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Somnosuite system

Manufactured by Kent Scientific
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Sourced in United States

The SomnoSuite system is a laboratory equipment designed for physiological monitoring. It provides reliable data acquisition and analysis capabilities for researchers.

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Lab products found in correlation

Model 1900, by Kopf Instruments (1 mentions) Thermostatically controlled heating plate, by World Precision Instruments (1 mentions)

4 protocols using somnosuite system

1

Viral Gene Delivery to PVH in Obesity Model

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We used the SomnoSuite system (Kent Scientific, USA) to deliver isoflurane (2–5%) to anaesthetise mice before placing them in a stereotaxic frame (Model 1900; Kopf Instruments, USA). With a continuous flow of isoflurane (2%) in mouse nose mask, the mouse skull was exposed for intracranial injections. We injected AAV-Cre-GFP or AAV-GFP (~50 nl, , titre 8×1012 vg/ml; University of North Carolina Vector Core, USA) bilaterally into the paraventricular nucleus of the hypothalamus (PVH) of 7-week-old Mc4rloxP/loxP mice (coordinates from bregma: anteroposterior, ~0.70 mm; mediolateral, ~0.22 mm; dorsoventral, ~4.80 mm) using a 33 G Hamilton syringe attached to UltraMicroPump (UMP3–1; WPI, USA). Four weeks after the administration of the viral vectors, we measured glucose tolerance and urine glucose levels in these mice.
de Souza Cordeiro L.M., Elsheikh A., Devisetty N., Morgan D.A., Ebert S.N., Rahmouni K, & Chhabra K.H. (2020). Hypothalamic MC4R regulates glucose homeostasis through adrenaline-mediated control of glucose reabsorption via renal GLUT2 in mice. Diabetologia, 64(1), 181-194.
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2

Mice Electrophysiology and Muscle Contractility

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During electrophysiological and muscle contractility measurements, mice were anesthetized with inhaled isoflurane using the Kent Scientific SomnoSuite System (induction: 2.5%–3.5%, maintenance: 1%–2%) (Torrington, CT, USA). During electrophysiological and SFEMG recordings, animal body temperature was maintained at 37 °C using a thermostatically controlled heating plate (World Precision Instruments, Sarasota, FL, USA). During the muscle contractility measurements, a warm water bath HTP-1500 heat therapy pump (Adroit Medical Systems, Loudon, TN, USA) was set to maintain temperature of the testing stage at 37 °C. During all procedures performed under anesthesia, lubricating ointment was applied to the eyes to prevent corneal dryness (Dechra Veterinary Products, Overland Park, KS, USA). All measurements were performed on the right hindlimb which was shaved before the studies. All procedures that required anesthesia lasted for no more than 20 minutes.
Chugh D., Iyer C.C., Wang X., Bobbili P., Rich M.M, & Arnold W.D. (2019). Neuromuscular junction transmission failure is a late phenotype in aging mice. Neurobiology of aging, 86, 182-190.
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3

Stereotaxic Injection of C6 Glioma Cells

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The animals were anesthetized with isoflurane (2–4%) by SomnoSuite® system (Kent Scientific Corporation, Torrington, USA) and placed into the Neurostar Robot stereotaxic StereoDrive® instrument (NeuroStar GmbH, Tübingen, Germany), which holds the skull with ear bars and a clamp system that tightens against the frontonasal bone and the palate. After making a skin inscision on the dorsal region of the skull, the system was calibrated using the follow reference points: bregma; lambda and 2 mm right and left of central point between bregma and lambda. The target injection site was defined by the following stereotaxic coordinates: 2.0 mm (bregma; rostral-caudal axis), 2.0 mm (medial-lateral axis), and 3.0 mm (ventral-dorsal axis) of Paxinos Atlas [92 ] and 106 C6 cells suspended in 10µl of DMEM/F12 was injected in the 3,6 µL/min, using the Hamilton syringe of 10 µL (Hamilton Company, Nevada, USA). The bone recomposition was performed with Dencrilay® (Dencril Produtos Odontológicos, Brazil) diluted in self-curing acrylic based polymethyl methacrylate (Classic Dental Products, Brazil) and posterior suture of the animal.
Rego G.N., Nucci M.P., Mamani J.B., Oliveira F.A., Marti L.C., Filgueiras I.S., Ferreira J.M., Real C.C., Faria D.D., Espinha P.L., Fantacini D.M., Souza L.E., Covas D.T., Buchpiguel C.A, & Gamarra L.F. (2020). Therapeutic Efficiency of Multiple Applications of Magnetic Hyperthermia Technique in Glioblastoma Using Aminosilane Coated Iron Oxide Nanoparticles: In Vitro and In Vivo Study. International Journal of Molecular Sciences, 21(3), 958.
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4

Animal Housing and Anesthesia Protocol

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Male Wistar rats, and male and female C57BL6 and CD1 mice were housed under standard laboratory conditions. All interventions were carried out under 2.5% isoflurane anesthesia (SomnoSuite system, Kent Scientific, Torrington, CT). Experiments were approved by the Bioethics Committee of the Institute of Neurobiology of the National University of Mexico (UNAM) according to the US National Research Council’s Guide for the Care and Use of Laboratory Animals (Eighth Edition, National Academy Press, Washington, D.C., USA).
Robles J.P., Zamora M., Siqueiros-Marquez L., Adan-Castro E., Ramirez-Hernandez G., Nuñez F.F., Lopez-Casillas F., Millar R.P., Bertsch T., Martínez de la Escalera G., Triebel J, & Clapp C. (2021). The HGR motif is the antiangiogenic determinant of vasoinhibin: implications for a therapeutic orally active oligopeptide. Angiogenesis, 25(1), 57-70.
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