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Nadp nadph quantification kit

Manufactured by Promega
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Sourced in United States

The NADP/NADPH Quantification Kit is a laboratory tool designed to measure the levels of NADP (Nicotinamide Adenine Dinucleotide Phosphate) and NADPH (Reduced Nicotinamide Adenine Dinucleotide Phosphate) in biological samples. It provides a colorimetric-based assay that allows for the quantification of these important cellular coenzymes.

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Lab products found in correlation

Mitochondria isolation kit, by Thermo Fisher Scientific (1 mentions) Succinate assay kit, by Abcam (1 mentions) Lactate colorimetric fluorometric assay kit, by Abcam (1 mentions) Hexokinase colorimetric assay kit, by Abcam (1 mentions)

Close spelling variants of this product

NADPH

2 protocols using nadp nadph quantification kit

1

Quantifying Metabolites and Cytokines

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The following kits were used according to the manufacturer’s instructions: Promega NADP/NADPH quantification kit (G9081) and NAD/NADPH quantification kit (G9071); Glyceraldehyde-3-Phosphate Dehydrogenase Activity Assay kit (abcam, ab204732); ELISAs for IL-1β (DY401), IL-6 (DY410), and TNFα (DY406).
De Jesus A., Keyhani-Nejad F., Pusec C.M., Goodman L., Geier J.A., Stoolman J.S., Stanczyk P.J., Nguyen T.A., Xu K., Suresh K.V., Chen Y., Rodriguez A.E., Shapiro J.S., Chang H.C., Chen C., Shah K.P., Ben-Sahra I., Layden B.T., Chandel N.S., Weinberg S.E, & Ardehali H. (2022). Hexokinase 1 cellular localization regulates the metabolic fate of glucose. Molecular cell, 82(7), 1261-1277.e9.
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2

Mitochondrial Metabolism Assessment

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Mitochondria were isolated by using the Mitochondria Isolation kit (#89874, Thermo, MA, USA) according to the manufacturer’s instructions. For hexokinase activity detection, mitochondria were isolated from cells and pellet was lysed and subjected to Hexokinase activity measurement by using Hexokinase Colorimetric assay kit (#K789-100, Biovision, CA, USA). G6PD activity measurement by using G6PD activity assay kit (#MAK015, Sigma-Aldrich, MO, USA). NADPH level performed using NADPH quantification kit (#V9510, Promega, WI, USA). NAD+/NADPH level were performed using NADP/NADPH quantification kit (#G9081, Promega, WI, USA). Sccinate levels were measured using a Succinate Assay Kit (#b204718, Abcam) and lactate Colorimetric/Fluorometric Assay kit (#K607-100, Biovision) according to the manufacturer’s protocol.
He Q.Q., Huang Y., Nie L., Ren S., Xu G., Deng F., Cheng Z., Zuo Q., Zhang L., Cai H., Wang Q., Wang F., Ren H., Yan H., Xu K., Zhou L., Lu M., Lu Z., Zhu Y, & Liu S. (2023). MAVS integrates glucose metabolism and RIG-I-like receptor signaling. Nature Communications, 14, 5343.
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