Culture media from hippocampal neurons infected with Sindbis virus expressing human APP were collected after PTX stimulation and BI2536 treatment. N2a cell media were collected 24h after vector or Plk2 transfection. Collected media were analyzed for human Aβ40 and Aβ42 using species-specific sandwich ELISA (Invitrogen) as described (Lee et al., 2010 (link)).
Species specific sandwich elisa
Manufactured by Thermo Fisher Scientific
The Species-specific sandwich ELISA is a laboratory equipment used for the detection and quantification of specific proteins or analytes in a sample. It is a type of enzyme-linked immunosorbent assay (ELISA) that utilizes antibodies specific to the target analyte to capture and detect it in the sample.
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Lab products found in correlation
2 protocols using species specific sandwich elisa
1
Quantifying Amyloid-beta Production
2
Extraction and Quantification of Amyloid-β in Mouse Forebrain
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Mouse forebrains were homogenized in cold 5 M guanidine HCl and 50 mM Tris pH 8.0 with protease inhibitors and mixed for 3–4 hours. High concentration of guanidine HCl was used a chaotropic agent to extract insoluble Aβ and final concentration was below 0.1M [37 (link)–39 (link)]. The sample was diluted with cold reaction buffer (PBS with 5% BSA and 0.03% Tween-20) with protease inhibitors and centrifuged at 16,000 x g for 20 min, and the supernatant saved for assay. Extracts were analyzed for mouse Aβ40 as well as human Aβ40 and Aβ42 using species-specific sandwich ELISA (Invitrogen) as described [40 (link)].
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