Dab detection ihc kit

Manufactured by Abcam

Sourced in United Kingdom, United States, Canada

The DAB Detection IHC Kit is a laboratory reagent used for the detection and visualization of specific target proteins in tissue sections or cell samples through immunohistochemistry (IHC) techniques. The kit contains the necessary components for the chromogenic detection of target antigens, facilitating their localization and analysis within the sample.

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Lab products found in correlation

Tdt apoptosis detection kit, by Takara Bio (3 mentions) Adi aap 470, by Enzo Life Sciences (2 mentions) Anti ps6 antibody, by Cell Signaling Technology (1 mentions) Ab101500, by Abcam (1 mentions) Axioskop 40, by Zeiss (1 mentions) Axiocam mr, by Zeiss (1 mentions) Safranin o fast green, by Merck Group (1 mentions) Axiovision le64, by Zeiss (1 mentions) Anti chop antibody, by Cell Signaling Technology (1 mentions) Anti seh antibody, by Santa Cruz Biotechnology (1 mentions) Anti p21 antibody, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

Rabbit-specific HRP/DAB (ABC) Detection IHC Kit (28 citations) HRP/DAB Detection IHC Kit (27 citations) HRP/DAB (ABC) Detection IHC kit (24 citations) Expose Mouse and Rabbit Specific HRP/DAB Detection IHC Kit (19 citations) Mouse and Rabbit Specific HRP/DAB (ABC) Detection IHC kit (18 citations) DAB kit (16 citations) IHC Detection Kit (13 citations) EXPOSE Rabbit specific HRP/DAB detection IHC kit (12 citations) Mouse and Rabbit Specific HRP/DAB Detection IHC kit (10 citations) IHC kit (8 citations)

8 protocols using dab detection ihc kit

Evaluating TRAIL and Apoptosis in Tumor Samples

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The removed tumors were analyzed immunohistopathologically for TRAIL to demonstrate the presence of the delivered TRAIL and its involvement in anticancer activity. We used a TRAIL antibody (1:100 ADI-AAP-470, Enzo Life Sciences INC, Farmingdale, NY, USA) and a DAB Detection IHC Kit (Abcam, Cambridge, UK). The TRAIL assay was performed on tumor tissues that were removed after two weeks of treatment with hTERT-ADSC.sTRAIL or the combination of hTERT-ADSC.sTRAIL and CPT-11. The stained sections were visualized using a microscope (Olympus, Tokyo, Japan). Low-power (X100) or high-power (X400) fields were examined.
To detect apoptosis, a terminal deoxynucleotidyl transferase–mediated dUTP nick end-labeling (TUNEL) assay was performed. We used a TdT apoptosis detection kit (Takara Bio Inc, Mountain View, CA, USA) and a DAB Detection IHC Kit (Abcam). The TUNEL assay was performed on tumor tissues that were removed after two weeks of treatment with hTERT-ADSC.sTRAIL or the combination hTERT-ADSC.sTRAIL and CPT-11. The stained sections were visualized using a microscope (Olympus, Tokyo, Japan). Low-power (X100) or high-power (X400) fields were examined.

Kim J.H., Oh E., Han Y.S., Lee S.H, & Song Y.S. (2020). Enhanced inhibition of tumor growth using TRAIL-overexpressing adipose-derived stem cells in combination with the chemotherapeutic agent CPT-11 in castration-resistant prostate cancer. Prostate International, 9(1), 31-41.

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Evaluating TRAIL-Induced Apoptosis in Tumor Tissues

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We did the immunohistopathological examination of removed tumors for TRAIL to detect if delivered TRAIL was present and involved in anticancer activity. We used a TRAIL antibody (1:100 ADI-AAP-470; Enzo Life Sciences, Farmingdale, NY) and a DAB Detection IHC Kit (abcam). We did the TRAIL assay for tumor tissues that were removed after 2 weeks treatment of hTERT-ADSC.sTRAIL and hTERT-ADSC.sTRAIL in combination with CPT-11. We visualized the stained sections using a microscope (Olympus, Tokyo, Japan), using low-power (X100) or high-power (X400) fields of slides.
For the detection of apoptosis, we did a terminal deoxynucleotidyl transferase mediated dUTP nick-end labeling (TUNEL) assay. We used a TdT apoptosis detection kit (Takara BIO, Mountain View, CA) and a DAB Detection IHC Kit (abcam). We did the TUNEL assay for tumor tissues, which were removed after 2 weeks of treatment with hTERT-ADSC.sTRAIL and hTERT-ADSC.sTRAIL in combination with CPT-11. We visualized the stained sections using a microscope (Olympus), using low-power (X100) or high-power (X400) fields of slides.

Kim J.H., Oh E., Yun C.W., Lee S.H, & Song Y.S. (2022). Carboxyl Esterase-TRAIL Expressing Human Adipose Stem Cells Inhibit Tumor Growth in Castration-Resistant Prostate Cancer-Bearing Mice with Less Toxicity. Technology in Cancer Research & Treatment, 21, 15330338221093146.

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Immunohistochemical Analysis of pS6 in Ear Tissue

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Ear tissues were fixed in a 10% neutral-buffered formalin, dehydrated, and embedded in paraffin. Paraffin sections (5 μm thick) were blocked (10% normal goat serum in PBS) and incubated with anti-pS6 antibodies (Cell Signaling, Danvers, MA, USA) overnight at 4 °C. Bound primary antibodies were detected with HRP-Conjugate secondary antibodies and a DAB IHC detection kit (Abcam, Cambridge, UK).

Gupta A., Lee K, & Oh K. (2023). mTORC1 Deficiency Prevents the Development of MC903-Induced Atopic Dermatitis through the Downregulation of Type 2 Inflammation. International Journal of Molecular Sciences, 24(6), 5968.

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Quantifying Tumor-Infiltrating Lymphocytes

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Primary tumor paraffin sections of 4 μm were processed for immunochemistry to evaluate CD8⁺ and FOXP3⁺ lymphocytes according to the following protocol: roast, deparaffination, and rehydration before performing heat-mediated antigen retrieval with EDTA buffer (pH 9.0), inactivation of endogenous peroxidase activity with 3% H₂O₂, incubation with antibody against CD8 (ab101500, 1:500; Abcam, Cambridge, UK) or against FOXP3 (ab200334, 1:500; Abcam) at 4°C overnight, exposure to a DAB IHC Detection Kit after incubation with biotinylated secondary antibodies, and counterstaining with Mayer’s hematoxylin solution. An open-source platform for biological-image analysis (Fiji/ImageJ) was used to estimate the densities of CD8⁺ and FOXP3⁺ lymphocytes.

Liu C., Xiao H., Cui L., Fang L., Han S., Ruan Y., Zhao W, & Zhang Y. (2022). Epigenetic-related gene mutations serve as potential biomarkers for immune checkpoint inhibitors in microsatellite-stable colorectal cancer. Frontiers in Immunology, 13, 1039631.

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IVD Tissue Characterization Protocol

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For intact IVDs, post-MRI analysis, a 2 mm wide sagittal tissue segment from the center of the IVD was fixed in periodate lysine paraformaldehyde (PLP) fixative overnight at 4°C. Samples were then washed in PBS and decalcified using Shandon TBD1 Decalcifier solution (ThermoFischer Scientific) over 72 hr at 4°C, changing solution each day. Tissue segments were washed in PBS and placed in 70% ethanol prior to paraffin embedding. Sections of 5 µm were cut and mounted on glass slides. All sections were heated on a hot plate at 55°C for 45 min and deparaffinized and rehydrated. Next, sections were stained with safranin-O/fast green (Sigma-Aldrich, Oakville, ON, Canada) and with antibodies against p16^Ink4a and Ki-67 and counter stained using the DAB detection IHC Kit (ab64264, Abcam, Cambridge, MA) following the manufacturer’s instructions. All images were acquired using a Zeiss Axioskop 40 and an AxioCam MR (Zeiss) and processed using AxioVision LE64 software (Zeiss).

Cherif H., Bisson D.G., Mannarino M., Rabau O., Ouellet J.A, & Haglund L. (2020). Senotherapeutic drugs for human intervertebral disc degeneration and low back pain. eLife, 9, e54693.

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Immunohistochemical Analysis of Cell Signaling Proteins

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The tissue fixation, paraffin embedding, section, and dewaxing were performed as previously described [49 (link)]. Antigen retrieval was performed by heating the sections in 0.01 M citrate buffer (pH 6.0) to 95 °C for 10 min. The immunohistochemistry staining was conducted using horseradish peroxidase (HRP)/3,3′-diaminobenzidine (DAB) Detection IHC kit (Abcam, Cambridge, UK) according to the manufacturer’s instructions. The anti-p21 antibody (Cell Signaling, Danvers, MA, USA, catalog # 64016), anti-Chop antibody (Cell Signaling, catalog # 2895), or anti-sEH antibody (Santa Cruz Biotechnology, Dallas, TX, USA, catalog # sc-166961) was used to probe the target protein in the tissue section. Sections were then counterstained with hematoxylin for 1 min. The staining intensity of p21, Chop, and sEH was analyzed by Image J software using IHC Toolbox.

Wang W., Wagner K.M., Wang Y., Singh N., Yang J., He Q., Morisseau C, & Hammock B.D. (2023). Soluble Epoxide Hydrolase Contributes to Cell Senescence and ER Stress in Aging Mice Colon. International Journal of Molecular Sciences, 24(5), 4570.

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Molecular Characterization of Penile Tissue

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We performed immunostaining of the solute carrier family 2 member 9 (SLC2A9), leucine rick repeat containing 20 (LRRC20), polo like kinase 1 (PLK1), and apoptosis-associated tyrosine kinase (AATK) protein on the cavernosum sinusoids. We performed Masson trichrome (Sigma Diagnostic, St. Louis, MO, USA) staining for collagen on 5-μm tissue sections derived from penile shaft. For the detection of apoptosis, we carried out terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) assay with a terminal deoxynucleotidyl transferase (TdT) apoptosis detection kit (Takara BIO Inc., Mountain View, CA, USA) and a DAB Detection IHC Kit (Abcam, Cambridge, UK). We subjected the sections to incubation with 20-μg/mL proteinase K, 2% hydrogen peroxide to quench endogenous peroxidase activity, digoxigenin-conjugated nucleotides and TdT, and antidigoxigenin peroxidase. We used 0.5% diaminobenzidine/0.01% hydrogen peroxide for staining of the sections and hematoxylin for counterstaining. For the negative control, we used the buffer instead of the TdT enzyme. Quantitative analysis of the stained area calculations was performed using Image J version 1.8.0_172 (nih.gov).

Kim J.H., Yang H.J., Park S., Lee H.J, & Song Y.S. (2023). Differential Gene Expression in the Penile Cavernosum of Streptozotocin-Induced Diabetic Rats. International Neurourology Journal, 27(4), 234-242.

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Immunohistochemical Analysis of Cancer-IgG in Glioma

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The tissue microarray (TMA) used in this study was purchased from Shanghai Outdo Biotech Co., Ltd. (Shanghai, China). All patients were diagnosed with glioma by pathology. The mAb RP215 (sc-69849; Santa Cruz Biotechnology, Santa Cruz, CA, USA) was used to specifically recognize cancer-IgG. Human tissues were stained using mouse and rabbit specific horseradish peroxidase (HRP)/3,3′-diaminobenzidine (DAB) Detection IHC Kit (ab64264; Abcam, Cambridge, UK) according to the manufacturer’s instructions. The immunohistochemical staining score was based on previously published articles. The staining intensity was scored as follows: 0: no staining; 1: weak staining; 2: moderate staining: and 3: strong staining. The positive staining cell rate was scored as follows: 0: 0%–5%; 1: 5%–25%; 2: 26%–50%; 3: 51%–75%; and 4: >75%. A score below three points was considered negative and more than three points as positive.

Wang G., Li H., Pan J., Yan T., Zhou H., Han X., Su L., Hou L, & Xue X. (2021). Upregulated Expression of Cancer-Derived Immunoglobulin G Is Associated With Progression in Glioma. Frontiers in Oncology, 11, 758856.

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