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High glucose

Manufactured by Fujifilm
Sourced in Japan

The high glucose lab equipment product is a versatile tool designed for measuring and analyzing glucose levels in various samples. It provides accurate and reliable results, enabling efficient monitoring and control of glucose concentrations in a wide range of applications.

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3 protocols using high glucose

1

Culture of Immortalized POAG Cell Line

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Immortalized TM cells GTM3 (Novartis, Basel, Switzerland) established from POAG patient eyes were incubated in D-MEM (Dulbecco’s Modified Eagle’s Medium) (high glucose) (Wako, Osaka, Japan) supplemented with 10% fetal bovine serum (FCS) (Thermo Fisher Scientific, MA, USA) [17 (link)].
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2

RAW-blue Cell Culture Conditions

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RAW-blue cells were cultured in DMEM, High Glucose with L-glutamine, phenol red and sodium pyruvate (Wako Pure Chemical Industries, Ltd.) supplemented with 10% heat-inactivated FBS, 50 U/ml penicillin, 50 µg/ml streptomycin and 100 µg/ml normocin at 37˚C in a humidified incubator with 5% CO2.
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3

Oxaliplatin-resistant Gastric Cancer Cells

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The human gastric cancer cell line, OCUM-2M, and the oxaliplatin-resistant subcell line, OCUM-2M/OXA, were cultured in Dulbecco's modified Eagle's medium (DMEM) containing high glucose (Fujifilm Wako Pure Chemical Corporation, Osaka, Japan) and supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 0.1 mg/mL streptomycin (Meiji Seika Pharma Co., Tokyo, Japan) in a humidified 5% CO2 incubator at 37°C. The cell lines were derived from a diffuse-type human gastric cancer patient, and the subline was established from parental cells by stepwise exposure to oxaliplatin.(11 (link))
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