P lyn y507

Manufactured by Cell Signaling Technology

P-LYN (Y507) is a primary antibody that specifically recognizes the phosphorylated form of the LYN protein at tyrosine 507. LYN is a member of the Src family of non-receptor tyrosine kinases that play a role in various cellular processes.

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P-Lyn (9 citations)

2 protocols using p lyn y507

Western Blot Analysis of BCR Signaling

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Luxeptinib was provided by Aptose Biosciences. Ibrutinib (#HY-10997) was purchased from MedChem Express. Goat anti-human IgM (#109-005-043) was purchased from Jackson ImmunoResearch. Human lymphoma cell lines SU-DHL-6 (#CRL-2959), JeKo-1 (#CRL-3006), RL (#CRL-2261) and Fetal Bovine Serum (#30–2020) were obtained from ATCC. SU-DHL-6 is a human lymphoblast-like cell line, JeKo-1 is a mantle cell lymphoma cell line and RL is a human non-Hodgkin’s lymphoma B cell line. RPMI-1640 medium (#11875–093) and penicillin/streptomycin (#15070063) were purchased from Thermo Fisher Scientific. Antibodies against p-BTK (Y223) (#87141), BTK (#56044), p-PLCγ2 (#3871), p-SYK (#2710), SYK (#80460), p-BLNK (#3601), BLNK (#36438), p-CD79A (#5173), p-LYN/LCK/HCK/BLK (#70926), p-LYN (Y507) (#2731), LYN (#2796, #4576), pSrc family (#6943) and GAPDH (#2118) were purchased from Cell Signaling Technology. Phospho-BTK (Y551) (#ab40770) was purchased from Abcam. PLCγ2 (#sc-5283) and CD79A (#sc-20064) were purchased from Santa Cruz Biotechnology. All other reagents and chemicals used were of analytical grade and were obtained from Sigma-Aldrich or Thermo Fisher Scientific.

Sonowal H., Rice W.G, & Howell S.B. (2023). Luxeptinib interferes with LYN-mediated activation of SYK and modulates BCR signaling in lymphoma. PLOS ONE, 18(3), e0277003.

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Western Blot Analysis of Signaling Proteins

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Cells were harvested and disrupted in a radioimmunoprecipitation assay (RIPA) lysis buffer buffer (50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 0.1% SDS, 1% NP-40, 0.25% Sodium deoxycholate and 1 mM EDTA) with protease inhibitor cocktail, 1 mM NaF and 1 mM Na₃VO₄. Equal amounts (~20 μg) of whole cell lysates were resolved on a 10% SDS-PAGE gel (Bio-Rad). After electrophoresis, samples were electrotransferred to a nitrocellulose membrane (Bio-Rad), probed with relevant primary antibodies at 4 °C overnight, incubated with HRP (horseradish peroxidase) conjugated secondary antibody (Jackson ImmunoResearch, West Grove, PA), and detected with an enhanced chemiluminescence substrate (Amersham, Piscataway, NJ). The following primary antibodies were used: p-Src (Y416), total Src, p-Lyn (Y507), total Lyn, p-Fyn (Y530) and Fyn (Cell Signaling, Danvers, MA); HIF-1α (Genetex, Irvine, CA); β-actin (Sigma). To quantify the band intensity,

Dai Y, & Siemann D. (2019). c-Src is required for hypoxia-induced metastasis-associated functions in prostate cancer cells. OncoTargets and therapy, 12, 3519-3529.

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