Overexpression screening was carried out using various E. coli host strains with appropriate antibiotics in prescribed concentrations, and the ones that yielded optimal amount of target proteins are listed in Supplementary Table 1 . Brief expression and purification protocols for individual proteins are given below. Compositions of the buffer used are in the Supplementary Table 3 . All the preplaced chromatographic columns used in this study are from GE biosciences. Ultracentrifugation devices (Millipore) with various molecular weight cut off were used for protein concentrations.
Ultracentrifugation devices
Manufactured by Merck Group
Ultracentrifugation devices are specialized laboratory equipment used for the separation and analysis of complex biological samples. These devices utilize high-speed centrifugation to separate molecules, cells, or organelles based on their size, density, and sedimentation rate. The core function of ultracentrifugation devices is to provide a precise and controlled environment for the separation and purification of various biological components, enabling researchers to study their properties and interactions.
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2 protocols using ultracentrifugation devices
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Overexpression Screening and Purification Protocols
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Overexpression Screening and Purification Protocols
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Overexpression screening was carried out using various E. coli host strains with appropriate antibiotics in prescribed concentrations, and the ones that yielded optimal amount of target proteins are listed in Supplementary Table 1 . Brief expression and purification protocols for individual proteins are given below. Compositions of the buffer used are in the Supplementary Table 3 . All the preplaced chromatographic columns used in this study are from GE biosciences. Ultracentrifugation devices (Millipore) with various molecular weight cut off were used for protein concentrations.
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