Sterile 1 mm glass beads

Manufactured by Biospec

Sourced in United States

Sterile 1-mm glass beads are spherical particles made of high-quality glass with a diameter of 1 millimeter. They are designed for various laboratory applications that require small, uniform, and sterile solid objects.

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Lab products found in correlation

Proteinase inhibitor cocktail, by Roche (3 mentions) Sandwich elisa, by Hycult Biotech (2 mentions)

Close spelling variants of this product

Glass beads (110 citations) Sterile glass beads (4 citations)

8 protocols using sterile 1 mm glass beads

Quantifying Bacterial Growth in Experimental Endophthalmitis

Cited 4 times

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As previously described (73 (link), 89 (link)–91 (link), 93 (link), 94 (link), 96 (link)–98 (link)), eyes were harvested from euthanized mice at specific time points, homogenized in 400 μl PBS with sterile 1-mm glass beads (BioSpec Products, Inc., Bartlesville OK), serially diluted 10-fold in PBS, and plated onto BHI agar plates.
For in vivo bacterial growth analysis at different time points, experimental endophthalmitis was induced by intravitreally injecting approximately 100 CFU WT B. thuringiensis in 0.5 μl BHI into the right eyes of mice. At 4 h postinfection, one group of infected eyes was treated with OxPAPC, and another group served as the untreated control. At 2 h intervals thereafter, eyes were harvested for quantitation of intraocular bacterial growth (73 (link), 89 (link)–91 (link), 93 (link), 94 (link), 96 (link)–98 (link)).

Mursalin M.H., Coburn P.S., Livingston E., Miller F.C., Astley R., Flores-Mireles A.L, & Callegan M.C. (2020). Bacillus S-Layer-Mediated Innate Interactions During Endophthalmitis. Frontiers in Immunology, 11, 215.

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Quantifying Microbial Burden in Mouse Eyes

Cited 5 times

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As previously described, harvested eyes from euthanized mice at specific time points were homogenized in 400 µL PBS with sterile 1-mm glass beads (BioSpec Products, Inc., Bartlesville, OK, USA), serially diluted 10-fold in PBS, and plated onto BHI agar plates.²⁰ (link)^,²¹ (link)^,²⁴ (link)^,²⁵ (link)^,²⁸ (link)^,⁴⁴ (link)

Mursalin M.H., Coburn P.S., Miller F.C., Livingston E.T., Astley R, & Callegan M.C. (2021). C-X-C Chemokines Influence Intraocular Inflammation During Bacillus Endophthalmitis. Investigative Ophthalmology & Visual Science, 62(14), 14.

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Quantifying Intraocular Bacterial Loads

Cited 5 times

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Intraocular bacteria were quantified as previously described.1 (link),33 (link),34 (link),47 (link),48 (link),74 (link)–77 (link, no link found, link),83 (link) Briefly, infected eyes were harvested from euthanized mice at 0, 2, 6, 8, 10, and 12 hours postinfection. Infected eyes were then homogenized in 400 μL PBS with sterile 1-mm glass beads (BioSpec Products, Inc., Bartlesville, OK, USA). Eye homogenates were then diluted 10-fold in PBS, plated onto BHI agar plates and quantified by track dilution.

Mursalin M.H., Coburn P.S., Livingston E., Miller F.C., Astley R., Fouet A, & Callegan M.C. (2019). S-layer Impacts the Virulence of Bacillus in Endophthalmitis. Investigative Ophthalmology & Visual Science, 60(12), 3727-3739.

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Quantification of Intraocular Bacterial Load

Cited 3 times

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Intraocular bacteria were quantified as previously described (Moyer et al., 2009 (link), Novosad et al., 2011 (link), Parkunan et al, 2014 (link), Parkunan et al., 2015 (link), and Ramadan et al., 2008 (link)). Whole eyes were removed from euthanized mice at 3, 6, 9, and 12 h postinfection. Eyes were homogenized in PBS with sterile 1mm glass beads (BioSpec Products, Inc., Bartlesville, OK). Homogenates were plated and bacterial numbers were quantified by the track dilution method. Values represent the mean ± SEM for N≥6 eyes per time point. Two independent experiments were performed.

Callegan M.C., Parkunan S.M., Randall C.B., Coburn P.S., Miller F.C., LaGrow A.L., Astley R.A., Land C., Oh S.Y, & Schneewind O. (2017). The Role of Pili in Bacillus cereus Intraocular Infection. Experimental eye research, 159, 69-76.

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Quantifying Bacterial Infection in Eyes

Cited 1 time

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Bacteria were quantified by harvesting infected eyes at 0, 4, 8, and 12 hours postinfection. The eyes were homogenized with 1 mm sterile glass beads (Biospec products, Inc., Bartlesville OK) in 400 µl PBS. Bacteria were then track diluted 10-fold onto brain-heart infusion (BHI) agar and quantified [30] (link), [37] (link), [38] (link). Values represent the mean ± standard deviation (SD) for N≥4 eyes per time point.

Parkunan S.M., Astley R, & Callegan M.C. (2014). Role of TLR5 and Flagella in Bacillus Intraocular Infection. PLoS ONE, 9(6), e100543.

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Measuring Myeloperoxidase Levels in Ocular Inflammation

Cited 2 times

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Inflammatory cell influx was assessed by measuring myeloperoxidase concentrations using a sandwich ELISA (Hycult Biotech, Plymouth Meeting, PA, USA), as previously described. At various time points, eyes were harvested, transferred into PBS supplemented with proteinase inhibitor cocktail (Roche Diagnostics, Indianapolis, IN, USA), and homogenized using 1-mm sterile glass beads (BioSpec Products, Inc.). Uninfected eye homogenates were the negative controls. The lower limit of detection for this assay was 2 ng/mL.²⁴ (link)^,⁴² (link)

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Quantification of Ocular Inflammation

Cited 2 times

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As previously described, the extent of inflammatory cell influx into infected mouse eyes was semi-quantified using a myeloperoxidase (MPO) sandwich ELISA (Hycult Biotech, Plymouth Meeting PA). Infected eyes of euthanized mice were harvested at various time points and transferred into PBS supplemented with proteinase inhibitor cocktail (Roche Diagnostics, Indianapolis IN). Harvested eyes were then homogenized using 1-mm sterile glass beads (BioSpec Products, Inc.). Negative controls included homogenates of uninfected eyes (Mursalin et al., 2020b (link); Parkunan et al., 2014 (link)). The lower limit of detection for this assay was 2 ng/ml.

Mursalin M.H., Astley R., Coburn P.S., Miller F.C, & Callegan M.C. (2022). Roles of CCL2 and CCL3 in Intraocular Inflammation During Bacillus Endophthalmitis. Experimental eye research, 224, 109213.

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Quantifying Inflammatory Cell Infiltration via MPO ELISA

Cited 2 times

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Inflammatory cell infiltration was estimated by quantifying myeloperoxidase (MPO) using a sandwich ELISA (Hycult Biotech, Plymouth Meeting PA), as previously described. At 10 h postinfection, eyes were harvested, transferred into PBS supplemented with proteinase inhibitor cocktail (Roche Diagnostics, Indianapolis, IN) and homogenized using 1-mm sterile glass beads (BioSpec Products, Inc.). Uninfected eye homogenates were the negative controls. The lower limit of detection for this assay was 2 ng/ml (73 (link), 89 (link), 91 (link), 96 (link)–98 (link)).

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