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Bs 3602r

Manufactured by Bioss Antibodies

The Bs-3602R is a laboratory centrifuge designed for high-speed separation of samples. It features a maximum speed of 6,000 rpm and a maximum relative centrifugal force (RCF) of 3,602 x g. The centrifuge can accommodate a variety of rotor types and sample volumes, making it suitable for a wide range of applications in research and diagnostic laboratories.

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3 protocols using bs 3602r

1

Western Blot Analysis of Muscle Proteins

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Western blot analysis was performed as previously described.66 (link) The primary antibodies used were anti-FLAG (AF519, Beyotime, 1:1,000), anti-MYOD (ABP53067, Abbkine, 1:500), anti-MyHC (B103, DHSB, 0.5 μg/mL), anti-SERCA2 (NB100-237, Novus, 1:2,000), anti-ACC (PA5-17564, Thermo Fisher Scientific, 1:1,000), anti-pACCSer80 (orb315750, Biorbyt, 1:500), anti-CPT1 (bs-23779R, Bioss, 1:500), anti-pmTORSer2488 (#5536, CST, 1:1,000), anti-mTOR (bs-1992R, Bioss, 1:500), anti-ULK1 (bs-3602R, Bioss, 1:500), anti-LC3B (NB100-2220, Novus, 2.0 μg/mL), anti-P62 (18420-1-AP, Proteintech, 1:1,000), anti-CREB (bs-0035R, Bioss, 1:500), anti-pCREBSer133 (ab32096, Abcam, 1:5,000), anti-AMPK (bs-1115R, Bioss, 1:500), anti-pAMPKser712 (ABN-PAB12602, Abnova, 1:2,000), anti-Calcineurin (#2614S, CST, 1:1,000), anti-PGC-1α (66369-1-Ig, Proteintech, 1:5,000), and anti-GAPDH (60004-1-Ig, Proteintech, 1:5,000). ProteinFind goat anti-mouse IgG(H+L), HRP conjugate (HS201-01, TransGen, 1:1,000) and ProteinFind goat anti-rabbit IgG(H+L), HRP conjugate (HS101-01, TransGen, 1:500) were used as secondary antibodies.
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2

Western Blot and Immunofluorescence Analysis

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Western blots were performed as previously described [50 (link)]. The primary antibodies used were anti-FLAG (AF519, 1:1,000, Beyotime), anti-ACACA (PA5-17564, 1:1000, Thermo Fisher Scientific), anti-p-ACACA Ser80 (orb315750, 1:500, Biorbyt), anti-PC (GTX132002, 1:500, GeneTex), anti-MYOD (ABP53067, 1:500, Abbkine), anti-MyHC (B103, 0.5 μg/ml, DHSB), anti-CPT1 (bs-23779R, 1:500, Bioss), anti-FASN (10624-2-AP, 1:200, Proteintech), anti-p-mTOR Ser2488 (#5536, 1:1000, CST), anti-mTOR (bs-1992R, 1:500; Bioss), Ubiquitin (#39361:1000, CST) anti-ULK1 (bs-3602R, 1:500; Bioss), anti-LC3B (NB100-2220, 2.0 ug/ml, Novus), anti-P62 (18420-1-AP, 1:1000, Proteintech) and anti-β-Tubulin (A01030, 1:10000, Abbkine). ProteinFind Goat Anti-Mouse IgG(H + L), HRP Conjugate (HS201-01, 1:1,000, TransGen) and ProteinFind Goat Anti-Rabbit IgG(H + L), HRP Conjugate (HS101-01, 1:500, TransGen) were used as a secondary antibody.
Immunofluorescence was performed using anti-MyHC (B103, 2.5 μg/ml, DHSB), as previously described [50 (link)]. A fluorescence microscope ((DMi8; Leica, German) was used to capture three randomly selected fields to visualize the area labeled with anti-MyHC.
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3

Western Blot Analysis of Muscle Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Western blot analysis was performed as previously described [44 (link)]. The primary antibodies used were anti-FLAG (AF519, Beyotime,1:1,000), anti-MYOD (ABP53067, Abbkine, 1:500), anti-MyHC (B103, DHSB, 0.5 mg/mL), anti-FASN (10624-2-AP, Proteintech, 1:200), anti-CPT1 (bs-23779R, Bioss, 1:500), anti-ULK1 (bs-3602R, Bioss,1:500), anti-LC3B (NB100-2220, Novus, 2.0 mg/mL), anti-P62 (18420-1-AP, Proteintech, 1:1,000), anti-TBP (44059, Cell signaling, 1:1000), anti-KLF4 (bs-1064R, Bioss,1:500), anti-GPI (GTX113203, GeneTex, 1:500), anti-TNNI2 (bs-10617R, Bioss, 1:500), anti-CDKN1A (GTX112898, GeneTex, 1:500), and anti-GAPDH (60004-1-Ig, Proteintech, 1:5,000). ProteinFind goat anti-mouse IgG(H + L), HRP conjugate (HS201-01, TransGen, 1:1,000) and ProteinFind goat anti-rabbit IgG(H + L), HRP conjugate (HS101-01, TransGen, 1:500) were used as secondary antibodies. The original images of western blot are shown in Additional file 1.
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