Cells were cultured for 48 h, collected, and rinsed twice with cold phosphate buffered saline (PBS). Cells (5 × 104) were suspended in 500 μl binding buffer. Annexin V-PE and 7-aminoactinomycin D (7AAD) (Annexin V-PE/7AAD apoptosis kit; Becton Dickinson and Co., NY, USA) were added according to the manufacturer's instructions. Flow cytometry was performed following calibration with an unstained cell sample and AnnexinV-FITC and PI-mono labelled samples. The proportion of apoptotic cells in each sample was calculated using FlowJo version 7.6 (FlowJo, LLC, Ashland, OR, USA).
Annexin 5 pe 7aad apoptosis kit
Manufactured by BD
Sourced in United States
The Annexin V-PE/7AAD apoptosis kit is a laboratory equipment used to detect and quantify apoptosis in cell samples. It utilizes two fluorescent dyes, Annexin V-PE and 7-Aminoactinomycin D (7-AAD), to identify cells undergoing early and late stages of apoptosis.
Automatically generated - may contain errors
Lab products found in correlation
Facscanto 2, by BD (2 mentions)
Facs caliber flow cytometer, by BD (2 mentions)
Facscalibur flow cytometer, by BD (2 mentions)
Annexin 5 pe, by BD (1 mentions)
Version 7, by FlowJo (1 mentions)
Facscanto 2 flow cytometer, by BD (1 mentions)
Live dead fixable near ir dead cell stain kit, by Thermo Fisher Scientific (1 mentions)
Facs caliber, by BD (1 mentions)
Facscan, by BD (1 mentions)
Modfit 3, by Verity Software House (1 mentions)
Fc400 mpl, by Beckman Coulter (1 mentions)
Hk 2 cell, by American Type Culture Collection (1 mentions)
Confocal microscope, by Leica (1 mentions)
Ros kit, by Beyotime (1 mentions)
Trizol, by Thermo Fisher Scientific (1 mentions)
Co2 incubator, by Sanyo (1 mentions)
Odyssey fc imager, by LI COR (1 mentions)
Mitosox reagent, by Merck Group (1 mentions)
Modfit software, by Verity Software House (1 mentions)
Propidium iodide rnase staining buffer, by BD (1 mentions)
18 protocols using annexin 5 pe 7aad apoptosis kit
1
Apoptosis Quantification by Flow Cytometry
2
Quantifying Apoptosis in PC Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
PC cells were transfected with siRNA as described above. 96 h post-transfection, both adherent and floating cells were collected and cell death was measured using the AnnexinV-PE/7-AAD reagent (Becton Dickinson AnnexinV-PE/7AAD Apoptosis Kit) according to the manufacturer's instructions. Samples were analysed on a BD FACSCanto-II flow cytometer.
3
Comprehensive Phenotypic Analysis of Dendritic Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The phenotypes of MDDCs, iDCs, and mature DCs (mDCs), together with the expression of markers related to the migration ability of DCs and T-cell proliferation, were assessed by flow cytometry using different combinations of mAbs (Figure S2 ). The corresponding isotypes were used as controls. The viability and mortality of all cell populations were assessed using an Annexin V-PE/7-AAD Apoptosis Kit (Becton Dickinson) or the LIVE/DEAD™ Fixable Near-IR Dead Cell Stain Kit (ThermoFisher Scientific, Massachusetts, USA). Samples were acquired by FACSCanto II (BD Biosciences; San Jose, CA, USA) and analyzed with FlowJo (Tree Star, Ashland, OR, USA).
4
Apoptosis Measurement by Flow Cytometry
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Apoptosis was assessed by cytometric analysis of Annexin V/7-AAD labeled cells using Annexin V-PE/7-AAD apoptosis kit (Becton Dickinson, Franklin Lakes, NJ, USA), according to manifacturer's protocol. Cell death was measured by propidium iodide staining; cells were incubated with 100ng/ml PI for 5 min before analysis. Cytometric analysis was performed with FACS-Canto II (Becton Dickinson).
5
Apoptosis Assay in GIST Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
GIST cells or transfected cells were treated with IM (IC50) for 48 h, stained using the Annexin V-PE/7AAD apoptosis kit (BD Biosciences) according to the manufacturer’s protocol. Data were acquired using a BD FACS Caliber flow cytometer and analyzed using BD Cell Quest software.
6
Apoptosis Evaluation by Flow Cytometry
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fixed cells were stained with the Annexin V-PE/7-AAD apoptosis kit (559763, BD Biosciences, Franklin Lakes, NJ) and apoptosis was evaluated by examining the percentage of apoptotic cells. Data acquisition and analysis were performed using Cell Quest software via a FACScan flow cytometer (BD Biosciences, Franklin Lakes, NJ). The results were analyzed with the ModFit 3.0 software (Verity Software House, Topsham, ME). All experiments were repeated in triplicate.
7
Cell Death Analysis by Flow Cytometry
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For cell death analysis, cells were stained using the Annexin V-PE/7-AAD Apoptosis Kit (BD Pharmingen) and then the dead cells were detected by flow cytometry (Beckman FC400 MPL, USA). The interpretation of the dot plot of Annexin V-PE/7-AAD stained cells analyzed by flow cytometry varies from paper to paper.
8
Annexin V-PE/7-AAD Apoptosis Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The apoptotic status was analysed by an Annexin V‐PE/7‐AAD Apoptosis Kit (559763; BD Biosciences, New Jersey, USA). Cells were stained and evaluated for apoptosis by flow cytometry according to the manufacturer's protocol. The number of apoptotic cells was analysed by flow cytometry (BD Biosciences, New Jersey, USA).
9
Investigating Apoptosis Pathways in HK-2 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
CO2 incubator (SANYO, Osaka, Japanese), transfer electrophoresis instrument (Liuyi Instrument Factory, Beijing, China); Odyssey FC imager (Li-COR Biosciences, Lincoln City, USA), cyclic variable temperature and heating PCR instrument (Bio-Rad company, Hercules, USA); confocal microscope (LEICA company, Wetzlar, Germany). HK-2 cells (American Type Culture Collection, Rockefeller, USA), 5Z-7-oxozeaenol (Millipore, Massachusetts, USA), SB203580 (APExBIO corporation, Houston, USA), FuGENE6 transfection reagent and TUNEL kit (Promega, Madison, USA), Annexin v-PE/7-AAD Apoptosis kit (BD corporation, New York, USA), ROS kit (Biyuntian Biotechnology, Shanghai, China), Rabbit anti p-TAK1 and t-TAK1 (Abcam, Cambridge, UK), Rabbit anti p38 MAPK and p-p38 MAPK (Cell Signaling, Boston, USA), Rabbit anti Bax, Bcl2 and TGF-β1 (proteintech, Chicago, USA), Horseradish Enzyme Labeled Goat Anti-Rabbit IgG and FITC-Goat Anti-Rabbit IgG (Zhongshan Jinqiao Company, Beijing, China), Trizol (Invitrogen, Carlsbad, USA), Reverse Transcription SysteM and deoxyribonucleoside triphosphate (dNTP) (Promega, Madison, USA), MitoSOX reagent (Sigma, Silicon valley, USA).
10
Apoptosis and Cell Cycle Analysis of Hepatocellular Carcinoma Cells
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!