The running training was conducted using an electric treadmill (EP 131, Insight, Brazil) 2 weeks after MI induction, as previously described (de Almeida et al., 2014 (link)). The training period was divided into two phases. The first was a 1-week adaptation phase (10 min/day at 0.3 km/h) with progressive increases in time until the 5th day when the maximum time of 60 min was reached. From the 2nd week on, the duration of exercise was constant (60 min/day), but the exercise intensity was gradually increased from 0.3 km/h until reaching a speed of 1.2 km/h. ET was performed five times per week for a total of 8 weeks.
Ep 131
Manufactured by Dotmatics
Sourced in Brazil
The EP 131 is a compact and portable electroporator designed for efficient DNA/RNA transfer into a variety of cell types. It features an intuitive user interface and adjustable voltage and pulse duration settings to optimize electroporation conditions for different samples.
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Lab products found in correlation
14 protocols using ep 131
1
Post-Infarct Exercise Training Protocol
2
Treadmill Exercise Training Post-Myocardial Infarction
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Exercise training was performed on a motorized treadmill (EP 131, Insight, Brasil). The training protocol consisted of a modification of a protocol used previously for training MI rats. [5] (link) Two weeks after infarction, the animals subjected to exercise training were adapted to a treadmill for one week (10 min/d; 0.3 Km/h); the regimen increased daily by ten minutes until reach sixty minutes on the fifth day. From the second week on, exercise duration was constant (60 min/day). The intensity was gradually increased in speed from 0.3 to 1.2 km/h, and performed 5 times per week, with two days of rest during the 8 week period. Animals rested for 48 h (to analyze the effects of chronic exercise) before undergoing hemodynamic evaluation.
3
Graded Treadmill Running to Fatigue
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Four weeks after the surgery, rats performed a graded treadmill run to fatigue on a customized rodent treadmill (EP–131, Insight, São Paulo, Brazil). The protocol involved the rats running in three steps, with a progressive increase in treadmill speed: 1) 8 m min-1, 2) 12 m min-1, and 3) 18 m min-1. The two initial steps lasted for 3 min each, whereas rats continued running in the final step until they reached the point of fatigue, which was confirmed by the loss of the animal righting reflex. Subsequently, the total running distance was obtained.
4
Graded Treadmill Test in Rats
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Rats performed a graded treadmill test on a customized rodent treadmill (EP–131, Insight, São Paulo, Brazil). In the fourth week, a treadmill test was conducted as follows: after acclimatization for 3 minutes in the cages, the animals were induced to run for 3 minutes at 8 m.min−1, 3 minutes at 12 m.min−1, and until volitional fatigue at 18 m.min−1. Exercise intolerance was determined by measuring the running distance until volitional fatigue, which was confirmed by loss of the animal’s righting reflex.20 (link)
5
Moderate-Intensity Aerobic Exercise in Hamsters
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After 12 weeks of HF feeding, hamsters (5 to 6 months of age) were randomly assigned to an exercise practice (n = 60). One week before the beginning of the exercise protocol, they were placed in the treadmill machine at lower speed for a short time for adaptation. The exercise training consisted of 8 weeks of running on a motor treadmill (INSIGHT – EP 131 – Ribeirão Preto, SP, Brazil), 5 times/week, raising weekly time and speed until the limit of 60 min at 18 m/min (Figure 1 ) [19] (link). Exercise was conducted at 50–70% of VO2 maximum, featuring the overall AET protocol as of moderate intensity. Measures of RQ (respiratory quotient) were set at 1 to designate respiratory exhaustion and conducted in 0.89, characterizing the exercise as aerobic. Animals did not pass through exercise training 24–36 h before the experimental day.
6
Estrogen Impacts Exercise Tolerance in SHR
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Given that exercise intolerance is often the first clinical manifestation of diastolic dysfunction, a maximal exercise tolerance test (time to exhaustion during a standardized exercise protocol) was performed to differentiate functional disparities between SHR and WKY strains based on estrogen status. A total of four rats in each group were familiarized with the customized rodent treadmill (EP-131, Insight, São Paulo, Brazil) by walking at a speed of 20 cm/s, 10 min/d, and 5% grade for 1 week prior to testing. Each exercise test was performed after at least 1 day of rest. The protocol for the exercise tolerance evaluation consisted of 3 min at 12 m/min, with 1.2 m/min increases in speed every 3 min until the rats reached exhaustion. Exhaustion was determined when the rats stopped keeping pace with the treadmill and remained at the lower end of the treadmill for more than 5 s, despite gentle nudging by an investigator (DGC) who was blinded to the study groups. The total distance travelled (meters) was used as a measure of exercise capacity.
7
Low-Intensity Treadmill Exercise in mdx Mice
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The exercise protocol started at the day after the first assessment (zero) of body weight. The animals of mdxE group underwent a LIT protocol on a horizontal treadmill (EP 131; Insight, Ribeirão Preto, Brazil) 30 min/day, 3 times a week for 60 days at a velocity of 9 m/min. The protocol speed was controlled at 9 m/min so that the therapeutic training was considered of low intensity (Grange, 2014 ). Before the experimental period, the animals underwent a period of adaptation in order to familiarize with the apparatus and speed, which was gradually increased. Aiming to offer the same environmental stimuli to experimental mice, the animals of mdxC group were placed at the off treadmill (0 m/min) with the same duration and frequency of the animals of mdxE group.
Body weight (g) of all animals was assessed over the time of the exercise protocol (0, 15, 30, 45, and 60 days). On the 60th day of protocol, samples of tibialis anterior (TA) muscle were harvested for analysis. Morphological, histochemical and immunohistochemistry analysis were all conducted in this same muscle group.
Body weight (g) of all animals was assessed over the time of the exercise protocol (0, 15, 30, 45, and 60 days). On the 60th day of protocol, samples of tibialis anterior (TA) muscle were harvested for analysis. Morphological, histochemical and immunohistochemistry analysis were all conducted in this same muscle group.
8
Exercise Performance in Pulmonary Hypertension
9
Estrogen Impacts Exercise Tolerance in SHR
10
Treadmill Exercise Regimen in Rodents
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Animals were habituated on a motor-driven treadmill (Insight EP 131, Brazil) and put to run for 5 days to 10, 15, 20, 25, and 30 min/day and the incremental speeds/day was 10, 12, 13, 14, 15 m/min (0° slope). After that, the rats were randomly divided into four groups: sedentary-saline, sedentary-LPS, exercise-saline and exercise-LPS. Exercise groups ran for 4 weeks. Briefly, in the 1st week, the animals ran at 15 m/min for 30 min/day. Then, treadmill speed and exercise duration were progressively increased to 17 m/min for 40 min/day in the 2nd week. During the 3rd week the speed was 19 m/min for 50 min/day and on the 4th week the rats ran at 20 m/min for 60 min/day. This method was adopted from previous studies in rat [12 ] and in mice [13 (link)].
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