Ftir spectrophotometer

Manufactured by Bruker

Sourced in Germany, United States, United Kingdom, Japan

The FTIR spectrophotometer is an analytical instrument used to identify and quantify the chemical composition of samples. It measures the absorption or transmission of infrared light through a sample, providing a unique spectrum that can be used to detect and analyze various organic and inorganic compounds.

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Lab products found in correlation

Tensor 27, by Bruker (3 mentions) 400 mhz spectrometer, by Bruker (3 mentions) Mercury vx 300 nmr spectrometer, by Agilent Technologies (2 mentions) Silica gel 60 f254, by Merck Group (2 mentions) Avance 400, by Bruker (2 mentions) Hptlc scanner 3, by CAMAG (2 mentions) Gas chromatograph mass spectrometer qp 1000 ex, by Shimadzu (2 mentions) 4 nitrobenzenesulfonylchloride, by Merck Group (2 mentions) Zetasizer nano zs90, by Malvern Panalytical (2 mentions) Single reflection diamond attenuated total reflectance cell, by Specac (2 mentions) Dimethyl sulfoxide (dmso), by Merck Group (2 mentions) Hydrochloric acid (hcl), by Merck Group (2 mentions) Supra 55vp, by Zeiss (2 mentions) Dsa100, by Krüss (1 mentions) Jsm 5300, by JEOL (1 mentions) Chn 2400, by PerkinElmer (1 mentions) Formaldehyde, by BD (1 mentions) Mounting media, by BD (1 mentions) Mouse anti cox 2, by Santa Cruz Biotechnology (1 mentions) 1 chloro 2 4 dinitrobenzene, by Merck Group (1 mentions)

88 protocols using ftir spectrophotometer

Characterization of Electrospun Nanofibers

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Scanning electron microscopy (SEM) was used for evaluation of the morphology of the nanofibers to determine the optimum condition of electrospinning process. In brief, the nanofibrous scaffolds were initially dried and the samples were then mounted on aluminum stubs, followed by sputter-coating with gold, and imaging using a JEOL SEM device (JSM5300, JEOL Ltd., Tokyo, Japan).
FT-IR spectroscopy was performed to study the chemical bonds of the scaffolds and define probable interactions between components in the blends. In brief, specimens were combined with potassium bromide powder and pressed to create a round tablet. The FT-IR spectra of the specimens were then acquired by a FT-IR spectrophotometer (Bruker, Germany) and analyzed in the range of 400–4000 cm⁻¹.
The hydrophilicity of the fabricated webs was assessed by measuring the contact angle of distilled water drop on the web, using Kruss drop shape analyzer (DSA 100). The webs were cut into 100 mm² and fixed into the custom made sample holder. The shape of a single drop of distilled water (volume∼2 μL) on the web surfaces was recorded using the camera attached to the drop shape analyzer after 3 s. The contact angle was measured by the sessile drop approximation of the inbuilt software of the instrument.

Shadman-Manesh V., Gholipour-Kanani A., Najmoddin N, & Rabbani S. (2023). Preclinical evaluation of the polycaprolactone-polyethylene glycol electrospun nanofibers containing egg-yolk oil for acceleration of full thickness burns healing. Scientific Reports, 13, 919.

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FTIR Analysis of Drug-Excipient Compatibility

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FTIR spectra of pure drugs and formulated FDT containing drugs were recorded on FTIR Spectrophotometer (Bruker, USA). This study generally includes FTIR spectroscopy and these are generally performed to confirm the drug-excipient compatibility. FTIR spectra of samples were recorded in scanning range of 4000 to 600 cm⁻¹ and the resolution was 1 cm⁻¹. FTIR scans were then evaluated for shifting and masking and appearance of new peaks due to drug-excipient incompatibility [19 (link)].

Sharma D., Singh G., Kumar D, & Singh M. (2015). Formulation Development and Evaluation of Fast Disintegrating Tablets of Salbutamol Sulphate, Cetirizine Hydrochloride in Combined Pharmaceutical Dosage Form: A New Era in Novel Drug Delivery for Pediatrics and Geriatrics. Journal of Drug Delivery, 2015, 640529.

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Formulation Compatibility Analysis via FTIR

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The drug interaction and the composition compatibility within the formulation ingredients were determined using FT-IR analysis. The IR spectrum was performed by using a (Bruker FTIR) spectrophotometer. The samples were recorded using the conventional KBr pellet method. The samples were scanned in the region between 4000 and 400cm-1 with a resolution of about 4cm -1 .

Sundar V.D., Divya P., & Dhanaraju M.D. (2020). Design Development and Characterisation of Tramadol Hydrochloride Loaded Transethosomal Gel Formulation for Effective Pain Management. Indian Journal of Pharmaceutical Education and Research, 54(2s), s88-s97.

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Isolation and Characterization of Embelin from Embelia ribes

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Embelia ribes was collected from the Koyna region, Maharashtra, and embelin, a major phytoconstituent, was isolated from the fruits. Briefly, the fruits of E. ribes were dried, coarsely pulverized, and extracted in chloroform using Soxhlet apparatus. By eluting the extract with benzene, embelin was isolated using silica column chromatography (30 ). The isolated embelin was characterized by a chemical test, FT-IR, and ¹H NMR spectral analyses.
A chemical test was performed for confirmation of embelin. Embelin was dissolved in pet ether, and diluted ammonia was added to the solution. The presence of embelin was indicated by a bluish-violet precipitate (31 ).
The FT-IR spectrum was obtained using an FT-IR spectrophotometer (Bruker, Germany). The IR spectrum was recorded by scanning over a wavelength of 400–4,000 cm⁻¹ using the Origin software. The characteristic IR peaks were observed and compared with the reference spectrum of embelin (31 ). ¹H NMR was done by a sophisticated NMR spectrophotometer (Bruker), and the ¹H frequency was 500 MHz.

Jagtap R.R., Garud A., Puranik S.S., Rudrapal M., Ansari M.A., Alomary M.N., Alshamrani M., Salawi A., Almoshari Y., Khan J, & Warude B. (2022). Biofabrication of Silver Nanoparticles (AgNPs) Using Embelin for Effective Therapeutic Management of Lung Cancer. Frontiers in Nutrition, 9, 960674.

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Bacteriocin-Induced Cellular Response Monitoring

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FTIR spectroscopy is capable of monitoring conformational, compositional and quantitative differences of biochemical compounds in microbial cells. Therefore, this technique was used to evaluate the cellular response of bacteriocin-treated and untreated cells as reported by Zoumpopoulou et al. [25 (link)]. Briefly ~ 10⁶ CFU/mL cells of Staph. aureus subsp. aureus ATCC25923 were treated with enterocin LD3 (25 μg/mL) and plantaricin LD4 (90 μg/mL) individually and in combination (115 μg/mL). Similarly, Salm. enterica subsp. enterica serovar Typhimurium ATCC13311 was treated with enterocin LD3 (30 μg/mL) and plantaricin LD4 (100 μg/mL) individually and in combination (130 μg/mL) and incubated at 37 °C, 200 rpm for 10 h. The untreated cells were used as controls. After incubation for 10 h, cell suspensions were washed twice with sterile saline (0.85% NaCl). Infrared absorbance spectra of bacteriocin-treated and untreated target cells were monitored using an FTIR spectrophotometer (Bruker, Bremen, Germany) on diamond-attenuated total reflectance accessory. For spectra acquisition, Opus software was used. The cells were placed in direct contact with the internal reflecting diamond crystal. Multiple scans were obtained to reduce error. Each spectrum was baseline corrected, and the spectral range was set from 500/cm to 4000/cm at a resolution of 8/cm.

Sheoran P, & Tiwari S.K. (2020). Synergistically-acting Enterocin LD3 and Plantaricin LD4 Against Gram-Positive and Gram-Negative Pathogenic Bacteria. Probiotics and Antimicrobial Proteins, 13(2), 542-554.

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In situ FTIR analysis of artifacts

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In situ analyses on the Morigi collection were performed in ER-FTIR mode, using a portable Alpha Bruker FTIR spectrophotometer equipped with an external reflection module for contactless measurements and a DTGS detector. The analysis parameters used are 200 scans, 4 cm⁻¹ resolution, and a range of 7500–375 cm⁻¹. Periodically, a background spectrum was acquired using a flat gold mirror. The measurement area was approximately 6 mm in diameter and the instrument was placed in a frontal position relative to the analysis point, at a working distance of approximately 1–1.5 cm. Fine-tuning of the optimal distance was then achieved by searching for the maximum signal directly in the interferogram using the software. The acquired spectra were processed using pseudo-absorbance [log (1/R); R = reflectance] as the intensity unit.

Geminiani L., Campione F.P., Canevali C., Corti C., Giussani B., Gorla G., Luraschi M., Recchia S, & Rampazzi L. (2023). Historical Silk: A Novel Method to Evaluate Degumming with Non-Invasive Infrared Spectroscopy and Spectral Deconvolution. Materials, 16(5), 1819.

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FT-IR Analysis of Nanoparticle Components

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For further assessment of a possible interactions between the nanoparticles’ components, FT-IR analysis was conducted over the region of 4000–400 cm⁻¹ at room temperature on pure α-arbutin, chitosan, TPP, HA and COLL powders, selected α-arbutin-loaded CSNPs dispersion and its corresponding unloaded CSNPs dispersion applying the KBr disk method, using Bruker FT-IR spectrophotometer (Model 22, Bruker, UK) (Ayumi et al., 2019 (link)).

Hatem S., Elkheshen S.A., Kamel A.O., Nasr M., Moftah N.H., Ragai M.H., Elezaby R.S, & El Hoffy N.M. (2022). Functionalized chitosan nanoparticles for cutaneous delivery of a skin whitening agent: an approach to clinically augment the therapeutic efficacy for melasma treatment. Drug Delivery, 29(1), 1212-1231.

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Characterization of Curcumin Chelates

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The elemental analyses C, and H were performed by using the microanalysis using a PerkinElmer CHN 2400 (USA). The conductivity measurements for curcumin chelates carried out for solution of 1 × 10⁻³ mol/cm³ using dimethylsulfoxide (DMSO) and were measured using Jenway conductivity meter. The electronic absorption spectra of cur complexity were recorded in DMSO solvent within 800–200 nm range using UV/Vis Spectrophotometer. The infrared spectra were recorded on a Bruker FT-IR Spectrophotometer (4000–400 cm⁻¹). The ¹H NMR spectra were recorded on Varian Mercury VX300 NMR spectrometer. The morphological surface were estimated by a scanning (SEM) and transmission (TEM) electron microscopes generated at 20 kV, where the shapes and sizes were visualized using JEOL 100s microscopy. The X-ray diffraction patterns for Cu (II) Cur complexity were recorded on analytical X-ray powder diffraction with secondary monochromatic.

, & Al-Thubaiti E.H. (2023). Antibacterial and antioxidant activities of curcumin/Zn metal complex with its chemical characterization and spectroscopic studies. Heliyon, 9(6), e17468.

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Molecular Structure Analysis of KGM and WPI

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Fourier transform infrared spectroscopy (FT-IR) technology was used to evaluate the molecular structure of KGM and WPI in water phase before and after homogenization. The IR spectra of the samples were recorded by FT-IR spectrophotometer (Bruker Corp, Billerica, Massachusetts, USA) using an attenuated total reflection (ATR) technique. The spectrum was scanned from 4000 cm -1 -900 cm -1 with a resolution of 4 cm -1 . An average of 300 scans was recorded for each sample.

Lu W., Zheng B, & Miao S. (2018). Improved emulsion stability and modified nutrient release by structuring O/W emulsions using konjac glucomannan. Food Hydrocolloids., 81.

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Anti-inflammatory Compound Characterization

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All the starting materials were purchased from Daejung (South Korea) and Alfa-Aesar (Germany). Digital Gallenkamp (Sanyo) apparatus was used to record the melting points of final compounds and was uncorrected. Proton NMR (¹H-NMR) and carbon-13 (¹³C-NMR) spectra were checked using Bruker AM-300 in DMSO-d6 at 300 MHz and 75 MHz, respectively, while using TMS as an internal standard. Alpha Bruker FTIR spectrophotometer (ATR eco ZnSe, νmax in cm⁻¹) was used to record FTIR spectra. All reactions were monitored by thin-layer chromatography (TLC). 3,3-diaminobenzidine peroxidase, an ABC Elite kit, mouse anti-COX-2, mouse anti-p-NF-κB, and mouse anti-Iba-1 were procured from (Santa Cruz Biotechnology, Dallas, TX, USA). H₂O₂, Proteinase K, formaldehyde, mounting media, and PBS tablets were acquired from BDH while secondary antibody goat anti-mouse was purchased from Abcam (Cambridge, UK). Other chemicals were procured from Sigma-Aldrich (St. Louis, MO, USA) like solvents and reagents such as 5,5′-dithiobis (2-nitrobenzoic acid), 1-chloro-2,4-dinitrobenzene, glutathione (GSH), N-(1-naphthyl) ethylenediamine dihydrochloride, and trichloroacetic acid. All chemicals used were of high analytical grade (99% HPLC).

Imran M., Al Kury L.T., Nadeem H., Shah F.A., Abbas M., Naz S., Khan A.U, & Li S. (2020). Benzimidazole Containing Acetamide Derivatives Attenuate Neuroinflammation and Oxidative Stress in Ethanol-Induced Neurodegeneration. Biomolecules, 10(1), 108.

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