generated - may contain errors
Lab products found in correlation
Glutathione (gsh), by Fujifilm (2 mentions)
Tapi 2, by Peptide Institute (2 mentions)
Bafilomycin a1, by Cayman Chemical (2 mentions)
L tyrosine, by Nacalai Tesque (1 mentions)
L methionine, by Tokyo Chemical Industry (1 mentions)
Glycine, by Nacalai Tesque (1 mentions)
L aspartic acid sodium salt, by Nacalai Tesque (1 mentions)
L histidine monohydrochloride monohydrate, by Nacalai Tesque (1 mentions)
L lysine monohydrochloride, by Nacalai Tesque (1 mentions)
L isoleucine, by Kanto Chemical (1 mentions)
L leucine, by Kanto Chemical (1 mentions)
L asparagine monohydrate, by Kanto Chemical (1 mentions)
L threonine, by Kanto Chemical (1 mentions)
L valine, by Kanto Chemical (1 mentions)
L phenylalanine, by Nacalai Tesque (1 mentions)
L serine, by Nacalai Tesque (1 mentions)
L glutamic acid monosodium salt, by Merck Group (1 mentions)
Citric acid monohydrate, by Merck Group (1 mentions)
Winlab32, by PerkinElmer (1 mentions)
Optima 8300, by PerkinElmer (1 mentions)
9 protocols using l cysteine
1
Cysteine Enantiomers Preparation and Storage
Check if the same lab product or an alternative is used in the 5 most similar protocols
l -Cysteine (CAS 52-90-4, lots 105XW24 and 105XW29) supplied by Ajinomoto Co., Inc. (Kawasaki, Japan) and d -cysteine (lot 13033/1) supplied by BIOSYNTH AG (Switzerland) were stored at room temperature under light-shielded conditions. Water for injection (lots 2I99, IG94, and 2F74, Otsuka Pharmaceutical Factory Inc., Tokushima, Japan) and methylcellulose (lot M8G3001, Nacalai Tesque, Inc., Tokyo, Japan) were used to prepare the vehicles for l -cysteine and d -cysteine.
+ Expand
2
Amino Acid Assay Buffer Preparation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The assay buffer consisted of 10 mm 4‐(2‐hydroxyethyl)‐1‐piperazineethanesulfonic acid (HEPES), 130 mm NaCl, 10 mm glucose, 5 mm KCl, 2 mm CaCl2, and 1.2 mm MgCl2 (pH adjusted to 7.4 with NaOH). The ligands were diluted with the assay buffer at the desired concentrations.
Amino acids were obtained from commercial sources as follows.l ‐cysteine, l ‐aspartic acid sodium salt, l ‐phenylalanine, glycine, l ‐histidine monohydrochloride monohydrate, l ‐lysine monohydrochloride, l ‐proline (l ‐Pro), l ‐arginine (l ‐Arg) hydrochloride, l ‐serine, and l ‐tyrosine were purchased from Nacalai Tesque (Kyoto, Japan); l ‐alanine (l ‐Ala), l ‐isoleucine, l ‐leucine, l ‐asparagine monohydrate, l ‐glutamine (l ‐Gln), l ‐threonine, l ‐valine, and l ‐tryptophane were purchased from Kanto Chemical (Tokyo, Japan); l ‐glutamic acid monosodium salt was purchased from Sigma–Aldrich Japan (Tokyo, Japan); and l ‐methionine was obtained from Tokyo Chemical Industry (Tokyo, Japan).
Amino acids were obtained from commercial sources as follows.
3
Trace Metal Analysis Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The following reagents were used in this study: nitric acid (65%), citric acid monohydrate (EMSURE® Merck, Germany); palladium matrix modifier and magnesium matrix modifier (10.0 ± 0.3 g/l each) Fluka (Sigma Aldrich), L-cysteine (98% Lot No. M4E8787) Nacalai Tesque (Kyoto, Japan); certified 5 mg/L multi-element ICP standard (Lot Nos. M2-MEB655760), certified 100.01 ± 0.64 μg/mL Hg (Lot No. H2HG02113R), 100.02 ± 0.40 μg/mL Pb (Lot No. J2PB03070), 10.005 ± 0.071 μg/mL As (Lot No. H2AS02109) standards from INORGANIC VENTURES (Christiansburg, USA) traceable to NIST and pH buffers 4 and 7 (VMR chemicals, Belgium). Equipment used were: PerkinElmer® Optima 7300 DV ICP-OES interfaced to WinLab32™ version 5.5 (PerkinElmer®, Inc. Norwalk USA); PerkinElmer® Optima 8300 DV ICP-OES interfaced to Syngistix™ version 3.0 (PerkinElmer®, Shelton CT USA); GF-AAS model AA280Z (Agilent Technologies, Inc. Australia) with Zeeman background correction feature; and CV-AFS model PE-1000 (Nippon Instruments Corporation, Takatsuki Japan); HI9811-5 meter (HANNA Instruments, Romania).
4
Synthesis and Characterization of Eudesmane Lactones
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Alantolactone (catalog number: S8318; Selleck Chemicals, Houston, TX, USA), bafilomycin A1 (item number: 119038; Cayman Chemical, Ann Arbor, MI, USA), costunolide (product number: 032-13731; Wako Pure Chemical Industries, Osaka, Japan), glutathione (product number: 073-02013; FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan), L-cysteine (product number: 10309-41: NACalai Tesque), Z-Leu-Leu-Leu-H (aldehyde) (also known as MG-132) (code number: 3175-v; Peptide Institute, Osaka, Japan), NAC (product number: 00512-84; NACalai Tesque), parthenolide (catalog number: P0667-5MG; Sigma, St. Louis, MO, USA), and TAPI-2 (code number: INH-3852-PI; Peptide Institute) were purchased as commercial products. (11S)-2α-bromo-3-oxoeudesmano-12,6α-lactone (also known as santonin-related compound 2: SRC2) (1 ), (11S)-3-oxoeudesmano-12,6α-lactone (2 ), 2α-bromo-3-oxoeudesm-11(13)-eno-12,6α-lactone (3 ), 3-oxoeudesm-11(13)-eno-12,6α-lactone (4 ), (11S)-3-oxoeudesm-1-eno-12,6α-lactone (5 ), (11S)-3β-hydroxyeudesm-1-eno-12,6α-lactone (6 ), 3-oxoeudesma-1,11(13)-dieno-12,6α-lactone (also known as (+)-tuberiferin) (7 ), and 3β-hydroxyeudesma-1,11(13)-dieno-12,6α-lactone (8 ) were synthesized as previously described [36 (link),69 (link),70 (link)].
5
Antioxidant Assessment of Chemical Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Edaravone (3-methyl-1-phenyl-5-pyrazolone),
1-decyl-3-methylimidazolium chloride, 1-butyl-1-methylpyrrolidinium
chloride, tetrabutylphosphonium chloride (80% in water), 1,1-diphenyl-2-picrylhydrazyl
(DPPH) free radical, phenacetin, and 2,3,5-triphenyltetrazolium chloride
(TTC) were purchased from Tokyo Chemical Industry (Tokyo, Japan).
Choline bicarbonate (∼80% in water) was bought from Sigma-Aldrich
(St. Louis, MO, USA). Chloroform-d was purchased
from Fujifilm Wako Pure Chemical (Osaka, Japan). Sodium hydrogen sulfite
andl -cysteine were bought from Nacalai Tesque (Kyoto, Japan).
All other chemicals were of the highest commercially available grade.
1-decyl-3-methylimidazolium chloride, 1-butyl-1-methylpyrrolidinium
chloride, tetrabutylphosphonium chloride (80% in water), 1,1-diphenyl-2-picrylhydrazyl
(DPPH) free radical, phenacetin, and 2,3,5-triphenyltetrazolium chloride
(TTC) were purchased from Tokyo Chemical Industry (Tokyo, Japan).
Choline bicarbonate (∼80% in water) was bought from Sigma-Aldrich
(St. Louis, MO, USA). Chloroform-d was purchased
from Fujifilm Wako Pure Chemical (Osaka, Japan). Sodium hydrogen sulfite
and
All other chemicals were of the highest commercially available grade.
6
Cucurbitacin B Inhibition Study
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cucurbitacin B (Tokyo Chemical Industries Co., Ltd., Tokyo, Japan), bafilomycin A1 (Cayman Chemical Company, Ann Arbor, MI, USA), MG-132 (Peptide Institute, Osaka, Japan), TAPI-2 (Peptide Institute), N-acetyl-L-cysteine (Nacalai Tesque), glutathione (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan), L-cysteine (Nacalai Tesque), ascorbic acid (Nacalai Tesque), Trolox (FUJIFILM Wako Pure Chemical Corporation), cytochalasin D (Cayman Chemical Company), and jasplakinolide (Nacalai Tesque) were purchased for the present study. Human recombinant TNF-α was provided by Dainippon Pharmaceutical Co., Ltd. (Osaka, Japan).
7
Comprehensive Heavy Metal and Pesticide Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For the heavy metal standard, the 1000 mg/kg (SCP Science, Baie-D'Urfé, QC, Canada) product was used. The standards for lead, arsenic, and cadmium were diluted using 0.5 M nitric acid (Merck, Germany) before use. The standard for mercury was diluted using 0.001% l -cysteine (98%, Nacalai Tesque Inc., Kyoto, Japan). HG-MHT and HG-BHT (NIC., Japan) were used as the additives for the analyses of mercury.
The standards used for testing pesticide residues were α-benzene hexachloride (BHC), β-BHC, γ-BHC, δ-BHC, aldrin, dieldrin, endrin, P.P-dichlorodiphenyldichloroethane (DDD), P.P-dichlorodiphenyldichloroethylene (DDE), O.P-dichlorodiphenyltrichloroethane (DDT), and for P.P-DDT, Dr. Ehrenstorfer (LGC Standards GmbH, Wesel, Germany) products were used. The solvents used in extraction and refining were acetone and methylene chloride, and n-hexane from Merck was used for pesticide residue analysis, while the Florisil Cartridges (1000 mg, 6 mL) for refining were from Waters (USA).
The standards used for testing pesticide residues were α-benzene hexachloride (BHC), β-BHC, γ-BHC, δ-BHC, aldrin, dieldrin, endrin, P.P-dichlorodiphenyldichloroethane (DDD), P.P-dichlorodiphenyldichloroethylene (DDE), O.P-dichlorodiphenyltrichloroethane (DDT), and for P.P-DDT, Dr. Ehrenstorfer (LGC Standards GmbH, Wesel, Germany) products were used. The solvents used in extraction and refining were acetone and methylene chloride, and n-hexane from Merck was used for pesticide residue analysis, while the Florisil Cartridges (1000 mg, 6 mL) for refining were from Waters (USA).
8
EV Protein Extraction and Digestion Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Separated EV fractions were lysed with lysis buffer (12 mM sodium deoxycholate, 12 mM sodium lauroyl sarcosinate, 50 mM ammonium bicarbonate (ABC)), and then the mixed samples were vortexed for 5 min at room temperature followed by spin down and boiling for 10 min at 95°C. The samples were reduced with 10 mM tris(2-carboxyethyl)phosphine (TCEP) (# 209-19861 WAKO) for 30 min at 37°C and alkylated with 20 mM iodoacetamide (# 19302-54 Nacalai Tesque) for 30 min at 37°C in the dark. Subsequently, L-cysteine (# 1030912 Nacalai Tesque) was added to the samples to a final concentration of 21 mM, and then the mixed sample was incubated for 10 min at room temperature. The samples were digested with 2 μL of 1 mAU/μL Lys C (#121-02541 WAKO) and 0.5 μg/μL trypsin solution (#202-20081 WAKO) overnight at 37°C. The digested peptides were desalted via the stop-and-go-extraction tip (StageTip) protocol (Rappsilber et al., 2007 (link)), dried via vacuum centrifugation, and resuspended in 2% acetonitrile 1% trifluoroacetic acid for liquid chromatography and tandem mass spectrometry (LC-MS/MS) processing.
9
Seafood Heavy Metal Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fish samples were obtained from the Binuangeun Fish Auction (latitude: -6.838101°, longitude: 105.883637°) in Lebak, Banten, in September 2019. Fifteen individuals of D. russelli and thirty individuals of M. cordyla were rinsed using clean water to remove dirt and directly placed in a zip-lock plastic bag and freeze until further analysis. In this study, the samples analyzed were meat, gonad, liver, eggs, digestive content, and digestive tract in the fish sample. The samples were taken to Biogeochemistry Laboratory at Research Center for Oceanography, Indonesian Institute of Sciences (RCO-LIPI). All reagents used were of analytical-reagent grade. The solutions prepared using ultra-pure water (Milli-Q). Mercury Standard solution 1.000 mg/L (Merck) and L-Cysteine from Nacalai Tesque Inc. Japan.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!