Icatibant (peptide kinin B2 receptor antagonist) and des-Arg9-[Leu8]-bradykinin (DALBk; peptide kinin B1 receptor antagonist) were obtained from Sigma Chemical Company (St. Louis, MO, USA). FR173657 (non-peptide kinin B2 receptor antagonist) and SSR240612 (non-peptide kinin B1 receptor antagonist) were obtained from Sanofi-Aventis (Germany). Aprotinin was acquired from Sigma-Aldrich Chemical Company (St. Louis, MO, USA). Kinin receptor antagonists and Aprotinin were prepared in a saline solution (0.9% NaCl), which was used as the vehicle in control groups. The enzyme immunoassay kit for bradykinin was obtained from Peninsula Laboratories International, Inc. (San Carlos, CA, USA). The doses of the drugs used in this study were based on pilot experiments or previous studies. They were administered in a volume of 10 mL/kg (systemic administration) or 20 μL/paw (intraplantar injection) [22 (link),23 (link)].
Icatibant
Manufactured by Merck Group
Sourced in United States
Icatibant is a synthetic peptide that functions as a bradykinin B2 receptor antagonist. It is primarily used in the treatment of acute attacks of hereditary angioedema.
Automatically generated - may contain errors
Lab products found in correlation
Des arg9 leu8 bradykinin dalbk, by Merck Group (2 mentions)
Fr173657, by Sanofi (2 mentions)
Ssr240612, by Sanofi (2 mentions)
Aprotinin, by Merck Group (1 mentions)
Bradykinin, by Merck Group (1 mentions)
Des arg9 bradykinin, by Merck Group (1 mentions)
Cisplatin, by Merck Group (1 mentions)
Calphostin c, by Merck Group (1 mentions)
L name, by Merck Group (1 mentions)
M 1 cell line, by American Type Culture Collection (1 mentions)
Letrozole, by Bio-Techne (1 mentions)
Annexin 5, by Thermo Fisher Scientific (1 mentions)
Anastrozole, by Bio-Techne (1 mentions)
Paclitaxel, by Merck Group (1 mentions)
7 aminoactinomycin d 7 aad, by BD (1 mentions)
4 protocols using icatibant
1
Kinin Receptor Antagonists in Inflammation
2
Kinin Receptor Modulators in Cisplatin Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cisplatin (cis-diamminedichloridoplatinum II, C-Platin®; Blau, SP, Brazil), bradykinin (Bk; kinin B2 receptor agonist), Icatibant (peptide kinin B2 receptor antagonist), des-Arg9-bradykinin (DABk; kinin B1 receptor agonist), and des-Arg9-[Leu8]-bradykinin (DALBK; peptide kinin B1 receptor antagonist) were purchased from Sigma Chemical Company (St. Louis, MO, USA). FR173657 (non-peptide kinin B2 receptor antagonist) and SSR240612 (non-peptide kinin B1 receptor antagonist) were obtained from Sanofi-Aventis (Berlin, Germany). Antisense oligonucleotides targeting the kinin B1 receptor (5′-AGG TTC CTG TGG ATG GCG TCCC-3′), kinin B2 receptor (5′-AGA ATT CTG TTC ACT GTT TCT TCC CTG-3′), and nonsense oligonucleotides (5′-GGT GGA T TTG AGG ATT TCG GC-3′) were acquired from GenOne Biotechnologies (Rio de Janeiro, Brazil). Cisplatin and antagonists were prepared in a saline solution (0.9%). Phosphate-buffered saline (PBS; 10 mM) was used to dilute reagents administered via the intraplantar route (kinin B1 and B2 receptor agonists). The control groups (vehicles) received the vehicles where the treatments were solubilized. All the intraperitoneal treatments were administered in mice in a volume of 10 mL/kg, while intraplantar injections were administered in a volume of 20 µL.
3
Bradykinin Signaling Pathway Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Bradykinin (B3259), H89 (PKA inhibitor, B1427), calphostin C (PKC inhibitor, C6303), L‐NAME (NOS inhibitor, N5751), Icatibant (HOE 140 B2R antagonist, H157) were purchased from Sigma‐Aldrich (Saint Louis, MO). For detection of prorenin and renin, we used a rabbit anti‐renin polyclonal IgG H‐105 antibody (sc‐22752), B2R was detected using anti‐B2R goat polyclonal IgG antibody (sc‐15050) and β‐actin was detected using a mouse anti‐β‐actin monoclonal IgG antibody (sc‐4778) all from Santa Cruz Biotechnology, Santa Cruz, CA. Anti‐rat aquaporin‐2 (AQP2) antibody was purchased from Abcam (Cambridge, UK). For Western blot experiments the secondary antibodies used were the IR Dye 800CW or 650 anti‐goat, mouse and rabbit according to the primary antibody chosen (Li‐Cor Bioscience, NE) and for immunofluorescence the secondary Alexa fluor antibodies (Alexa fluor‐488 or ‐594) were purchased from Life Technologies (Carlsbad, CA). M‐1 cell line was obtained from American Type Culture Collection (ATCC, CRL‐2038, Manassas, VA).
4
Pharmacological Modulation of Bradykinin Receptors
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Anastrozole and letrozole were purchased from Tocris Bioscience (Bristol, UK) and were diluted in 0.5% carboxymethylcellulose and 99.5% of NaCl (0.9%). Paclitaxel (6 mg/mL of Paclitaxel in Cremophor EL and dehydrated ethanol) was purchased from Glenmark (Buenos Aires, ARG) and was dissolved in NaCl (0.9%). Icatibant (kinin B2 receptor peptide antagonist), des-Arg9-[Leu8]-bradykinin (DALBk; kinin B1 receptor peptide antagonist) were purchased from Sigma-Aldrich Chemical Company (St. Louis MO, USA) and were also prepared in NaCl (0.9%). The enzyme immunoassay kit for bradykinin was obtained from Phoenix Pharmaceuticals, Inc. (California, USA). Specific anti-B1 (bs-8675R–lot 9C20V14) or anti-B2 (bs-2422R–lot AG08307921) antibodies were acquired from Bioss Antibodies (Massachusetts, USA) and secondary antibody (sc-2357–lot L1218) was obtained from Santa Cruz Biotechnology (California, USA). Paclitaxel from semisynthetic ≥ 97% (in vitro assays) was acquired from Sigma-Aldrich Chemical Company (St. Louis MO, USA). Annexin-V and 3- (4, 5-dimethyl-2-thiazolyl) -2, 5-diphenyl-2H-tetrazolium bromide] salt (MTT; 5 mg/mL solution) were obtained from Life Technologies (São Paulo, BR). The 7-Amino-Actinomycin D (7AAD) was acquired from BD Biosciences (California, USA). The doses of the drugs used in this study were based on previous studies13 (link),15 (link),23 (link),40 (link),72 (link).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!