Insulin rodent mouse rat chemiluminescence elisa kit

Glucose-stimulated insulin secretion assay was performed as previously described.^{16 (link)} Briefly, control or SSR2/SSR4 null cells were incubated with prewarmed Krebs-Ringer bicarbonate Hepes [KRBH; 0.5% bovine serum albumin, 129 mM NaCl, 5 mM NaHCO3, 4.8 mM KCl, 1.2 mM KH2PO2, 2.5 mM CaCl2, 1.2 mM MgSO4, and 10 mM Hepes (pH 7.4)] at 37°C for 2 hours. The preincubation medium was removed, and cells were then incubated with KRBH containing 2.5 mM glucose for additional 2 hours. The culture media were collected, and the cells were then stimulated with KRBH containing 25 mM glucose for another 2 hours. After collecting the stimulation media, cells were lysed with acid-ethanol. Rat insulin levels in both media and cell lysates were assayed using the STELLUX Insulin Rodent (Mouse/Rat) Chemiluminescence ELISA kit (ALPCO No. 80-INSMR-CH01).