Anti nemo

Manufactured by Cell Signaling Technology

Sourced in United States

Anti-NEMO is a primary antibody that recognizes the NEMO (NF-kappa-B essential modifier) protein. NEMO is a regulatory subunit of the IKK complex, which is essential for the activation of the NF-kappa-B signaling pathway. Anti-NEMO can be used to detect and study the NEMO protein in various experimental applications.

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Lab products found in correlation

2 protocols using anti nemo

Cellular Signaling Pathway Profiling

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Thrombin (T4648), all chemicals were purchased from Sigma-Aldrich Corp. (St. Louis, MO), unless otherwise indicated. Transfection was done with Lipofectamine 2000 (Invitrogen). Ni-NTA beads were purchased from Clontech (635660). Anti-MAVS (Cell signaling, #3993), anti-IRF3 (Santa Cruz, sc-9082), anti-Flag (Sigma, F3165), anti-HA (Sigma, H9658), anti-GAPDH (Santa Cruz, sc-25778), anti-TBK1 (Santa Cruz, sc-52957; Cell signaling, #3504), anti-IKKε (Cell signaling, #2690S), anti-NEMO (Cell signaling, #2686S), anti-His (cw00c28), anti-IκBα (Cell signaling, #4814), anti-p-IRF3 (Epitomics, 2562–1), anti-p-TBK1 (Abcam, ab109272), anti-p-IκBα (Cell signaling, #2859), anti-p-IKKα/β (Cell signaling, #2078), anti-IKKα (Cell signaling, #2682), anti-IKKβ (Cell signaling, #2370), anti-NAP1 (Proteintech, 15042-1-AP), anti-TANK (Bioworld, BS2231), anti-SINTBAD (Cell signaling, #8605) antibodies were purchased as indicated. Antisera against viperion and p54/56 were generated by immunizing mice with the full length recombinant protein produced in E. coli, at Beijing Biotop Biotechnology, China.

Fang R., Jiang Q., Zhou X., Wang C., Guan Y., Tao J., Xi J., Feng J.M, & Jiang Z. (2017). MAVS activates TBK1 and IKKε through TRAFs in NEMO dependent and independent manner. PLoS Pathogens, 13(11), e1006720.

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Antibody Validation for Protein Analysis

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Mouse anti-Myc (M47-3), anti-α-tubulin M175-3), anti-His (D291-3) and rabbit anti-β-actin were purchased from MBL (Nagoya, Japan); mouse anti-Flag (F3165) and anti-HA (H9658) from Sigma (St. Louis, MO, USA); and rabbit anti-IκBα (No.9242), anti-p-IκBα (No.2859), anti-p65 (No.4764), anti-USP7 (No.4833) and anti-NEMO (No.2685) from Cell Signaling Technology (Beverly, MA, USA). Recombinant TNFα and IL-1 were from PeproTech (Rocky Hill, NJ, USA). HSCARG was cloned into expression vectors pRK-HA, pRK-Flag and pcDNA-Myc-His, respectively, and verified by DNA sequencing. NEMO were cloned into pRK-HA or pRK-Flag expression vector. The shRNA plasmids were constructed by Shanghai Genechem Corporation (Shanghai, China). siRNA of NEMO (sc-29363) and IKKβ (sc-35644) were from Santa Cruz (Dallas, TX, USA). The sequences of the sense strand of USP7 shRNA was 5′-ACCCUUGGACAAUAUUCCU-3′ and 5′-AGUCGUUCAGUCGUCGUAU-3′.

Li T., Guan J., Li S., Zhang X, & Zheng X. (2014). HSCARG downregulates NF-κB signaling by interacting with USP7 and inhibiting NEMO ubiquitination. Cell Death & Disease, 5(5), e1229-.

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