Multistix 10 sg reagent strip

Manufactured by Siemens

Sourced in United States

The Multistix 10 SG Reagent Strips are a laboratory diagnostic tool designed to test urine samples. The strips feature 10 reagent pads that can detect the presence and concentration of various analytes, including glucose, protein, and pH levels, among others. The strips provide a quick and convenient way to perform basic urinalysis tests in a clinical setting.

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16 protocols using multistix 10 sg reagent strip

Urine and Saline Dipstick Analysis

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All urine samples were evaluated using Multistix® 10 SG reagent strip (Siemens), and the results were recorded using an instrumental evaluation (Siemens). Saline collected after every catheterization was tested for presence of blood using the same type of dipstick as for the urine samples.

Stærk K., Schrøder B., Jensen L.K., Petersen T., Andersen T.E, & Nielsen L.F. (2023). Catheter‐associated bladder mucosal trauma during intermittent voiding: An experimental study in pigs. BJUI Compass, 5(2), 217-223.

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Urine Fluorescence Profiling in UTI

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Midstream urine from eight anonymous healthy volunteers was collected and immediately screened with a urine dipstick (Multistix® 10 SG reagent strip, Siemens, Germany). Urine cultures were performed by streaking 10 µl urine on Brilliance™ UTI Agar (Oxoid, UK), and incubating the plates at 37 °C. Next day, colony-forming units (CFUs) were counted, and bacterial species were identified based on the colony color. To investigate urine’s native fluorescence, 2 × 100 µl from each healthy urine sample were transferred in a transparent, flat-bottom 96-well plate, and excitation spectra of 300–520 nm with emission collected at 545 nm were collected using an Infinite® M1000 PRO plate reader (TECAN, Mannedorf, Switzerland).
Aliquots of 3–5 ml of urine samples were collected anonymously from 182 patients with confirmed UTI caused by >10⁵ CFU/ml of UPEC from Karolinska University Hospital, Stockholm, Sweden. All samples were immediately analyzed, and discarded after use.

Antypas H., Choong F.X., Libberton B., Brauner A, & Richter-Dahlfors A. (2018). Rapid diagnostic assay for detection of cellulose in urine as biomarker for biofilm-related urinary tract infections. NPJ Biofilms and Microbiomes, 4, 26.

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Evaluating Leukocyte Esterase Test for Sputum Grading

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We used the Multistix 10 SG reagent strip (Siemens Healthcare Diagnostics Inc., Munich, Germany) to evaluate the usefulness of the LE test in comparison with sputum grading by Gram stain. [17 (link),18 (link)]. The strip was dipped into the sputum specimens and read after 2 min according to the manufacturer’s instruction. Based on the color change, the results were recorded as negative, 1+, 2+, and 3+. The investigator who interpreted the culture results was blinded to the LE test results.

Yoo I.Y., Seok H.S., Kwon J.A., Lee J., Jo S., Kim S.Y, & Park Y.J. (2023). Evaluation of the BioFire® FilmArray® Pneumonia Panel with Conventional Bacterial Culture in Conjunction with Leukocyte Esterase Test. Diagnostics, 13(11), 1847.

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Comprehensive Clinical Laboratory Screening

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A comprehensive clinical laboratory screening confirmed the health status of the volunteers one month prior to challenge and at the time of diagnosis, and again at three weeks and four months after treatment was completed. Screening assays consisted of the following: automated whole blood cell counts, urine analysis by dipstick (Multistix 10 SG Reagent Strips, Siemens), blood chemistry tests for creatinine, blood urea nitrogen (BUN), glycemia, total bilirubin, direct bilirubin, transaminases (ALT and AST), coagulation tests, and C-reactive protein.

Arévalo-Herrera M., Forero-Peña D.A., Rubiano K., Gómez-Hincapie J., Martínez N.L., Lopez-Perez M., Castellanos A., Céspedes N., Palacios R., Oñate J.M, & Herrera S. (2014). Plasmodium vivax Sporozoite Challenge in Malaria-Naïve and Semi-Immune Colombian Volunteers. PLoS ONE, 9(6), e99754.

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Feline Urinary and Hematological Evaluation

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All cats received veterinary inspections at baseline and 30, 60, and 90 days. Urine and hematological analyses were performed at baseline (T0) and at the end of the evaluation (T1). Dipstick urinalysis was performed using Multistix^® 10 SG^® reagent strips (Siemens S.p.A, Milan, Italy). The reagent strip contained test pads for pH, urine specific gravity, and proteins. Urine color was graded according to the following score: dark = 0, clear = 1, and very clear = 2. Turbidity was graded according to the following score: limpid = 0, partially turbid = 1, and turbid = 2 (Canello et al. 2017). Hematological analyses [creatinine, blood urea nitrogen (BUN), phosphorus, potassium, calcium, albumin, hematocrit, alanine aminotransferase (ALT), and aspartate aminotransferase (AST)] were performed using Dimension RxL Max Integrated Chemistry System (Siemens Healthcare Diagnostics S.r.l., Milan, Italy).

Di Cerbo A., Iannitti T., Guidetti G., Centenaro S., Canello S, & Cocco R. (2018). A nutraceutical diet based on Lespedeza spp., Vaccinium macrocarpon and Taraxacum officinale improves spontaneous feline chronic kidney disease. Physiological Reports, 6(12), e13737.

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Kidney Function Assessment Protocol

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Kidney function was determined at different points by measuring serum BUN and serum creatinine using the Abaxis vet scan VS2 machine (Abaxis Inc., Union City, CA)³⁴. Urine protein was measured by using the Multistix 10 SG reagent strips (Siemens Healthcare Diagnostics Inc., Tarrytown, NY).

Kilari S., Yang B., Sharma A., McCall D.L, & Misra S. (2018). Increased transforming growth factor beta (TGF-β) and pSMAD3 signaling in a Murine Model for Contrast Induced Kidney Injury. Scientific Reports, 8, 6630.

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Comprehensive Pulmonary TB Diagnosis

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Eligible patients submitted two spot sputum samples (A&B) and up to 120mL of spontaneously voided urine. Though sputum induction was available, no patients required induction and all samples were expectorated. Sputum was concentrated for fluorescence smear microscopy (x2) and solid and liquid culture (x2). Urine was analyzed using Multistix 10SG Reagent Strips and Clinitek Analyzer (Siemens Healthcare Diagnostics, Tarrytown, NY, USA), and processed using the prototype concentration method and lateral flow assay (LFA). Patients that self-identified as HIV-positive had CD4+ T-cell counts measured using a BD FACSCalibur Flow Cytometer (BD Biosciences, San Jose, CA, USA). Patients who were unsure or reported negative were tested using the Alere Determine™ HIV Ag/Ab test and confirmed using other diagnostic tests in the algorithm. Patients with discrepant urine TB-LAM LFA and sputum Xpert results, specifically false positives, were followed-up at 1 and 2 months for repeat symptom assessment and sputum-based TB testing.

Connelly J.T., Andama A., Grant B.D., Ball A., Mwebe S., Asege L., Nakaye M., Lopez B.B., Hsieh H.V., Katumba D., Mukwatamundu J., Nalubega M., Hunt V.M., Burkot S., Ramachandraiah H., Choudhary A., Ignatowicz L., Weigl B.H., Bachman C., Mulondo J., Semitala F., Worodria W., Pinter A., Hamasur B., Bell D., Cattamanchi A, & Somoskovi A. (2021). Field evaluation of a prototype tuberculosis lipoarabinomannan lateral flow assay on HIV-positive and HIV-negative patients. PLoS ONE, 16(7), e0254156.

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Comprehensive Metabolic and Hematological Panel

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Urine, serum, and whole blood parameters were assessed in all participants at the baseline and final visits. Biochemical parameters were measured using Cobas C 311 (Roche Diagnostics, Rotkreuz, Switzerland), and hematological parameters were measured using the auto hematology analyzer Mindray BC-20 (Shenzhen Mindray Bio-Medical Electronics Co., Ltd, China). Urine analysis was carried out using standard Siemens Multistix 10 SG reagent strips and by microscopy of sediment.

Salter D., Swamy S., Salis K.M., Deep D.K, & Nadig P. (2024). A botanical extract blend of Mangifera indica and Sphaeranthus indicus combined with resistance exercise training improves muscle strength and endurance over exercise alone in young men: a randomized, blinded, placebo-controlled trial. Frontiers in Nutrition, 11, 1393917.

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Urine Collection and Analysis Protocol

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All participants provided rst-morning urine (20 ml -50 ml) in a sterile urine containers. Urine samples were obtained in the morning before scheduled renal biopsy (IgAN, FSGS patients) or surgery (renal cancer and prostate cancer patients). The specimens were processed at room temperature within 1-3 hours post-collection. All samples were tested for the following parameters: leukocyte esterase, nitrite, urobilinogen, protein, pH, blood, speci c gravity, ketone, bilirubin, glucose with a Multistix 10 SG Reagent Strips (Siemens Healthcare Diagnostics Inc, NY, USA). Urine specimens of healthy controls and prostate cancer patients had normal urine parameters. The summary of urine dipstick test results is included in Supplementary table 1. Urine samples were centrifuged at 4300g for 30 minutes at room temperature and the supernatants were aliquoted into 2 ml sterile LoBind tubes, and stored at -80 o C until further processing.

Marek-Bukowiec K., Konieczny A., Ratajczyk K., Macur K., Czaplewska P., Czyżewska A., Kowal P., & Witkiewicz W. (2020). The Value of Urinary RBP4 in The Diagnosis of FSGS and other Renal Diseases.

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Adherence and Satisfaction in Hydration Intervention

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Participant adherence was evaluated based on attendance at in-person sessions that included spot urines to measure hydration status, completion of telephone counseling calls, and self-reported dietary and water intake. We also evaluated participant satisfaction with the intervention.
Diet and physical activity were assessed by 3 unannounced 24-hour recall interviews (2 weekdays, 1 weekend day) at baseline and again at 6-months. The interviewer was masked to group assignment. Dietary data were collected using the Nutrition Data System for Research (NDSR) software versions 2010–2013, developed by the Nutrition Coordinating Center, University of Minnesota, Minneapolis. Final calculations were completed using NDSR version 2013. Physical activity data were collected using established methods ^{31 (link),32 (link)} and are presented as metabolic equivalents (METs) ^{33 (link)}. At each in-person session, participants provided a spot urine sample that was immediately analyzed for specific gravity (MultiStix 10 SG reagent strips; Siemens Healthcare Diagnostics Inc.). At the end of the study, participants responded to satisfaction questions related to the standard weight loss diet using 10-cm visual analog scales with appropriate verbal anchors. The Water group completed additional questions related to the “8×8” recommendation.

Wong J.M., Ebbeling C.B., Robinson L., Feldman H.A, & Ludwig D.S. (2017). Effects of advice to drink 8 cups of water per day in adolescents with overweight or obesity: a randomized trial. JAMA pediatrics, 171(5), e170012.

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