Benz(o)hydrazide (BH, 98%), phenylacetic hydrazide (PAH, 98%), benzoic acid, sodium hexachloroiridate(IV) hexahydrate (Na2IrCl6·6H2O, 99.9%), and 2,4-pyridinedicarboxylic acid (PDCA, ≥98%) were purchased from Sigma–Aldrich (Sigma Shanghai Branch, China). The purity of BH and PAH was checked by RP-HPLC and 1H-NMR spectroscopy, respectively, confirming a good purity. Acetic acid, sodium acetate, sodium dihydrogen phosphate, disodium hydrogen phosphate, trisodium phosphate, sodium bicarbonate, sodium carbonate, sodium chloride, hydrochloric acid, perchloric acid, sodium perchlorate, D2O and TSP were obtained either from Alfa Aesar or from Fisher Scientific (Alfa Aesar Shanghai Branch and Fisher Shanghai Branch, China); these chemicals were all of analytical grade and used without further purification. Methanol in HPLC grade and standard buffers of pH 4.00, 7.00, and 10.00 were also obtained from Fisher Scientific. Doubly distilled water was utilized to prepare all the solutions.
Sodium dihydrogen phosphate
Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom, India
Sodium dihydrogen phosphate is a chemical compound with the formula NaH2PO4. It is a white crystalline solid that is commonly used as a buffer in biochemical and analytical applications. The primary function of sodium dihydrogen phosphate is to maintain a specific pH level in solutions, which is essential for various laboratory procedures and experiments.
Automatically generated - may contain errors
Lab products found in correlation
Disodium hydrogen phosphate, by Thermo Fisher Scientific (8 mentions)
Potassium chloride (kcl), by Thermo Fisher Scientific (7 mentions)
Sodium chloride (nacl), by Thermo Fisher Scientific (5 mentions)
Sodium acetate, by Thermo Fisher Scientific (3 mentions)
Acetonitrile, by Thermo Fisher Scientific (3 mentions)
Sodium hydrogen phosphate, by Thermo Fisher Scientific (3 mentions)
Gallic acid, by Merck Group (3 mentions)
Hydrochloric acid (hcl), by Thermo Fisher Scientific (3 mentions)
Methanol, by Thermo Fisher Scientific (3 mentions)
Sodium hydroxide (naoh), by Thermo Fisher Scientific (3 mentions)
Sodium carbonate, by Thermo Fisher Scientific (2 mentions)
Acetic acid, by Thermo Fisher Scientific (2 mentions)
Sodium bicarbonate, by Thermo Fisher Scientific (2 mentions)
Ferric chloride, by Thermo Fisher Scientific (2 mentions)
Potassium persulfate, by Merck Group (2 mentions)
Formic acid, by Thermo Fisher Scientific (2 mentions)
Phosphoric acid, by Thermo Fisher Scientific (2 mentions)
Sucrose, by Thermo Fisher Scientific (2 mentions)
Ethylenediaminetetraacetic acid edta, by Merck Group (2 mentions)
Ms hu6, by GenScript (2 mentions)
20 protocols using sodium dihydrogen phosphate
1
Synthesis and Characterization of Metal Complexes
2
Synthesis of Bioactive Compounds
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Dimethylformamide, methanol, tetrahydrofuran, trimethylamine, ethyl chloroformate, dichloromethane, sodium dihydrogen phosphate, magnesium sulfate and acetonitrile were obtained from Fisher (Thermo Fisher Scientific, Strasbourg, Grand Est, France). Eicosapentaenoic acid and docosahexaenoic acid were obtained from Combiblocks (San Diego, CA, USA). 5-azacitidine was obtained from TCI chemicals (Tokyo, Kanto, Japan). Deionized water was obtained from a Milli-Q plus system (Merck-Millipore, Saint-Quentin-en-Yvelines, Île-de-France, France). Acetone and pyrene were obtained from Sigma-Aldrich (Saint-Quentin-Fallavier, Auvergne-Rhône-Alpes, France).
3
Formulation and Characterization of Amitriptyline Transdermal Delivery
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Amitriptyline hydrochloride (AMI-HCl), Brij™ O20, dichloromethane (DCM), anhydrous acetone, and sodium carbonate were obtained from Sigma-Aldrich (UK). Sodium dihydrogen phosphate, sodium hydrogen phosphate, magnesium sulphate, and dithiothreitol (DTT) were supplied by Fisher Scientific (UK). Phosphate-buffered saline (pH 7.3 ± 0.2 at 25 °C) was prepared using Dulbecco A tablets supplied by Oxoid (UK). Octyl salicylate (OSAL), PG, IPM, IPA, and 1-octanol were obtained from Sigma-Aldrich (UK). High-performance liquid chromatography (HPLC) grade water, HPLC grade acetonitrile, HPLC grade methanol, liquid chromatography/mass spectrometry (LC-MS) grade acetonitrile, LC-MS grade 0.1% trifluoroacetic acid (TFA) solution, limonene (LIM), ethyl acetate (EtAc), and TFA were supplied by Fisher Scientific (UK). Chloroform-d (CDCl3) were obtained from Cambridge Isotope Laboratories Inc. (UK). Propylene glycol monolaurate (PGML) Type II and TC were gifts from Gattefossé (St. Priest, France). All chemicals, unless otherwise stated, were ≥ 99% purity.
4
Solvent Evaluation for Phytochemical Analysis
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The solvents Petrol ether and Diethyl ether were obtained from Honeywell Riedel–de Haen. Methanol, Tween 40, Potassium hexacyanoferrate, Trichloroaceticacid (TCA), Linoleic acid, and Sodium chloride were purchased from PanReac AppliChem. n-Butanol, Gallic acid, 2,2-Di-Phenyl-1-Picryl-Hydrazyl (DPPH), Ascorbic acid, β-Caroten, Quercetin, Aluminum chloride AlCl3, and Ciocalteu’s phenol reagent were all bought from SIGMA ALDRICH. Disodium hydrogen phosphate, Sodium dihydrogen phosphate, and Ferric chloride were acquired from Fisher Scientific (S.A.). Ethanol was obtained from BIOSMART. Ethyl acetate and Dimethyl sulfoxide (DMSO) were purchased from LOBA Chemie. Sodium carbonate was acquired from HIMEDIA. The Muller-Hinton Agar and Potato Dextrose Agar mediums were purchased from Biokar Diagnostics (BK048 HA), France.
5
Phenol and Radical Scavenging Assays
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Folin-Ciocalteau phenol reagent, gallic acid, potassium persulfate and radical scavenging assay reagents: 6-hydroxy-2, 5, 7, 8-tetramethylchroman-2-carboxylic acid (Trolox), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2, 2′-azinobis-(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Sodium carbonate, methanol, hydrochloric acid (12 N), sodium chloride, sodium dihydrogen phosphate, sodium hydrogen phosphate, potassium chloride, sodium acetate, formic acid, acetonitrile and methanol (for HPLC grade) were supplied by Fisher Scientific.
6
Chitosan-Based Green Tea Nanoparticles
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Green tea leaves was purchased from Egyptian market. Chitosan (LMW 100 000–300 000 kDa), sodium tripolyphosphate, lecithin (soybean lecithin − 94% phosphatidylcholine), caffeine, catechin. epicatechin, and epigallocatechin gallate were purchased from Sigma-Aldrich Chemical Co. (St. Louis, USA). Acetic acid and Ethanol were supplied by Pio Chem, Cairo, Egypt. Sodium dihydrogen phosphate, sodium monohydrogen phosphate, acetonitirile, phosphoric acid and chloroform were purchased from Fisher Scientific (Loughborough, Leicestershire, UK). Cellophane membrane 12–14 kDa supplied by Spectrum Medical Inc, (Raleigh, North Carolina).
7
Murine Platelet Signaling Assays
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U46619 was from Cayman Chemical (Ann Arbor, MI). Thrombin receptor activating peptide 4 (TRAP4; AYPGKF-NH2) and ADP analog Adenosine 5′-[β-thio]diphosphate trilithium salt was from Sigma Aldrich (St. Louis, MO). Antibodies for Akt, pAkt, ERK and pERK were from Cell Signaling (Danvers, MA). ADP and other platelet disposables were from Chrono-Log (Havertown, PA). TRPC6 inhibitor (GsMTx-4) was from Alomone labs (Israel). FITC-conjugated Annexin V, anti–P-selectin, and PAC-1 antibodies were purchased from Cell Signaling Technology, Inc. (Danvers, MA). Fura-2 acetoxymethyl ester (fura-2/AM) and Pluronic F-127 were from Invitrogen (Grand Island, NY). Sodium citrate, whatman filter paper, ferric chloride, sodium chloride, potassium chloride, sodium dihydrogen phosphate, magnesium chloride, sodium bicarbonate, and D-dextrose were from Fisher Scientific (Hanover Park, IL). The radiolabeled [3H]SQ29,548 was purchased from pERKinElmer (Waltham, MA). BAPTA was purchased from Tocris Bioscience (Ellisville, Missouri).The C57BL/6 mice were obtained from Jackson laboratory (Bar Harbor, ME). Platelet count was determined using an automated hematology analyzer (Drew Scientific Dallas, TX).
8
Vancomycin Hydrochloride Analytical Protocol
9
Antioxidant Capacity Evaluation Methods
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Folin–Ciocalteau (FC) phenol reagent, gallic acid, potassium persulfate, and radical scavenging assay reagents (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and 2,2-azinobis-(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS)) were purchased from Sigma-Aldrich (St. Louis, MO, USA.). Sodium carbonate (SC), methanol, hydrochloric acid (HCl, 12 N), sodium chloride, sodium dihydrogen phosphate, sodium hydrogen phosphate, potassium chloride, sodium acetate, formic acid, acetonitrile, and methanol (MeOH HPLC grade) were supplied by Fisher Scientific (Loughborough, Leicestershire, UK).
10
Quantitative Analysis of Benzodiazepines
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4'-chlorodiazepam, alprazolam, clonazepam, desalkylflurazepam, diazepam, flunitrazepam, nitrazepam, oxazepam, prazepam and temazepam were obtained from Sigma-Aldrich (Dorset, UK). 3-hydroxyphenazepam, deschloroetizolam, diclazepam, etizolam, flubromazepam, flubromazolam, meclonazepam, phenazepam and pyrazolam were obtained from Chiron (Trondheim, Norway). All compounds were received as powdered solids.
Dimethyl sulfoxide (DMSO), methanol, phosphoric acid, sodium hydrogen phosphate heptahydrate, sodium dihydrogen phosphate, disodium hydrogen phosphate, acetic acid, sodium acetate trihydrate, boric acid, sodium hydroxide, hydrochloric acid, sodium chloride and octan-1-ol were purchased from Fisher Scientific (Leicestershire, UK). Phosphate buffered saline (PBS) tablets were purchased from Sigma-Aldrich (Dorset, UK).
Human plasma (pooled, from three male donors and three female donors) was obtained from Seralab (West Sussex, UK). Plasma was received frozen with sodium citrate as an anticoagulant.
Dimethyl sulfoxide (DMSO), methanol, phosphoric acid, sodium hydrogen phosphate heptahydrate, sodium dihydrogen phosphate, disodium hydrogen phosphate, acetic acid, sodium acetate trihydrate, boric acid, sodium hydroxide, hydrochloric acid, sodium chloride and octan-1-ol were purchased from Fisher Scientific (Leicestershire, UK). Phosphate buffered saline (PBS) tablets were purchased from Sigma-Aldrich (Dorset, UK).
Human plasma (pooled, from three male donors and three female donors) was obtained from Seralab (West Sussex, UK). Plasma was received frozen with sodium citrate as an anticoagulant.
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