Sri 13k

Manufactured by Merck Group

Sourced in United States

The SRI-13K is a lab equipment product manufactured by Merck Group. It is a specialized device used for various laboratory tasks. The core function of the SRI-13K is to perform precise measurements and analyses within a controlled environment.

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Lab products found in correlation

Nefa hr kit, by Fujifilm (2 mentions) Ml 82k, by Merck Group (2 mentions) Sandwich elisa, by Mercodia (1 mentions) 1470 automatic gamma counter, by PerkinElmer (1 mentions) Cobas integra 400, by Roche (1 mentions)

7 protocols using sri 13k

Biochemical Profiling of Metabolic Markers

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Enzymatic colorimetric methods were used to measure total cholesterol [(Kit No A11A01634) from Horiba ABX, France], plasma triglycerides [(Kit No 12146029 triglycerides) from Roche Diagnostics GmbH, Germany] as well as triglycerides in the liver [(Kit No A11A01640) from Horiba ABX, France] following homogenization in isopropanol.
Plasma insulin level was measured with a radioimmunoassay (SRI-13K, Millipore Corporation, USA) on a 1470 Automatic Gamma Counter (PerkinElmer, USA). Glucagon level was measured using sandwich ELISA from Mercodia (Mercodia A/S, Uppsala, Sweden). Alanine aminotransferase (ALAT) was measured using the optimized UV-test according to IFCC (International Federation of Clinical Chemistry) modified method without pyridoxal phosphate (ABX Pentra 400, USA). Beta hydroxybutyrate (nmol/well) was measured using colorimetric probe with an absorbance band at 450 nm (Abcam, USA). N-terminal prohormone of brain natriuretic peptide (NTpro BNP) was measured using sandwich ELISA (Kamiya Biomedical Company, USA).

Adingupu D.D., Göpel S.O., Grönros J., Behrendt M., Sotak M., Miliotis T., Dahlqvist U., Gan L.M, & Jönsson-Rylander A.C. (2019). SGLT2 inhibition with empagliflozin improves coronary microvascular function and cardiac contractility in prediabetic ob/ob−/− mice. Cardiovascular Diabetology, 18, 16.

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Fasting Mice Insulin and NEFA Levels

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Tissues and sera were collected from sacrificed mice after overnight fasting (18:00 – 08:00). Insulin level was measured using a RIA kits (Millipore Co. SRI-13K, ML-82K). The content of Non-Esterified Fatty Acids (NEFA) was measured using a NEFA-HR Kit (Wako), respectively.

Kim T., He L., Johnson M.S., Li Y., Zeng L., Ding Y., Long Q., Moore J.F., Sharer J.D., Nagy T.R., Young M.E., Wood P.A, & Yang Q. (2014). Carnitine Palmitoyltransferase 1b Deficiency Protects Mice from Diet-Induced Insulin Resistance. Journal of diabetes & metabolism, 5(4), 361-.

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Insulin Measurement in Rat Tissue

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Animals were anesthetized with sodium pentobarbital and serum and brains were collected. The left hemi brain was frozen on dry ice and stored at −70. The right hemi brain was minced and homogenized in 5% TFA, 80% EtOH, and centrifuged for 10 min at 5000xg at 4C. Three ml of the supernatant were collected and lyophilized. The samples were reconstituted in 0.6 ml of PBS, and insulin was measured using the sensitive rat insulin RIA kit (Millipore #SRI-13K). For serum, 0.1 ml was submitted to RIA. In a separate group of rats, CSF was collected from the posterior fossa and was pooled until 200 μl was achieved. Matching plasma from the same animals was pooled in the same proportions. These CSF and serum samples were then assayed by RIA. Mean values were compared by Student’s t-test.

Shi Z., Hansen K.M., Bullock K.M., Morofuji Y., Banks W.A, & Brooks V.L. (2019). Resistance to the sympathoexcitatory effects of insulin and leptin in late pregnant rats. The Journal of physiology, 597(15), 4087-4100.

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Oral Glucose Tolerance Test in Rats

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An OGTT was performed 2 weeks before the sacrifice. Food was removed from rats 18 h before they were given orally a glucose dose (1 g glucose/kg body weight, between 08.00 and 10.00 am). Blood samples were collected from the tail vein in heparinized tubes immediately before glucose administration to determine the basal glucose and insulin values and 5, 25, 40, 60 and 180 min after. Glucose values were determined with a glucose analyzer (ACCU-CHECK Active, Softclix). After centrifugation (3000 × g, 7 min, 4°C) plasma samples were stored at −20°C until insulin determination using a radioimmunoassay kit (SRI-13 K, Millipore, Molheim, France). The total area under the curve (AUC) for glucose was then calculated in order to evaluate the glucose tolerance as previously used by Cortez et al.
[21 (link)].

Mourmoura E., Chaté V., Couturier K., Laillet B., Vial G., Rigaudiere J.P., Morio B., Malpuech-Brugère C., Azarnoush K, & Demaison L. (2014). Body adiposity dictates different mechanisms of increased coronary reactivity related to improved in vivo cardiac function. Cardiovascular Diabetology, 13, 54.

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Glucose and Insulin Measurement in Mice

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Fasting blood glucose and insulin levels were measured in mice at 19 weeks of age, except for the NZO males which were measured at 12 weeks of age. Glucose was analyzed by the glucose oxidase method using a commercially available kit (TR15221, Thermo Fisher Scientific), and insulin was measured by radioimmunoassay (RIA; SRI-13K, Millipore). This is the same assay that was used to measure plasma insulin for the previously published cohort used for the correlation analysis in Figure 4; Mitok et al., 2018 (link).

Emfinger C.H., Clark L.E., Yandell B., Schueler K.L., Simonett S.P., Stapleton D.S., Mitok K.A., Merrins M.J., Keller M.P, & Attie A.D. (2023). Novel regulators of islet function identified from genetic variation in mouse islet Ca2+ oscillations. eLife, 12, RP88189.

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Fasting Blood Hormone Measurements in Male Mice

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For blood hormone measurements, male mice were fasted overnight (14 h), and a blood sample was obtained by cardiac puncture immediately prior to sacrifice, serum was immediately separated by centrifugation (4,000 rpm for 10 m at 4°C) and frozen at -80°C until further analysis. Fasting serum glucose, cholesterol, triglycerides, non-esterified fatty acids (NEFAC test) and HDL cholesterol (HDLC3) were measured in duplicates on a COBAS INTEGRA 400 (Roche Diagnostics, Basel, Switzerland). Fasting serum insulin and leptin levels were measured in duplicates by Sensitive Rat Insulin radioimmunoassay (RIA; Catalogue # SRI 13K) and Mouse Leptin RIA, respectively (XL 85K; Millipore Corporation, Billerica, MA, USA).

Fullston T., Ohlsson-Teague E.M., Print C.G., Sandeman L.Y, & Lane M. (2016). Sperm microRNA Content Is Altered in a Mouse Model of Male Obesity, but the Same Suite of microRNAs Are Not Altered in Offspring’s Sperm. PLoS ONE, 11(11), e0166076.

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Fasting Mice Insulin and NEFA Levels

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