Murashige and Skoog (MS) medium, ethanol, sucrose, ammonium nitrate, calcium nitrate tetrahydrate, ethylenediaminetetraacetic acid ferric sodium salt, potassium nitrate, potassium hydroxide, magnesium sulfate heptahydrate, potassium phosphate monobasic, magnesium chloride hexahydrate, boric acid, manganese (II) sulfate monohydrate, zinc sulfate heptahydrate, copper (II) sulfate pentahydrate, molybdenum (VI) oxide, sodium selenate, L-selenocystine, sodium borohydride, sodium selenite, trolox and Folin & Ciocalteu’s phenol reagent were obtained from the Merck – Sigma group (St. Louis, MO, USA). Phytoagar was purchased from Research Products International (Mt. Prospect, IL, USA). Pyridine (a. r.) was obtained from Carlo Erba (Peypin, France), while formic acid was purchased from Scharlau (Barcelona, Spain). Standards for ICP-OES calibration were obtained from Elemental Scientific (Omaha, NE, USA).
Magnesium sulfate heptahydrate
Manufactured by Merck Group
Sourced in United States, Germany, Belgium, United Kingdom
Magnesium sulfate heptahydrate is a chemical compound with the formula MgSO4·7H2O. It is a crystalline solid that is soluble in water and is commonly used in laboratory settings.
Automatically generated - may contain errors
Lab products found in correlation
Sodium chloride (nacl), by Merck Group (14 mentions)
Calcium chloride dihydrate, by Merck Group (8 mentions)
Potassium chloride (kcl), by Merck Group (8 mentions)
Sucrose, by Merck Group (5 mentions)
Hydrochloric acid (hcl), by Merck Group (5 mentions)
Glucose, by Merck Group (4 mentions)
Starch, by Merck Group (4 mentions)
Sodium bicarbonate, by Merck Group (4 mentions)
Potassium phosphate, by Merck Group (4 mentions)
Potassium hydroxide, by Merck Group (3 mentions)
Ammonium nitrate, by Merck Group (3 mentions)
Potassium phosphate monobasic, by Merck Group (3 mentions)
Zinc sulfate heptahydrate, by Merck Group (3 mentions)
Copper 2 sulfate pentahydrate, by Merck Group (3 mentions)
Ethanol, by Merck Group (3 mentions)
Magnesium chloride hexahydrate, by Merck Group (3 mentions)
Boric acid, by Merck Group (3 mentions)
Peptone, by Thermo Fisher Scientific (3 mentions)
Yeast extract, by Merck Group (3 mentions)
Calcium chloride dehydrate, by Merck Group (3 mentions)
39 protocols using magnesium sulfate heptahydrate
1
Comprehensive Plant Growth Media Protocol
2
Biofunctionalized Polymer Surfaces for Biosensing
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3,4-dihydroxyphenethylamine hydrochloride (Dopamine hydrochloride), 3,4-Dihydroxyl-L-phenylalaline (L-DOPA) (98% TLC), L-Tyrosine (98% HPLC), L-Ascorbic acid (99%), Phosphate-buffered saline (PBS) tablets, N,N-Dimethylformamide (DMF) (99.9% HPLC), 1-Pyrenebutynic acid N-hydroxy-succinimide ester (PBASE) (95%), 1-Dodecanethiol (DDT) (98%), Ethanolamine (ETA) (98%), Sodium Chloride (NaCl), Potassium Chloride (KCl), Monosodium Phosphate (NaH2PO4), Sodium Bicarbonate (NaHCO3), Glucose, Calcium Chloride Dihydrate (CaCl2.2H2O), Acetic Acid Glacial, Hydrochloric acid (HCl) (35%), Sodium Hydroxide (NaOH) tablets, and Poly(methyl(meth)acrylate) (PMMA) (15 k M.W.) were purchased from Sigma-Aldrich. Acetone (99.5%), Ethanol (99.8%), and 2-Propanol (99.8% GC) were purchased from Honeywell. Magnesium Sulfate Heptahydrate (MgSO4.7H2O) was purchased from Merck. Photoresist AZ1505 (AZ) was purchased from MicroChemicals GmbH. RTV silicone elastomer (3140, Dowsil) and superglue (Loctite) were acquired from Farnel. DNA aptamer (5′-CGACGCCAGTTTGAAGGTTCGTTCGCAGGTGTGGAGTGACGTCG-3′) with a 5′ C6-amino link modification was synthesized by Stab Vida. Reserpine was acquired from Biogen. MilliQ water used in all experiments had a resistivity higher than 18 MΩ cm at 25 °C.
3
Comprehensive Plant Growth Media Protocol
4
Compound Characterization and Stability of JME-173
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Sodium chloride, potassium chloride, potassium dihydrogen phosphate, sodium hydrogen carbonate, magnesium sulfate heptahydrate, and calcium chloride dehydrate were purchased from Merck (Darmstadt, Germany). Glucose, EGTA, carbachol, methacholine, theophylline, mexiletine, propranolol, SQ22,536, lipopolysaccharide (LPS), sodium pentobarbital, pancuronium bromide, sodium orthovanadate, and tetraethylammonium (TEA) were purchased from Sigma-Aldrich (St. Louis, MO). Isoflurane was obtained from Cristália (São Paulo, Brazil). JME-173 was synthesized and provided by the Laboratory of Organic Chemistry (Farmanguinhos, FIOCRUZ, RJ, Brazil). GCMS spectrum revealed that JME-173 is a 100% pure compound as also attested by the 1H NMR analysis and by its melting point 220–222°C. As predicted by the ChemAxon program (Version 19.22.0), the aqueous solubility at pH 6.4, 6.8, and 7.2 of JME-173 were 127.0, 50.6, and 20.2 mg/ml, respectively. There was no observed evidence of JME-173 degradation following long-lasting exposure to blood samples, storage at room temperature or −70°C, or after the repeated freeze and thaw cycles, pointing out the excellent physical stability of this compound (Pinto at al., under review in the Eur. J. Pharmacol.). All solutions were freshly prepared in distilled water and protected from light.
5
Cardiovascular Pharmacology Reagents Protocol
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Sodium pentobarbital (Dorminal 20%) was bought as an injectable solution (200 mg/ml, w/v) from Alfasan Woerden-Holland. Dimethyl sulfoxide (DMSO), sodium chloride (NaCl), potassium chloride (KCl), calcium chloride dihydrate (CaCl2.2H2O), magnesium sulfate heptahydrate (MgSO4.7H2O), potassium dihydrogen phosphate (KH2PO4), D (+)-glucose, and sodium hydrogen carbonate (NaHCO3) were purchased from Merck, Germany. Carbogen (a mixture of 95% O2 and 5% CO2) was purchased from Linde (M) Sdn. Bhd. Serotonin hydrochloride, phenylephrine hydrochloride, atropine sulfate, Nω-nitro-l-arginine methyl ester (L-NAME), propranolol hydrochloride, and phentolamine hydrochloride were purchased from Sigma®, USA.
6
Trastuzumab-Producing CHO-GS(-/-) Cell Line
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IgG1 protein (Trastuzumab) producing CHO-GS(-/-) cell line was used and was provided by an industrial collaborator (Imgenex®, Bhubaneswar, India). The cell line is suitable for growth in glutamine-free media, once transfected with the vector containing glutamine synthetase gene (GS). A commercially available proprietary cell culture medium CD CHO® (Gibco™—12,490–001) was used as a basal medium, and Efficient Feed B medium (Gibco™—A1245605) as a medium feed supplement for fed-batch culture. Metal salts, copper (II) chloride dihydrate, magnesium sulfate heptahydrate, manganese chloride, iron sulfate heptahydrate, cobalt sulfate heptahydrate, zinc chloride, and nickel (II) chloride hexahydrate were purchased from Merck (Kenilworth, NJ, USA).
7
Characterization of PEG-based Nanofiltration Membranes
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CC (99%),
sodium hydroxide (NaOH, puriss. p.a., ACS reagent ≥98%), sodium
chloride (NaCl, ≥99%), sodium dodecyl sulfate (SDS, ACS reagent
≥99.0%), and poly(ethylene glycol) (PEG) with molecular weights
of 400, 600, and 1500 g mol–1 were obtained from
Sigma Aldrich (Germany).
Sodium sulfate (Na2SO4, anhydrous, for analysis EMSURE ACS), magnesium sulfate heptahydrate
(MgSO4, for analysis EMSURE ACS), and PEG 1000 (EMPROVE
ESSENTIAL) were obtained from Merck Millipore (Germany).
Magnesium
chloride hexahydrate (MgCl2, 99.0–101.0%,
AnalaR NORMAPUR ACS Reag.) was purchased from VWR chemicals (Netherlands). n-Hexane (anhydrous, over molecular sieves) was acquired
from Alfa Aesar (Germany). 1,1,1-Tris(4-hydroxyphenyl)ethane (TPE,
>98.0%) was acquired from TCI (Belgium). PEG 200 was obtained from
Fluka (Germany).
Milli-Q water was used to prepare all aqueous
solutions. All chemicals
were used as received.
Hydrophilized polyethersulfone (PES)
ultrafiltration (UF) membranes
with a MWCO of 30 kDa were purchased from Microdyn-Nadir (UH030, Germany)
and used as the support. According to the supplier and earlier research,
these supports are stable in the pH range 0–14.11 (link)
sodium hydroxide (NaOH, puriss. p.a., ACS reagent ≥98%), sodium
chloride (NaCl, ≥99%), sodium dodecyl sulfate (SDS, ACS reagent
≥99.0%), and poly(ethylene glycol) (PEG) with molecular weights
of 400, 600, and 1500 g mol–1 were obtained from
Sigma Aldrich (Germany).
Sodium sulfate (Na2SO4, anhydrous, for analysis EMSURE ACS), magnesium sulfate heptahydrate
(MgSO4, for analysis EMSURE ACS), and PEG 1000 (EMPROVE
ESSENTIAL) were obtained from Merck Millipore (Germany).
Magnesium
chloride hexahydrate (MgCl2, 99.0–101.0%,
AnalaR NORMAPUR ACS Reag.) was purchased from VWR chemicals (Netherlands). n-Hexane (anhydrous, over molecular sieves) was acquired
from Alfa Aesar (Germany). 1,1,1-Tris(4-hydroxyphenyl)ethane (TPE,
>98.0%) was acquired from TCI (Belgium). PEG 200 was obtained from
Fluka (Germany).
Milli-Q water was used to prepare all aqueous
solutions. All chemicals
were used as received.
Hydrophilized polyethersulfone (PES)
ultrafiltration (UF) membranes
with a MWCO of 30 kDa were purchased from Microdyn-Nadir (UH030, Germany)
and used as the support. According to the supplier and earlier research,
these supports are stable in the pH range 0–14.11 (link)
8
Cultivation and Analysis of Microorganisms
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The MH agar was from BioMéroeux (Marcy-l’Étoile, France), the MH broth was from Oxoid (Hampshire, UK) and the Karmali agar was from Biolife (Milan, Italy). The glycerol solution was from Kemika (Zagreb, Croatia), the phosphate-buffered saline (PBS) was from Oxoid, and the kanamycin, dimethylsulphoxide (DMSO), resazurin, menadione, and Folin–Ciocalteu reagent were from Sigma Aldrich (Steinheim, Germany). The sodium chloride, magnesium sulfate heptahydrate, L-arginine, and 96% ethanol were from Merck (Darmstadt, Germany). The casamino acid was from Thermo Fisher Scientific (Carlsbad, CA, USA).
9
Characterization of Fibrous Magnesium Sulfate
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Two different mineral samples were tested in this work. The first sample (Epsom salt) is a synthetic phase of pure magnesium sulfate (magnesium sulfate heptahydrate, Merck) and has been tested compared with natural epsomite. The second sample (epsomite crystals, named MP) is a natural fibrous magnesium sulfate collected in the Perticara mine (Rimini province, Italy) and described and characterized by Giordani, Meli, et al., (2022) , Giordani et al., (2024) (link). In this sample, a significant fraction of the fibers shows a very small size, and it is potentially inhalable for humans. Moreover, MP sample's calculated Aerodynamic Equivalent Diameter (Dae) is 5.1 μm. Accordingly, the MP fibers can easily penetrate and be deposited in the laryngeal and bronchial respiratory tract (Giordani, Meli, et al., 2022) . The chemical composition of the MP sample is sulfur trioxides (32.93 wt%) and magnesium oxides (16.34 wt%); the water content, calculated by difference, is 50.73 wt %. It is worth noting that the MP sample contains a significant amount of radiogenic isotope 210 Po (about 5.5 Bq/g) and a minor amount of other trace elements ( 228 Th, Pb, As, Co, Fe, Mn, Ni, i, Sr, Ti, Zn) (Giordani et al., 2024; (link)Giordani, Meli, et al., 2022) .
10
Anaerobic E. coli K-12 Growth Assay
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The bacterial strain used in this study was E. coli K-12 BW25113 (Baba et al., 2006 (link)). For each biological replicate, a single colony of bacteria was used to inoculate a 10 ml starter culture in M63 minimal medium [(NH4)2SO4 2 g/L, KH2PO4 13.6 g/L, and FeSO4.7H2O 0.5 mg/L were mixed together, pH was adjusted to 7 with KOH, the solution was autoclaved and then 1 ml of 1 M sterile MgSO4.7H2O and sterile glucose to a final concentration of 0.4% were added before use] (Miller et al., 1992 ) incubated overnight at 37°C with shaking at 200 rpm. On the next day, the overnight starter cultures were diluted 1:100 to inoculate 58 ml of the same medium in 50 ml falcon centrifuge tubes and incubated at 37°C with shaking at 200 rpm until the OD600 reached 0.3. The falcon centrifuge tubes were filled to the top with 58 ml medium in order to create an anaerobic atmosphere as far as possible. Please note that some residual oxygen availability cannot be excluded as polypropylene tubes are potentially allowing some minimal oxygen diffusion into the sample (Karian, 2003 ). A total of 8 samples, 4 palladium-treated and 4 controls, were prepared. Ammonium sulfate, potassium phosphate, ferrous sulfate heptahydrate, potassium hydroxide, magnesium sulfate heptahydrate, and D(+)-Glucose were purchased from Merck Life Science AS, Oslo, Norway.
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