Micromass q tof ultima

Manufactured by Waters Corporation

Sourced in United Kingdom, United States

The Micromass Q-Tof Ultima is a high-resolution mass spectrometer designed for advanced analytical applications. It utilizes quadrupole time-of-flight (Q-ToF) technology to provide accurate mass measurements and sensitive detection of a wide range of analytes.

Automatically generated - may contain errors

Lab products found in correlation

Synapt g2 hdms, by Waters Corporation (6 mentions) Bio spinr 6 tris column, by Bio-Rad (2 mentions) Illustra microspin g 25 column, by GE Healthcare (2 mentions) Bio spin 6 tris column, by Bio-Rad (1 mentions) C18 reverse phase column, by Macherey-Nagel (1 mentions) Q exactive hybrid quadrupole orbitrap, by Thermo Fisher Scientific (1 mentions) Avance 3 500 mhz high field nmr spectrometer, by Bruker (1 mentions) Ls6500 scintillation counter, by Beckman Coulter (1 mentions) Chemidoc mp, by Bio-Rad (1 mentions) Nap 5 column, by GE Healthcare (1 mentions)

8 protocols using micromass q tof ultima

Mass Spectrometry of Deglycosylated Human ST6Gal-I

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 5

Mass Spectrometry of Deglycosylated Human ST6Gal-I Enzymes

The molecular masses of variants of human ST6Gal-I expressed in HEK cells were analyzed. Delycosylated forms of human ST6Gal-I were analyzed using Micromass Q-Tof Ultima and Synapt G2 HDMS devices (Waters UK) and MassLynx V 4.1 software.

For deglycosylation samples of the sialyltransferase were denatured and reduced. To 100 μg sialyltransferase 45 μL denaturing buffer (6 M guanidinium hydrochloride) and 13 μL TCEP (=tris(2-carboxyethyl)phosphine; 0.1 mM, diluted in denaturing buffer) were added. Further the appropriate volume of ultrapure water was added, so that the overall concentration of guanidinium hydrochloride was about 4 M. After incubation of the sample for 1 h at 37° C. the buffer was changed using a Bio-Spin® 6 Tris column (Bio Rad), which was pre-equilibrated with ultrapure water. The whole sample was applied onto the column and eluted by centrifugation. To the resulting eluate 5.5 μL of 0.1 U/μL solution of PNGase-F was added and incubated at 37° C. over night. Afterwards the samples were adjusted to 30% ACN (=acetonitrile) and 1% FA (=formamide) and analyzed by electrospray ionization mass spectrometry.

US11078511B2. Aqueous composition (2021-08-03). Roche Diagnostics Operations, Inc. [US]. Inventors: Harald Sobek [DE], Michael Greif [DE], Marco Thomann [DE], Sebastian Malik [DE].

+ Open protocol

+ Expand

Mass Spectrometry Analysis of Deglycosylated Human ST6Gal-I

Check if the same lab product or an alternative is used in the 5 most similar protocols

The molecular masses of variants of human ST6Gal-I expressed in P.pastoris were analyzed by mass spectroscopy. Therefore, the deglycosylated forms of human ST6Gal-I were prepared and analyzed using Micromass Q-Tof Ultima and Synapt G2 HDMS devices (Waters UK) and the MassLynx V 4.1 software. For deglycosylation, samples were denatured and reduced; 45 μL denaturing buffer (6 M guanidine hydrochloride) and 13 μL TCEP (0.1 mM, diluted in denaturing buffer) were added to 100 μg sialyltransferase. An appropriate volume of ultrapure water was added to reach an overall concentration of about 4 M of guanidine hydrochloride. After incubation of the sample for 1 h at 37°C the buffer was changed using a Bio-SpinR 6 Tris column (BioRad), which was pre-equilibrated with ultrapure water. The whole sample was applied onto the column and eluted by centrifugation. To the resulting eluate, 5.5 μL of a 0.1 U/μL solution of PNGase F was added and the mixture was incubated at 37°C overnight. Afterwards the samples were adjusted to 30% ACN and 1% FA and analyzed by electrospray ionization mass spectrometry.

Ribitsch D., Zitzenbacher S., Augustin P., Schmölzer K., Czabany T., Luley-Goedl C., Thomann M., Jung C., Sobek H., Müller R., Nidetzky B, & Schwab H. (2014). High-quality production of human α-2,6-sialyltransferase in Pichia pastoris requires control over N-terminal truncations by host-inherent protease activities. Microbial Cell Factories, 13, 138.

+ Open protocol

+ Expand

Mass Spectrometry of Deglycosylated Human ST6Gal-I

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 5

Mass Spectrometry of Deglycosylated Human ST6Gal-I Enzymes

For deglycosylation samples of the sialyltransferase were denatured and reduced. To 100 μg sialyltransferase 45 μL denaturing buffer (6 M guanidinium hydrochloride) and 13 μL TCEP (=tris(2-carboxyethyl)phosphine; 0.1 mM, diluted in denaturing buffer) were added. Further the appropriate volume of ultrapure water was added, so that the overall concentration of guanidinium hydrochloride was about 4 M. After incubation of the sample for 1 h at 37° C. the buffer was changed using a Bio-SpinR 6 Tris column (Bio Rad), which was pre-equilibrated with ultrapure water. The whole sample was applied onto the column and eluted by centrifugation. To the resulting eluate 5.5 μL of 0.1 U/μL solution of PNGase-F was added and incubated at 37° C. over night. Afterwards the samples were adjusted to 30% ACN (=acetonitrile) and 1% FA (formamide) and analyzed by electrospray ionization mass spectrometry.

US11788108B2. CMP-dependent sialidase activity (2023-10-17). Roche Diagnostics Operations, Inc. [US]. Inventors: Harald Sobek [DE], Michael Greif [DE], Marco Thomann [DE], Sebastian Malik [DE].

+ Open protocol

+ Expand

Mass Spectrometry of Glycosylated ST6Gal-I

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 4

Mass Spectrometry of Glycosylated Human ST6Gal-I Enzymes

The molecular masses of variants of human ST6Gal-I expressed in HEK cells were analyzed. Glycosylated forms of human ST6Gal-I were prepared, and prepared material was analyzed using Micromass Q-Tof Ultima and Synapt G2 HDMS devices (Waters UK) and MassLynx V 4.1 software.

For mass spectrometry measurement the samples were buffered in electrospray medium (20% acetonitrile+1% formic acid). The buffer exchange was performed with Illustra™ MicroSpin™ G-25 columns (GE-Healthcare). 20 μg sialyltransferase variant with a concentration of 1 mg/mL was applied to the pre-equilibrated column and evaluated by centrifugation. The resulting eluate was analyzed by electrospray ionization mass spectrometry.

US11078511B2. Aqueous composition (2021-08-03). Roche Diagnostics Operations, Inc. [US]. Inventors: Harald Sobek [DE], Michael Greif [DE], Marco Thomann [DE], Sebastian Malik [DE].

+ Open protocol

+ Expand

Mass Spectrometry of Glycosylated ST6Gal-I

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 4

Mass Spectrometry of Glycosylated Human ST6Gal-I Enzymes

For mass spectrometry measurement the samples were buffered in electrospray medium (20% acetonitrile+1% formic acid). The buffer exchange was performed with Illustra™ MicroSpin™ G-25 columns (GE-Healthcare). 20 μg sialyltransferase variant with a concentration of 1 mg/mL was applied to the pre-equilibrated column and eluated by centrifugation. The resulting eluate was analyzed by electrospray ionization mass spectrometry.

US11788108B2. CMP-dependent sialidase activity (2023-10-17). Roche Diagnostics Operations, Inc. [US]. Inventors: Harald Sobek [DE], Michael Greif [DE], Marco Thomann [DE], Sebastian Malik [DE].

+ Open protocol

+ Expand

Comprehensive Analytical Methods for Novel Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

All reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA) or Fisher Scientific (Pittsburgh, PA, USA) and were of the highest purity commercially available. HPLC was performed on a Dionex Ultimate 3000 HPLC (Thermo Electron, Madison, WI, USA) system equipped with a diode array detector using Macherey-Nagel C18 reverse-phase column (Macherey-Nagel, Bethlehem, PA, USA). NMR spectra were acquired on a Bruker AVANCE III 500 MHz high-field NMR spectrometer (Bruker, Billerica, MA, USA) and the data were processed using Topspin software. Radiolabeled samples were counted in a Beckman LS6500 scintillation counter (Beckman, Indianapolis, IN, USA). HRMS spectra were acquired with either a Waters Micromass Q-tof Ultima (Waters, Milford, MA, USA) or a Thermo Scientific Q-Exactive hybrid Quadrupole Orbitrap (Thermo Scientific, San Jose, CA, USA). Fluorescence scanning was performed on a Biorad ChemiDoc MP (Biorad, Hercules, CA, USA) imaging system.

Curry A.M., Barton E., Kang W., Mongeluzi D.V, & Cen Y. (2020). Development of Second Generation Activity-Based Chemical Probes for Sirtuins. Molecules, 26(1), 11.

+ Open protocol

+ Expand

Sialylation of Humanized Monoclonal IgG1

Check if the same lab product or an alternative is used in the 5 most similar protocols

A highly galactosylated humanized monoclonal antibody IgG1 was used in sialylation experiments. The reaction mixture contained IgG1 (300 μg in 54 μL 35 mM sodium acetate/Tris buffer pH 7.0), the donor substrate CMP-Neu5Ac (150 μg in 50 μL water) and sialyltransferase (30 μg in 26 μL 20 mM potassium phosphate, 0.1 M NaCl, pH 6.5). The samples were incubated at 37°C for a defined time. To stop the reaction 100 μL denaturing buffer (6 M guanidine hydrochloride) and 30 μL TCEP (0.1 mM, diluted in denaturing buffer) were added to the samples and the samples were incubated at 37°C for 1 h. The samples were buffered in electrospray-medium (20% ACN, 1% FA) using pre-equilibrated illustraTM Nap5-Columns (GE-Healthcare). Samples were analyzed by electrospray ionization mass spectrometry and the content of G2 + 0SA, G2 + 1SA and G2 + 2SA N-glycans was determined. A Micromass Q-Tof Ultima and a Synapt G2 HDMS device (Waters UK) and the MassLynx V 4.1 software were used.

+ Open protocol

+ Expand

Mass Spectrometry of Labeled Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

Positive ion mass spectra of unlabeled and labeled proteins were acquired on a quadrupole time of flight mass spectrometer (Micromass Q-TOF Ultima; Waters, MA, USA) fitted with a Z-spray ionization source. Samples in phosphate-buffered saline (PBS; pH 7.4) were exchanged into deionized water containing 0.1% formic acid and made up to a final concentration of approximately 10 μM. The mass spectra were acquired with a capillary voltage of 2.6 kV, a cone voltage of 50 V, a source block temperature of 40 °C, and a resolution power of 5000 Hz. Cesium iodide was used for external calibration. Mass was calculated using MassLynx MS V4.1 (Waters, MA, USA).

Belfiore L., Spenkelink L.M., Ranson M., van Oijen A.M, & Vine K.L. (2018). Quantification of ligand density and stoichiometry on the surface of liposomes using single-molecule fluorescence imaging. Journal of controlled release : official journal of the Controlled Release Society, 278.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!