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Flow cytometric analysis program array software

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The FCAP Array software is a tool for analyzing data from flow cytometry experiments. It allows users to perform multi-analyte profiling by processing and analyzing data generated from bead-based multiplex assays.

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Lab products found in correlation

Cytometric bead array mouse inflammation kit, by BD (2 mentions) Lsrfortessa x 20 flow cytometer, by BD (2 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Facsdiva software, by BD (2 mentions) Phorbol 12 myristate 13 acetate pma, by Merck Group (2 mentions) Hepes ph 7, by Thermo Fisher Scientific (2 mentions) 2 mercaptoethanol, by Thermo Fisher Scientific (2 mentions) Ionomycin, by Merck Group (2 mentions) Il 33, by R&D Systems (2 mentions) Interleukin 7 (il 7), by R&D Systems (2 mentions) Cytometric bead array flex set, by BD (2 mentions) Il 18, by R&D Systems (2 mentions) Facscanto 2, by BD (1 mentions) Cba human soluble protein flex set capture bead kit, by BD (1 mentions) Serum separator tube, by BD (1 mentions) Cytometric bead array kit, by BD (1 mentions) Facsuite software, by BD (1 mentions) Fluorochrome conjugated monoclonal antibodies mabs, by BioLegend (1 mentions) Mouse il 22 elisa kit, by BioLegend (1 mentions) Cytokine bead array flex sets, by BD (1 mentions)

Spelling variants of this product

Flow cytometric analysis (33 citations) Flow Cytometric Analysis Program Array (4 citations)

7 protocols using flow cytometric analysis program array software

1

Multiplex Cytokine Profiling in Mice

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Six different inflammatory cytokine (IL-6, IL-10, MCP-1, TNF, IFN-γ, and IL-12p70) levels in mouse serum were determined using a Cytometric Bead Array Mouse Inflammation Kit (Becton Dickinson, Sparks, MD, USA) in accordance with the manufacturer’s protocol. Each specific antibody conjugated to capture beads and its target analyte combined to form sandwich complexes with PE-conjugated detection antibodies. Fluorescence signals were measured using flow cytometry (FACS Canto II; Becton Dickinson, Sparks, MD, USA), and the data were analyzed with Flow Cytometric Analysis Program Array software (version 3.0, Becton Dickinson, Sparks, MD, USA).
Park H., Yeo S., Kang S, & Huh C.S. (2021). Longitudinal Microbiome Analysis in a Dextran Sulfate Sodium-Induced Colitis Mouse Model. Microorganisms, 9(2), 370.
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2

Cytokine and Chemokine Profiling in PBMC and Plasma

Cited 1 time
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IFN-γ, TNF-α, granzyme B, IP-10, MIG, IL-2 and IL-10 were determined in 50 μl of supernatant from the PBMC cultures, and in the same volume of SLA-stimulated plasma from the WBA [25 (link)], using the CBA Human Soluble Protein Flex Set Capture Bead Kit (Becton Dickinson, USA), following the manufacturer's instructions. Results were captured by flow cytometry using Flow Cytometric Analysis Program Array software (Becton Dickinson, USA). Results from each cytokine and chemokine were expressed as the difference between the SLA-stimulated and control plasma concentrations.
Botana L., Ibarra-Meneses A.V., Sánchez C., Castro A., San Martin J.V., Molina L., Ruiz-Giardin J.M., Carrillo E, & Moreno J. (2019). Asymptomatic immune responders to Leishmania among HIV positive patients. PLoS Neglected Tropical Diseases, 13(6), e0007461.
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3

Culturing and Analyzing Sorted ILC2s

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Sorted ILC2s were cultured at 1500–3000 cells/well (37 °C, 5% CO2) for 24 h in 100 μl of media containing high glucose (4.5g/L) DMEM, 10% heat-inactivated FBS, 3.7g/L NaHCO3, 0.036g/L L-Asparagine, 0.116g/L L-Arginine, 0.006 g/L folic acid and supplemented with 10 mM HEPES pH 7.4, penicillin-streptomycin, and 2-mercaptoethanol (Life Technologies), plus IL-7, IL-18, IL-33, and and/or TSLP (all at 10 ng/ml; R&D Systems), or where indicated, ionomycin (500 ng/ml) and phorbol 12-myristate 13-acetate (PMA; 40 ng/ml; Sigma). Cell-free supernatants were collected after centrifugation for protein analysis, while cell pellets were resuspended and stained for flow cytometric analysis. Supernatants were assayed for IL-5 and IL-13 protein amounts using Cytometric Bead Array Flex Sets, acquired with a 5-laser LSRFortessa X-20 flow cytometer and BD FACSDiva software and analyzed using Flow Cytometric Analysis Program (FCAP) Array software (BD Biosciences).
Ricardo-Gonzalez R.R., Van Dyken S.J., Schneider C., Lee J., Nussbaum J.C., Liang H.E., Vaka D., Eckalbar W.L., Molofsky A.B., Erle D.J, & Locksley R.M. (2018). Tissue signals imprint ILC2 identity with anticipatory function. Nature immunology, 19(10), 1093-1099.
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4

Cytokine and IgE Quantification

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Supernatants from cell cultures (see above) were assayed using IL-5 and IL-13 Flex Sets with Cytometric Bead Array kit (BD). Bead fluorescence was captured on an LSRII (BD) and analyzed using Flow Cytometric Analysis Program (FCAP) Array software (BD). Blood was collected in serum separator tubes (BD) and centrifuged at 10,000 g for 10 minutes to isolate serum, which was assayed for IgE by ELISA MAX Standard IgE set (Biolegend). For lung cytokine measurements, the whole lung was homogenized, lysates were normalized to equal protein-containing volumes, and cytokine abundance was measured by ELISA as described23 (link).
Van Dyken S.J., Nussbaum J.C., Lee J., Molofsky A.B., Liang H.E., Pollack J.L., Gate R.E., Haliburton G.E., Ye C.J., Marson A., Erle D.J, & Locksley R.M. (2016). A tissue checkpoint regulates type 2 immunity. Nature immunology, 17(12), 1381-1387.
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5

Immunomodulatory Effects of ALT-803 in Mice

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B6 mice were injected iv or sc with ALT-803 or PBS as described above. Mice were humanely sacrificed and spleens were harvested at 0, 16, 24, 48, and 72 h following injection. For splenic immune cell phenotyping, splenocytes were isolated and stained with the fluorochrome-conjugated monoclonal antibodies (mAbs) against mouse CD4, CD8, NK1.1, NKp46, KLRG1, CD25, and intracellular Foxp3 and granzyme B molecules (Biolegend, San Diego, CA). The stained cells were analyzed on a FACSverse with FACSuite software (BD Biosciences, San Jose, CA). Serum cytokine levels of ALT-803-treated mice were also assessed using a Cytometric Bead Array, Mouse Inflammation Kit (BD Biosciences, San Jose, CA) according to manufacturer’s instructions. The data were analyzed using Flow Cytometric Analysis Program (FCAP) Array Software (BD Biosciences, San Jose, CA).
Liu B., Jones M., Kong L., Noel T., Jeng E.K., Shi S., England C.G., Alter S., Miller J.S., Cai W., Rhode P.R, & Wong H.C. (2018). Evaluation of the biological activities of the IL-15 superagonist complex, ALT-803, following intravenous versus subcutaneous administration in murine models. Cytokine, 107, 105-112.
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6

Culturing and Analyzing Sorted ILC2s

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Sorted ILC2s were cultured at 1500–3000 cells/well (37 °C, 5% CO2) for 24 h in 100 μl of media containing high glucose (4.5g/L) DMEM, 10% heat-inactivated FBS, 3.7g/L NaHCO3, 0.036g/L L-Asparagine, 0.116g/L L-Arginine, 0.006 g/L folic acid and supplemented with 10 mM HEPES pH 7.4, penicillin-streptomycin, and 2-mercaptoethanol (Life Technologies), plus IL-7, IL-18, IL-33, and and/or TSLP (all at 10 ng/ml; R&D Systems), or where indicated, ionomycin (500 ng/ml) and phorbol 12-myristate 13-acetate (PMA; 40 ng/ml; Sigma). Cell-free supernatants were collected after centrifugation for protein analysis, while cell pellets were resuspended and stained for flow cytometric analysis. Supernatants were assayed for IL-5 and IL-13 protein amounts using Cytometric Bead Array Flex Sets, acquired with a 5-laser LSRFortessa X-20 flow cytometer and BD FACSDiva software and analyzed using Flow Cytometric Analysis Program (FCAP) Array software (BD Biosciences).
Ricardo-Gonzalez R.R., Van Dyken S.J., Schneider C., Lee J., Nussbaum J.C., Liang H.E., Vaka D., Eckalbar W.L., Molofsky A.B., Erle D.J, & Locksley R.M. (2018). Tissue signals imprint ILC2 identity with anticipatory function. Nature immunology, 19(10), 1093-1099.
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7

Cytokine Profiling in Mouse Serum

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Cytokine levels in mouse serum were measured according to the manufacturer's protocol using the following Cytokine Bead Array Flex Sets (BD): IL-4 (#558298), IL-5 (#558302), IL-13 (#558349), and IL-17A (#560283). The data were analyzed using Flow Cytometric Analysis Program (FCAP) Array software (BD). Serum IL-22 was measured using LEGEND MAX TM (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
Mouse IL-22 ELISA Kit (BioLegend, #436307) and analyzed using a SpectraMax M2 microplate reader using SoftMax Pro software (Molecular Devices).
Ricardo-González R.R., Kotas M.E., Tenvooren I., Marquez D.M., Fassett M.S., Lee J., Daniel S.G., Bittinger K., Díaz R.E., Fraser J.S., Ansel K.M., Spitzer M.H., Liang H., & Locksley R.M. (2021). Type 2 innate immunity regulates hair follicle homeostasis to control Demodex pathosymbionts.
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