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Diazoxide

Manufactured by Cayman Chemical
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Diazoxide is a pharmaceutical compound used as a laboratory reagent. It functions as a potassium channel opener, affecting cellular ion transport mechanisms.

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Lab products found in correlation

2 n 7 nitrobenz 2 oxa 1 3 diazol 4 yl amino 2 deoxyglucose 2 nbdg, by Thermo Fisher Scientific (1 mentions) Alloxan monohydrate, by Merck Group (1 mentions) β cyclodextrin, by Tokyo Chemical Industry (1 mentions) Glimepiride, by Cayman Chemical (1 mentions) Tetraethyl ammonium tea, by Merck Group (1 mentions) Quinine, by Merck Group (1 mentions) Glibenclamide, by Merck Group (1 mentions) Ruthenium red, by Merck Group (1 mentions) Quinidine, by Merck Group (1 mentions) Tolbutamide, by Merck Group (1 mentions) 4 aminopyridine 4 ap, by Merck Group (1 mentions) Margatoxin, by Merck Group (1 mentions) Dopamine hcl, by Merck Group (1 mentions) Anti bax antibody, by Cell Signaling Technology (1 mentions) Streptozotocin (stz), by Merck Group (1 mentions) Hoechst 33258, by Merck Group (1 mentions) Anti p nf κb p65 antibody, by Cell Signaling Technology (1 mentions) Pyrrolidine dithiocarbamate, by Merck Group (1 mentions) Dmem medium, by Thermo Fisher Scientific (1 mentions) Pinacidil, by Cayman Chemical (1 mentions)

4 protocols using diazoxide

1

Fluorescent Glucose Uptake Analysis

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β-Cyclodextrin was purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan) and 20(S)-protonaxadiol 20-O-D-glucopyranoside (compound K) was obtained from Climax Biotech Co., Ltd. (Chengdu, China). Alloxan monohydrate and sea salts were obtained from Sigma Chemical Co. (St. Louis, MO, USA), and 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG) was purchased from Invitrogen Co., Ltd. (Grand Island, NY, USA). Glimepiride and diazoxide were purchased from Cayman Chemical Co. (Ann Arbor, MI, USA) and Santa Cruz Biotechnology Inc. (Dallas, TX, USA), respectively. Fluorescence microscopy was conducted using an Olympus 1X70 microscope (Olympus Co., Ltd., Tokyo, Japan). Focus Lite (Focus Co, Daejeon, Korea) and Image J (National Institutes of Health, Bethesda, MD, USA) software were used for image analysis.
Nam Y.H., Le H.T., Rodriguez I., Kim E.Y., Kim K., Jeong S.Y., Woo S.H., Lee Y.R., Castañeda R., Hong J., Ji M.G., Kim U.J., Hong B.N., Kim T.W, & Kang T.H. (2016). Enhanced antidiabetic efficacy and safety of compound K⁄β-cyclodextrin inclusion complex in zebrafish. Journal of Ginseng Research, 41(1), 103-112.
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2

Pharmacological Modulation of Ion Channels

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The KATP inhibitors glibenclamide and tolbutamide were purchased from Sigma and used at final concentrations of 10 μM and 200 μM unless stated otherwise. The K+ channel blockers tetraethylammonium (TEA), quinine, quinidine, 4-aminopyridine (4-AP), ruthenium red (RR), apamin, bupivacaine hydrochloride (BupHCl) and margatoxin were purchased from Sigma and used at the stated concentrations. The KATP channel activator diazoxide was purchased from Cayman Chemical and used at 50 μM unless stated otherwise. The MEK1/2 inhibitor U0126 (Calbiochem) was used at 20 μM. Staurosporine (Calbiochem) was used at a final concentration of 1 μM.
Scarth J.A., Wasson C.W., Patterson M.R., Evans D., Barba-Moreno D., Carden H., Cassidy R., Whitehouse A., Mankouri J., Samson A., Morgan E.L, & Macdonald A. (2023). Exploitation of ATP-sensitive potassium ion (KATP) channels by HPV promotes cervical cancer cell proliferation by contributing to MAPK/AP-1 signalling. Oncogene, 42(34), 2558-2577.
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3

Pharmacological Modulation of Cellular Pathways

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Drugs and chemicals were first dissolved in aqueous or dimethyl sulfoxide stock solutions before being added to the slice superfusate. Stock solutions were diluted at least 1:1000 in the superfusate immediately prior to use. Our previous experience showed that this dilute concentration of dimethyl sulfoxide had no effect on membrane conductance. Drugs delivered via superfusion passed through a heat exchanger and entered the slice chamber within 30 sec. In some experiments A769662 was added directly to the internal pipette solution and was delivered to the intracellular contents by passive dialysis. Dorsomorphin (Compound C) and 6,7-dihydro-4-hydroxy-3-(2′-hydroxy[1,1′-biphenyl]-4-yl)-6-oxo-thieno[2,3-b]pyridine-5-carbonitrile (A769662) were obtained from Tocris Cookson Inc. (Bristol, UK), whereas dopamine HCl was obtained from Sigma-Aldrich (St. Louis, MO, USA). Diazoxide was purchased from Cayman Chemical (Ann Arbor, MI, USA).
Wu Y.N., Shen K.Z, & Johnson S.W. (2017). Differential actions of AMP kinase on K-ATP currents in ventral tegmental area and substantia nigra zona compacta neurons. The European journal of neuroscience, 46(11), 2746-2753.
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4

Mitochondrial KATP Channel Modulation

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(NRG), 5,5′,6,6′-tetrachloro-1,1′,3,3′tetraethyl-imidacarbocyanine iodide (JC-1), Hoechst 33258, STZ and pyrrolidine dithiocarbamate (PDTC, a specific inhibitor of NF-κB pathway) were purchased from Sigma-Aldrich. Diazoxide (DZ, a mitochondrial K ATP channel opener), pinacidil (Pin, a non-selective K ATP channel opener), 5-hydroxydecanoic acid (5-HD, a mitochondrial K ATP channel blocker) and glibenclamide (Gli, a non-selective K ATP channel blocker) were purchased from Cayman Chemical. The cell counter kit-8 (CCK-8) was supplied by Dojindo Lab (Kumamoto, Japan). Fetal bovine serum (FBS) and DMEM medium were obtained from Gibco BRL; anti-kir6.1 (R-14) was purchased from Santa Cruz Biotechnology; anti-p-NF-κB p65 antibody, anti-p65 antibody anticaspase-3 antibody, anti-bax antibody and anti-bcl-2 antibody were purchased from Cell Signaling Technology; HRPconjugated secondary antibody and BCA protein assay kit were obtained from KangChen Bio-tech, Inc. (Shanghai, China). Enhanced chemiluminescence (ECL) solution was purchased from KeyGen Biotech (Nanjing, China).
You Q., Wu Z., Wu B., Liu C., Huang R., Yang L., Guo R., Wu K, & Chen J. (2016). Naringin protects cardiomyocytes against hyperglycemia-induced injuries in vitro and in vivo. The Journal of endocrinology, 230(2).
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