Sam chloride dihydrochloride

Animal experiments were licensed under the Animals (Scientific Procedures) Act of 1986 (UK) and were approved by the animal welfare committees at the University of Manchester. Eight- to twelve-week-old C57BL/6J male mice were purchased from Charles River (UK) and acclimatized to the local animal unit for 1 week before the experiment. Mice were housed under a 12:12 h light/dark cycle at ~350 lux during the light phase and 0 lux during the dark phase. Ambient temperature was kept at 22 ± 3 °C, the humidity was ~52 ± 4%, with food and water available ad libitum. Meal diets containing 127 mg/kg or 318 mg/kg SAM chloride dihydrochloride (Sigma A7007) were ordered from Special Diets Services, using their standard maintenance diet #BK001 as a base. Control diet was the same meal diet, BK001. Running wheel activity rhythms were measured in single-mouse cages equipped with running wheels (Vet Tech Solutions Ltd) placed in light-tight programmable cabinets (Tecniplast). Actograms and period estimates (chi-square periodogram) were acquired with Clocklab (Actimetrics).