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Nexus x2

Manufactured by Eppendorf
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Sourced in Germany

The Nexus X2 is a high-performance centrifuge designed for a wide range of laboratory applications. It features a compact design, low noise operation, and advanced temperature control capabilities.

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Lab products found in correlation

Gotaq green master mix, by Promega (2 mentions) Phire plant direct pcr master mix, by Thermo Fisher Scientific (1 mentions)

3 protocols using nexus x2

1

Polymorphic Effector Gene Regions in P. cactorum

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Three effector gene regions that are potentially polymorphic were chosen: RXLR6, RXLR7 [19 (link)] and SCR113 [42 (link)]. Only a subset of all P. cactorum isolates were used in this part of the whole study (Table 1). Primer sequences were used according to Chen et al. [19 (link),42 (link)]; their sequences are given in Table 2. The PCR was performed using Phire Plant Direct PCR Mastermix (Thermo Fisher ScientificTM). The concentration of primers in RXLR6 and RXLR7 was 0.2 mM, and in SCR113 it was 1.32 mM. The reaction conditions in the thermocycler (Eppendorf Nexus X2, Eppendorf, Hamburg, Germany) were identical for all three DNA regions except for the annealing phase. The cycling conditions were as follows: 98 °C for 5 min; 35 cycles of 98 °C for 30 s, annealing −54 °C for 15 s for both RXLRs and 55 °C for 30 s for SCR113, 72 °C for 60 s, then 72 °C for 5 min. The PCR product was sequenced by MacroGen Inc. (Seoul, South Korea).
Pánek M., Střížková I., Zouhar M., Kudláček T, & Tomšovský M. (2021). Mixed-Mating Model of Reproduction Revealed in European Phytophthora cactorum by ddRADseq and Effector Gene Sequence Data. Microorganisms, 9(2), 345.
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2

Conditional Knockout Mouse Model for PTHrP Signaling

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PTHrP-creER bacterial artificial chromosome (BAC) mice
have been established and described previously (Mizuhashi et al., 2018 (link)). PPR-floxed (Kobayashi et al., 2002 (link)) and
Rosa26-CAG-loxP-stop-loxP-tdTomato (Ail4: R26R-tdTomato,
JAX007914) mice were obtained from the Jackson laboratory. All procedures were
performed under the Guidelines for the Care and Use of Laboratory Animals
approved by the University of Michigan’s Institutional Animal Care and
Use Committee (IACUC), protocol 7,000 (Ono). The mouse PFE model was created by
crossing PTHrP-creER and PPR-floxed mice,
generating three corresponding groups including
PPRfl/fl;R26RtdTomato/+(Control), PTHrP-creER;
PRRfl/+;R26RtdTomato/+(cHet) and PTHrP-creER;
PRRfl/fl;R26RtdTomato/+(cKO) mice(Takahashi et al., 2019 (link)).
(Fig S1). The DNA
extraction of mouse tail biopsies was done using a HotShot protocol, which
involved incubation of the tail sample at 95°C for 30 min in an alkaline
lysis reagent followed by neutralization. PCR-based genotyping was performed
using a standard protocol (GoTaq Green Master Mix, Promega, and Nexus X2,
Eppendorf). Mice were euthanized by overdosage of carbon dioxide or decapitation
under inhalation anesthesia in a drop jar (Fluriso, Isoflurane USP, VetOne).
Tokavanich N., Gupta A., Nagata M., Takahashi A., Matsushita Y., Yatabe M., Ruellas A., Cevidanes L., Maki K., Yamaguchi T., Ono N, & Ono W. (2019). A three-dimensional analysis of primary failure of eruption in humans and mice. Oral diseases, 26(2), 391-400.
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3

Genotyping of Transgenic Mouse Lines

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Osx-creER 6 , Hes1-creER 21 (link) and Cxcl12 GFP/+41 mice have been described previously. (Ono). All mice were housed in a specific pathogen-free condition, and analyzed in a mixed background. Mice were identified by micro-tattooing or ear tags. Tail biopsies of mice were lysed by a HotShot protocol (incubating the tail sample at 95 o C for 30 min in an alkaline lysis reagent followed by neutralization) and used for PCR-based genotyping (GoTaq Green Master Mix, Promega, and Nexus X2, Eppendorf). Perinatal mice were also genotyped fluorescently (BLS miner's lamp) whenever possible. Mice were euthanized by overdosage of carbon dioxide or decapitation under inhalation anesthesia in a drop jar (Fluriso, Isoflurane USP, VetOne).
Matsushita Y., Nagata M., Welch J.D., Wong S.Y., Ono W., & Ono N. (2020). Notch effector Hes1 marks an early perichondrial population of skeletal progenitor cells at the onset of endochondral bone development.
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