All chemicals were of analytical grade and used directly as received from the manufacturers without additional purification. Zinc chloride (ZnCl2, purity >99.5, Lach-Ner, Neratovice, Czech Republic) was used as a zinc source, sodium hydroxide (NaOH, purity >98%, Carlo Erba Reagents) as a precipitating agent, cetyltrimethylammonium bromide (CTAB, C19H42BrN, Alfa Aesar, Ward Hill, MA, USA) as a cationic surfactant and triblock copolymer Pluronic F127 (EO106PO70EO106, Sigma-Aldrich) was employed as a nonionic surfactant. Distilled water was used as the solvent and for powder rinsing, while absolute ethanol (C2H5OH, 96%, Zorka Pharma, Serbia) was used for final rinsing.
Pluronic f127 eo106po70eo106
Manufactured by Merck Group
Sourced in United States
Pluronic F127 is a non-ionic triblock copolymer surfactant consisting of a central hydrophobic block of polypropylene oxide (PO) flanked by two hydrophilic blocks of polyethylene oxide (EO). It has an average molecular weight of approximately 12,600 Daltons.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions)
Sodium hydroxide (naoh), by Carlo Erba (1 mentions)
Anhydrous glycerol, by Merck Group (1 mentions)
Imidazole, by Merck Group (1 mentions)
Pepsin from porcine gastric mucosa, by Merck Group (1 mentions)
K2hpo4, by Merck Group (1 mentions)
Sodium chloride (nacl), by Merck Group (1 mentions)
Tetraethyl orthosilicate teos, by Merck Group (1 mentions)
Anti cd4 fitc, by BioLegend (1 mentions)
Anti cd8 pe cy7, by BioLegend (1 mentions)
Anti cd206 pe, by BioLegend (1 mentions)
Anti cd45 pe, by BioLegend (1 mentions)
Anti cd11b apc, by BioLegend (1 mentions)
Calcium colorimetric assay kit, by Beyotime (1 mentions)
Fitc anti cd80, by BioLegend (1 mentions)
Anti cd3 apc, by BioLegend (1 mentions)
Cell counting kit 8 cck 8, by Beyotime (1 mentions)
Annexin 5 fitc apoptosis detection kit, by Beyotime (1 mentions)
8 protocols using pluronic f127 eo106po70eo106
1
Synthesis of Zinc Oxide Nanoparticles
2
Synthesis and Characterization of Mesoporous Silica Nanoparticles
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N,N-dimethylhexadecylamine (DHMA, ≥95%, Sigma Aldrich, Saint Louis, MO, USA), Pluronic F127 (EO106PO70EO106, Mw = 12600, Sigma Aldrich), cetyltrimelammonium bromide (CTAB, ≥95%, Sigma Aldrich), NaOH (Macron Fine Chemicals, Radnor, PA, USA), tetraethyl orthosilicate (TEOS, ≥99%, Sigma Aldrich), anhydrous glycerol (J.T. Baker, Radnor, PA, USA), glucose (≥99.5%, Sigma Aldrich), imidazole (99%, Sigma Aldrich), Tris base (Tris, ≥99.8%, J.T. Baker), KH2PO4 (≥95%, High Purity, Santiago de Querétaro, QRO, Mexico), K2HPO4 (≥98%, Sigma Aldrich), NaCl (≥99%, J.T. Baker), pepsin from porcine gastric mucosa (Sigma Aldrich), yeast extract (DIBICO, Cuautitlán Izcalli, EDOMÉX, Mexico) and casein peptone (DIBICO). For the synthesis of MSN, deionized water with a resistivity of 18.2 MΩ•cm was used. For all other experiments double distilled water was employed.
3
Pluronic F127 and Dopamine Hydrogel Synthesis
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Triblock poly (ethylene oxide)-bpoly(propylene oxide)-b-poly(ethylene oxide) Pluronic F127 (EO106PO70EO106, Mav = 12,600) and dopamine hydrochloride (C8H11NO2·HCl, DA·HCl) were brought from Sigma-Aldrich. Ethanol (C2H6O) was purchased from Sinopharm Chemical Reagent Co., Ltd. 1,3,5-trimethylbenzene (C9H12), Calcium chloride, hydrogen peroxide (H2O2, 30 wt% solution in water), ammonia water (25–28%), N-2-hydroxyethyl piperazine-N′-2-ethanesulfonic acid (HEPES), Hyaluronic acid sodium salt from Streptococcus equi, Rhodamine B (RhB) and dichloride [Ru(dpp)3]Cl2 were purchased from Aladdin (Shanghai, China). The lactate assay kit was brought from Nanjing Jiancheng Bioengineering Institute. Cell Counting Kit-8 (CCK-8), the Annexin V-FITC Apoptosis Detection Kit, and the Calcium Colorimetric Assay Kit were obtained from Beyotime Biotechnology Co., Ltd. Anti-CD16/32, anti-CD45-PE, anti-CD11b-APC, anti-CD11c-APC anti-F4/80-PE/Cy7, anti-CD80-FITC, anti-CD206-PE, anti-CD86-PE, anti-MHCII-V450, anti-CD3-APC, anti-CD4-FITC, and anti-CD8-PE/Cy7 were purchased from BioLegend. ELISA kits were purchased from Dakewei Biotech Co., Ltd.
All chemicals were of analytical grade and used without further purification. Deionized water (18.2 MΩ·cm resistivity at 25 °C) was used for all experiments.
All chemicals were of analytical grade and used without further purification. Deionized water (18.2 MΩ·cm resistivity at 25 °C) was used for all experiments.
4
Facile Synthesis of Graphene-Zirconia Nanocomposite
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All chemical reagents of analytical grade were used without further purification. Graphite powder flakes (>99.99 wt%), zirconium(iv ) propoxide (C12H28O4Zr, 70 wt% in 1- propanol), and Pluronic F127 (EO106PO70EO106) were procured from Sigma-Aldrich; InCl3 indium(iii ) chloride, urea, SDBS – sodium dodecyl benzene sulphonate, and tetraethyl orthosilicate (TEOS, Acros Organics) were procured from Samchun Pure Chemical Co Ltd. Ethylene glycol (C2H6O2, 99.5%) was purchased from Duksan Pure Chemicals Co. Ltd., Korea; hydrochloric acid (HCl) was purchased from Dae-Jung Chemical Co. Ltd., Korea. Deionized water (18.2 MΩ cm−1) was used in all the experiments.
5
BMP-2 Functionalized Silica Hydrogel
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3-Aminopropyltrimethoxysilane (APTMS) and glutaraldehyde were purchased from Aladdin (USA). Desferoxamine (DFO) and dibenzocyclooctyne-amine (DBCO-NH2) were purchased from Sigma (USA). PEG (Mw = 600 g/mol), succinic anhydride, N, N′-diisopropylcarbodiimide (DIC) and N-hydroxy succinimide (NHS) were purchased from Aladdin (USA). 3-azido-1-propylamine (Az-NH2) was purchased from TCI (USA). Recombinant human BMP-2 was obtained from Shanghai Rebone Biomaterials Co. Ltd. (Shanghai, China). Tetraethyl orthosilicate (TEOS), Ca (NO3)2·4H2O and triethyl phosphate (TEP) were obtained from Shanghai Ling Feng Chemical Reagent Co., Ltd. (Shanghai, China). A human BMP-2 ELISA kit, tetracycline hydrochloride and Pluronic®F-127 (EO106PO70EO106) were purchased from Sigma–Aldrich (USA).
All experimental animals were obtained from Shanghai Jiao Tong University School of Agriculture and Biology. The animal experiments were performed in strict accordance with the NIH Guide for the Care and Use of Laboratory Animals, and all procedures were carried out by the Animal Research Committee of Sixth People's Hospital, Shanghai Jiao Tong University School of Medicine (License number: SCXK (Hu) 2017-0007, SYXK (Hu) 2018-0006; Animal research ethics number: NO. DWLL20220602)
All experimental animals were obtained from Shanghai Jiao Tong University School of Agriculture and Biology. The animal experiments were performed in strict accordance with the NIH Guide for the Care and Use of Laboratory Animals, and all procedures were carried out by the Animal Research Committee of Sixth People's Hospital, Shanghai Jiao Tong University School of Medicine (License number: SCXK (Hu) 2017-0007, SYXK (Hu) 2018-0006; Animal research ethics number: NO. DWLL20220602)
6
Synthesis of Multifunctional Polymer Composite
7
Mesoporous Bioactive Glass Scaffold Fabrication
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α-MEM culture medium, Fetal bovine serum (FBS), Penicillin-Streptomycin and phosphate buffered saline (PBS) were from Gibco® (Thermo Fisher Scientific Inc. MA, USA). Pluronic® F-127 (EO106PO70EO106), fibrinogen, thrombin, fluorescein isothiocyanate-phalloidin (FITC-phalloidin) and DAPI were from Sigma-Aldrich (St. Louis, MO, USA). Cell Counting Kit-8 (CCK-8) was purchased from Dojindo (Shanghai, China). BCA protein assay kit and BCIP/NBT alkaline phosphatase color development kit were from Beyotime Biotech (Jiangsu, China). RNAiso Plus, PrimeScript RT reagent kit and TB Green™ Premix Ex Taq™ were from Takara (Tokyo, Japan). Chemical reagents used for MBG synthesis including tetraethyl orthosilicate (TEOS), Ca(NO3)2·4H2O and triethyl phosphate (TEP) were from Sinopharm Group Co. Ltd. (Shanghai, China). Histological reagents including 4% paraformaldehyde and 10% EDTA (0.1 M, pH 7.1) solution were from Servicebio Technology (Wuhan, China).
8
Synthesis and Functionalization of Silica Nanoparticles
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2-(N-morpholino)ethanesulfonic acid (MES, Sigma-Adrich, Darmstadt, Germany), 3-aminopropyltriethoxysilane (APTES, Sigma-Adrich), 3-mercaptopropyl triethoxysilane (MPTES, >95%, Sigma-Adrich), ammonium fluoride (NH4F, >98% Fluka, Darmstadt, Germany), Atto 633-carboxy dye (Atto-Tec, Siegen, Germany), 4′,6-diamidino-2-phenylindole (DAPI, Thermo Fisher, Schwerte, Germany), block copolymer surfactant (Pluronic F127 (EO106PO70EO106), Sigma-Aldrich), cetyltrimethyl-ammonium chloride (CTAC, 25% in H2O, Sigma-Adrich), N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC, >97%, Sigma-Adrich), N-hydroxysulfosuccinimide (sulfo-NHS, 98%, Sigma-Adrich), octadecyltrimethylammonium bromide (C18Br, Sigma-Adrich), propidium iodide, tetraethylorthosilicate (TEOS, >98%, Sigma-Aldrich), triethanolamine (TEA, 98%, Fluka), triisopropylbenzene (TiPB, 96%, Sigma-Adrich). Oligomer 454 [42 (link)] and Mal-PEG-GE11 [41 (link)] were synthesized as described before. siRNA and miRNA duplexes were purchased from Axolabs GmbH (Kulmbach, Germany): control RNA (sense: 5-AuGuAuuGGccuGuAuuAGdTsdT-3′, antisense: 5′-CuAAuAcAGGCcAAuAcAUdTsdT-3), miR200c (sense: 5′-UCCAUCAUUACCCGGCAGUAUUA-3, antisense: 5′-UAAUACUGCCGGGUAAUGAUGGA-3).
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