For in vitro activation, naïve CD4+ T-cells were cultured in R10 medium and stimulated with plate-bound anti-CD3 (0.2 μg/ml, 0.5 μg/ml, 1.0 μg/ml) (Clone 145-2C11, BioX Cell, West Lebanon, NH) and soluble anti-CD28 Abs (1.0 μg/ml) (Clone 37.51, BioX Cell, West Lebanon, NH) for 24 and 48 h. For Th1 cell polarization, cells were stimulated with plate-bound anti-CD3 (1.0 μg/ml) and soluble anti-CD28 Abs (1.0 μg/ml) in the presence of IL-12 (10 ng/ml, Biolegend) and anti-IL-4 Ab (10 μg/ml) (Clone 11B11, BioX Cell, West Lebanon, NH) for 72 h. For phospho-STAT4 detection, cells were activated with plate-bound anti-CD3 (1.0 μg/ml) and soluble anti-CD28 (1.0 μg/ml) for 24 h, then stimulated with IL-12 (10 ng/ml) for the indicated time points.
Anti il 4 ab
Manufactured by BioXCell
Anti-IL-4 Ab is a laboratory antibody product that binds to and neutralizes the cytokine Interleukin-4 (IL-4). It can be used in various research applications to study the role of IL-4 in biological processes.
Automatically generated - may contain errors
Lab products found in correlation
Anti cd3, by BioXCell (2 mentions)
Soluble anti cd28 abs, by BioXCell (2 mentions)
Soluble anti cd28 ab, by BioLegend (2 mentions)
Il 12, by BioXCell (2 mentions)
Il 12, by BioLegend (2 mentions)
Anti il 21 ab, by R&D Systems (1 mentions)
Nightowl 2, by Berthold Technologies (1 mentions)
Recombinant il 4, by Thermo Fisher Scientific (1 mentions)
Thioglycollate broth, by Merck Group (1 mentions)
Clone 11b11, by BioXCell (1 mentions)
Lipopolysaccharides (lps), by Merck Group (1 mentions)
4 protocols using anti il 4 ab
1
In Vitro Activation and Polarization of CD4+ T Cells
2
Tumor Models and Therapeutic Antibodies
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An MCA205 fibrosarcoma cell line expressing OVA protein was previously established21 (link). RMA cells, a Rauscher MuLV-induced lymphoma21 (link) was kindly provided by Dr Akira Shibuya (University of Tsukuba, Japan). Mice were inoculated subcutaneously with 8 × 105 MCA-OVA or 1 × 105 RMA, or were intravenously injected with 1 × 105 of the OVA/firefly luciferase-expressing melanoma cell line, MO4-Luc26 (link)52 (link), in the pulmonary metastatic model. Tumour size is expressed as a tumour index, the square root of (length × width), as described previously53 (link). To measure MO4 metastasis in the lung, luminescence images were analysed using NightOWL II (Berthold Technologies)26 (link). Two hundred μg of anti-IL-6R Ab, 15A7; anti-IL-6 Ab, MP5–20F3 (BioXCell); or control rat IgG Ab (Millipore) was injected 1 day before and after immunization. Daily intraperitoneal injections of 200 μg of anti-IL-4 Ab (BioXCell) and 100 μg of anti-IL-21 Ab (R&D Systems) were performed for 2 days after T-cell transfer. Two hundred μg of anti-IL-10 Ab (JES5-2A5; BioXCell) was injected every other day after tumour inoculation. For in vivo cell depletion, mice were injected with 200 μg of anti-CD4 Ab (GK1.5), anti-CD8 Ab (2.43) or anti-Gr-1 Ab (R56–86; BioXCell) 4–5 days before T-cell transfer, immunization or tumour inoculation.
3
Inflammatory Cell Recruitment Assay
4
In Vitro Activation and Polarization of CD4+ T Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For in vitro activation, naïve CD4+ T-cells were cultured in R10 medium and stimulated with plate-bound anti-CD3 (0.2 μg/ml, 0.5 μg/ml, 1.0 μg/ml) (Clone 145-2C11, BioX Cell, West Lebanon, NH) and soluble anti-CD28 Abs (1.0 μg/ml) (Clone 37.51, BioX Cell, West Lebanon, NH) for 24 and 48 h. For Th1 cell polarization, cells were stimulated with plate-bound anti-CD3 (1.0 μg/ml) and soluble anti-CD28 Abs (1.0 μg/ml) in the presence of IL-12 (10 ng/ml, Biolegend) and anti-IL-4 Ab (10 μg/ml) (Clone 11B11, BioX Cell, West Lebanon, NH) for 72 h. For phospho-STAT4 detection, cells were activated with plate-bound anti-CD3 (1.0 μg/ml) and soluble anti-CD28 (1.0 μg/ml) for 24 h, then stimulated with IL-12 (10 ng/ml) for the indicated time points.
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