Liquid phenol

Amoxicillin trihydrate, triclosan (99%), and estrone (99+%) were purchased from Alfa Aesar (Ward Hill, MA, USA), while acetaminophen (≥98%) and atrazine (≥97%) were procured from Tokyo Chemical Industry (TCI, Chuo-ku, Tokyo, Japan). Liquid phenol, sodium nitroprusside dihydrate (≥98%), sodium hydroxide, sodium hypochlorite and ethanol were purchased from VWR (Radnor, PA, USA) and used as received with no further purification. HPLC grade acetonitrile from EMD Millipore (Bedford, MA, USA) and deionized (DI) water (Milli-Q, 18.2 MΩ cm) were employed as the mobile phase for HPLC analysis. Chemical oxygen demand (COD) kits were purchased from CHEMetrics (Midland, VA, USA) with a range of 0–1500 ppm. Nitrate nitrogen (NO₃-N) detection kits were purchased from HACH (Loveland, CO, USA).

Atrazine (≥97%) was purchased from Tokyo Chemical Industry (TCI) (Chuo-ku, Tokyo). Liquid phenol, sodium nitroprusside dihydrate (≥98%), sodium hydroxide, sodium hypochlorite, sodium bicarbonate, ammonium acetate, magnesium sulphate, calcium chloride, ammonium nitrate, and ethanol were purchased from VWR (Radnor, PA, USA) and used as received with no further purification. Monopotassium phosphate, dipotassium phosphate, zinc sulfate, manganese sulfate, magnesium sulfate, ferric chloride, and yeast extract were purchased from Sigma-Aldrich (St Louis, MO, USA). HPLC-grade acetonitrile from EMD Millipore (Bedford, MA, USA) and de-ionized (DI) water (Milli-Q, 18.2 MΩ cm) were employed as the mobile phase for HPLC analysis. Chemical oxygen demand (COD) kits were purchased from CHEMetrics (Midland, VA, USA) with a range of 0–1500 ppm. Total ammonia nitrogen (TAN) was determined using an established method [23 (link)] with ±0.02 mg/L accuracy. Nitrate nitrogen (NO₃-N) detection kits were purchased from HACH (Loveland, CO, USA).