Echomri 100 t

All of the animal studies were reviewed and approved by the Animal Welfare Committee (AWC) of University of Texas Health Science Center at Houston (Animal protocol number: AWC-21-0019). Wild-type (WT) C57BL/6J (Stock 000664) and Adiponectin-Cre (Stock 010803) mice were purchased from Jackson Laboratories. The Ces1d-floxed (Ces1d^flx/flx) mouse was generated by Dr. Grant A Mitchell from the Ste-Justine Hospital in Montreal, Canada, and crossed onto C57BL/6J background (18 (link)). 8-wk-old male FKO and their littermate floxed control (from here on referred to as WT) mice were used for all the experiments. Mice were housed in the animal facility (22°C ± 1°C) on a 12-h light/dark cycle with ad libitum access to water and regular chow diet, unless indicated otherwise. For the HFD challenge, the age matched male WT and FKO mice were fed with the HFD (containing 60% calories from fat; Bio-Serv) for 14 or 25 wk. The total body mass, fat mass and lean mass were measured by EchoMRI-100T (Echo Medical Systems).