Hydroxy propyl β cyclodextrin hp β cd

The antiviral activity of ICZ against HRVs was detected by in vitro screening of the Screen-Well FDA-Approved Drug Library V2 Version 1.0 (BML-2843-0100; Enzo Life Sciences, Lausanne, Switzerland). ICZ and hydroxypropyl-β-cyclodextrin (HP-β-CD) were purchased from Tokyo Chemical Industry Co. (Tokyo, Japan). Cholesterol, Rupintrivir, and Pleconaril was obtained from Sigma-Aldrich (St. Louis, MO, USA). ICZ was dissolved in dimethyl sulfoxide at a concentration of 1 mM and diluted with cell culture medium for in vitro experiments. HP-β-CD was dissolved in distilled water at a concentration of 100 mg/ml (10%, w/v). The Cholesterol/CD complex solution was prepared by dissolving Cholesterol (2.5–20 mg/ml) in methanol by heating and diluting 100 times with HP-β-CD (10%, w/v) solution in distilled water. CD and Cholesterol/CD solutions were diluted with cell culture medium to appropriate concentrations for in vitro experiments.

Quercetin hydrate (C₁₅H₁₀O₇·xH₂O, Mw 302.24, >96% purity) and hydroxypropyl-β-cyclodextrin (HP-β-CD, Mw ~1380–1500, >99.0% purity) were purchased from Tokyo Chemical Industry (Portland, OR, USA). Polyvinyl alcohol (PVA; Mw 89,000–98,000, 99+% hydrolyzed) was purchased from Sigma Aldrich (Saint Louis, MO, USA). Methanol (MeOH; HPLC grade) and dimethyl sulfoxide (DMSO; AR grade) were purchased from RCI Labscan (Bangkok, Thailand).