Glucono δ lactone gdl

Manufactured by Merck Group

Sourced in United States

Glucono-δ-lactone (GDL) is a naturally occurring food-grade chemical compound. It functions as a pH regulator, coagulant, and gelling agent in various applications.

Automatically generated - may contain errors

Lab products found in correlation

9 protocols using glucono δ lactone gdl

Alginate Hydrogel Formation Protocols

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Viscoelastic gels were formed following a modified internal calcium carbonate gelation protocol[28 (link)]. Alginate in PBS was mixed with (1) CaCO₃ at 50 mg/mL and (2) glucono-δ-lactone (GDL; Sigma Aldrich, St. Louis, MO) at 150–200 mg/mL with a volume ratio of 8:1:1. The variation in GDL content controlled the amount of calcium ions liberated for crosslinking via lowering the pH, thereby varying the stiffness of the resulting gel. The mixture was pipetted into molds of 8 mm diameter and 1.5 mm height and allowed to gel at 37°C for 3 h.
Elastic gels underwent carbodiimide covalent crosslinking as described previously[21 (link)]. Alginate in MES buffer was mixed with (1) 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC; Sigma Aldrich) at 100 mg/mL and (2) a solution of adipic acid dihydrazide (AAD) and 1-hydroxybenzotriazole (1-HOBT; Sigma Aldrich), both at 25–75 mM. The mixture had a volume ratio of 8:1:1 alginate:EDC:AAD/1-HOBT. We controlled the number of crosslinks and resultant stiffness of the gel by variation in AAD content. The mixture was pipetted into identical molds as for viscoelastic hydrogels and allowed to gel under the same conditions. In both cases, the final concentration of alginate within the gels was 20 mg/mL.

Hung B.P., Gonzalez-Fernandez T., Lin J.B., Campbell T., Lee Y.B., Panitch A., Alsberg E, & Leach J.K. (2020). Multi-peptide presentation and hydrogel mechanics jointly enhance therapeutic duo-potential of entrapped stromal cells. Biomaterials, 245, 119973.

+ Open protocol

+ Expand

Anhydrous Milk Fat and Saliva Collection

Check if the same lab product or an alternative is used in the 5 most similar protocols

Anhydrous milk fat (fat content 99.9%, Friesland Campina, The Netherlands), skimmed milk powder (Anchor, Auckland, New Zealand) and skim milk (Yili Ltd., Inner Mongolia, China) were used. Chemical-grade oleic acid glyceride (CP, 60%) was purchased from Shanghai Macklin Biochemical Company, China, while glucono-δ-lactone (GDL), chemical-grade glycerin stearate (99.8%) and sodium dodecyl were purchased from Sigma–Aldrich (St. Louis, MO, USA). Food-grade glyceride stearate and oleic acid glyceride were obtained from Shi Hua Shi Shuo Raw Material Shop (Hebei, China).
Salivette (SARSTEDT, Dachau, Germany) was used to collect human saliva [12 (link)]. Five males and five females (23–26 years old, healthy adults) were selected. After saliva collection, all of the tubes were centrifuged (1000× g, 5 min), and then the centrifuged saliva was mixed thoroughly and stored at −80 °C.

Zhou H., Zhao Y., Fan D., Shen Q., Liu C, & Luo J. (2022). Effect of Solid Fat Content in Fat Droplets on Creamy Mouthfeel of Acid Milk Gels. Foods, 11(19), 2932.

+ Open protocol

+ Expand

Skim Milk Preparation and Protein-free Serum Extraction

Check if the same lab product or an alternative is used in the 5 most similar protocols

Pasteurized skim milk was obtained from Berkey Creamery, Pennsylvania State University. Raw, whole milk received by the creamery was subjected to cream separation and pasteurization (73°C, 15 s) to produce skim milk with a TS content of ~8.8%. Protein-free serum (PFS), which was used for the dilution of skim milk samples was obtained by ultrafiltration of same skim milk using a spiral-wound cartridge with a 3-kDa molecular weight cut-off (Millipore, Billerica, MA). Glucono-δ-lactone (GDL), which was used to adjust the pH of skim milk samples was purchased from Sigma (St. Louis, MO).

Hettiarachchi C.A., Swulius M.T, & Harte F.M. (2020). Assessing constituent volumes and morphology of bovine casein micelles using cryo-electron tomography. Journal of dairy science, 103(5).

+ Open protocol

+ Expand

Fabrication and Characterization of Biomimetic Hydrogels

Check if the same lab product or an alternative is used in the 5 most similar protocols

Silicone (SI 595 CL) was purchased from Ellsworth Adhesives. Alginic acid sodium salt from brown algae, calcium carbonate, hexadimethrine bromide, bradykinin acetate salt (BK; MW = 1.1 kDa), flourescein isothiocyanate (FITC)-labeled dextran (MW = 10 kDa), and glucono-δ-lactone (GDL) were purchased from Sigma-Aldrich. Dulbecco’s modified Eagle’s medium/F12 (DMEM/F12), bovine serum albumin (BSA), penicillin-streptomycin (10 000 U mL⁻¹), recombinant human basic fibroblast growth factor (FGF; AA 1–15; MW = 17.2 kDa), recombinant human epidermal growth factor (EGF; MW = 6.2 kDa), and antibiotic-antimycotic (Anti-Anti, 100X) were purchased from Thermo-Fisher. Fetal bovine serum (FBS) was from Aleken Biologicals.

Haring A.P., Thompson E.G., Hernandez R.D., Laheri S., Harrigan M.E., Lear T., Sontheimer H, & Johnson B.N. (2019). 3D Printed Multiplexed Competitive Migration Assays with Spatially Programmable Release Sources. Advanced biosystems, 4(1), e1900225.

+ Open protocol

+ Expand

Gelation of Sodium Caseinate by GDL

Check if the same lab product or an alternative is used in the 5 most similar protocols

Gels were synthesized by acidification of the NaCas solutions (0.026 concentration) with glucono-δ-lactone (GDL) (Sigma Chemical, USA) in a ratio GDL/NaCas of 0.3.
After mixing for 2 min, the solution was distributed in three glass Petri dishes (42 mm internal diameter, 7 g of NaCas solution per dish), covered with the corresponding glass cap and heated in an oven at 40 °C for 2 h. Under these conditions, the gel point was estimated from our own rheometric and pH measurements to occur after about 45.2 min at pH 4.87 (results not shown). The resulting gels were stored in a fridge at 5 ± 2 °C during three days after which gels color and water holding capacity were determined.

Costo R., Martínez P.D.S., & Guignon B. (2021). Pressure‐induced modification of sodium caseinate techno‐functional properties. Journal of Food Process Engineering, 44(7).

+ Open protocol

+ Expand

Whey Protein Isolate and Skim Milk Powder Formulation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Whey protein isolate (WPI) containing 93.4% protein (wet basis), 0.2% lactose, and 1.9% minerals was donated by Davisco Foods Intl. (Le Sueur, MN). Skim milk powder (SMP) containing 35% protein was obtained from a bulk manufacturer. Polysaccharides consisted of LM12 and HM D (CP Kelco, Atlanta, GA), and guar gum (TIC Gums, Belcamp, MD). Acidulation was achieved with either glucono-δ-lactone (GDL) provided by Sigma-Aldrich Corp. (St. Louis, MO) or yogurt cultures containing Streptococcus thermophilus and Lactobacillus bulgaricus provided by DuPont Danisco (Madison, WI). All other chemicals were of analytical grade.

O'Chiu E, & Vardhanabhuti B. (2017). Utilizing whey protein isolate and polysaccharide complexes to stabilize aerated dairy gels. Journal of dairy science, 100(5).

+ Open protocol

+ Expand

Uniform pH Reduction via GDL

Check if the same lab product or an alternative is used in the 5 most similar protocols

Glucono-δ-lactone (GDL, ref G2164, purity ≥99%, Sigma-Aldrich) was used to reduce the pH of the suspension uniformly and gradually. For φ v < 0.1, a concentration of 1 %wt GDL set as a standard condition was used to reach a final pH close to 4 in less than 1 h. For φ v > 0.1, the amount of GDL was increased to reproduce the standard acidification kinetics [6] (link). GDL was dispersed in the suspension by stirring for 1 min, before loading into the Couette cell of the rheometer.

de Oliveira Reis G., Gibaud T., Saint-Michel B., Manneville S., Leocmach M., Vaysse L., Bonfils F., Sanchez C, & Menut P. (2019). Irreversible hardening of a colloidal gel under shear: The smart response of natural rubber latex gels. Journal of colloid and interface science, 539.

+ Open protocol

+ Expand

Crosslinking Approach for Porous Materials

Check if the same lab product or an alternative is used in the 5 most similar protocols

The buffer was changed to 0.02 M 2-(N-morpholino)ethanesulfonic acid sodium salt (MES) (Sigma Aldrich) and 0.15 M NaCl before crosslinking. Crosslinking agents were N-(3dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC) and Nhydroxysulfosuccinimide sodium salt (sulfo-NHS) (Sigma Aldrich) in 0.02 M MES and 0.15 M NaCl for 16 hours. EDC was either set to 100 mM or 200 mM, and the concentration of sulfo-NHS was kept constant at 50 mM. Glucono-δ-lactone (GDL) (Sigma Aldrich) was used to dissolve the template core at pH 6.5 in a ratio GDL:CaCO3 2:1. For removal of impurities after crosslinking, dialysis with a membrane of 8-10 kDa in pore size was used (Spectrum Laboratories Inc., USA).

Craig M ., Altskär A ., Nordstierna L , & Holmberg K . (2016). Bacteria-triggered degradation of nanofilm shells for release of antimicrobial agents. Journal of materials chemistry. B, 4(4).

+ Open protocol

+ Expand

Milk Gel Imaging Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Milk fat was stained for imaging with Nile Red (Sigma Aldrich, UK) to a concentration of 2.5 μM in the milk. Stained samples were left overnight at 5°C. Milk acid gels were prepared by adding 0.25 g of Glucono-δ-Lactone (GDL)(Sigma-Aldrich, USA) to 10 mL of the pre-stained milk giving a concentration of 2.5% GDL in the milk. The 10 mL of milk with GDL was inverted by hand for 1 min, and 600 μL of milk was sampled to which 3 μL of Atto 488 NHS-Ester (Atto-Tec GmbH, Siegen, Germany), dissolved in DMSO (99.9% pure, Sigma-Aldrich) was added to give a concentration of 510 μM in the milk. This volume was then transferred to a μ-Slide 8 Well chamber (ibidi, Germany), and incubated at 35 °C for 90 min. Five gels were produced per milk sample, giving 10 gels per treatment, as milk samples were prepared in duplicate.

Glover Z.J., Gregersen S.B., Wiking L., Hammershøj M., & Simonsen A.C. (2021). Microstructural changes in acid milk gels due to temperature‐controlled high‐intensity ultrasound treatment: Quantification by analysis of super‐resolution microscopy images. International Journal of Dairy Technology, 75(2), 321-328.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!