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Clone b 5 1 2

Manufactured by Santa Cruz Biotechnology
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Clone B-5–1-2 is a laboratory reagent used for research purposes. It is a cellular clone derived from a specific genetic line. The core function of this product is to serve as a research tool for studies involving its particular cellular characteristics and properties.

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Lab products found in correlation

Amersham ecl prime reagent, by GE Healthcare (2 mentions) Image lab v5, by Bio-Rad (2 mentions) Clone d7d7w, by Bio-Rad (2 mentions) Clone 7f9, by Merck Group (2 mentions) Cleaved caspase 3, by Cell Signaling Technology (2 mentions) Chemidoc xrs system, by Bio-Rad (2 mentions) Polyvinylidene difluoride membrane, by Bio-Rad (2 mentions)

Close spelling variants of this product

B-2 clone (2 citations)

2 protocols using clone b 5 1 2

1

Western Blot Analysis of NF-κB Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
20 μg of protein was separated by SDS-PAGE, transferred onto a polyvinylidene difluoride membrane (Bio-Rad), blocked with 5% non-fat milk and immunoblotted overnight at 4°C with antibodies against RelB (1:1000, Clone D7D7W, Cat. No. 10544, Cell Signaling), p52 (and p100) (1:1000, polyclonal, Cat. No. 4882, Cell Signaling), cleaved caspase-3 (1:1000, polyclonal, Cat. No. 9661, Cell Signaling) and β-catenin (1:1000, clone 14, Cat. No. 610154, BD Biosciences). Antibody binding was detected with HRP-conjugated secondary antibodies and developed using Amersham ECL Prime reagent (GE Healthcare). Bands were detected and quantified using the Chemidoc XRS+ system running ImageLab v5.2.1 software (Bio-Rad) or using photographic film and ImageJ (v1.49o), and normalized to β-actin levels (anti-β-actin-peroxidase conjugated mouse monoclonal antibody, 1:50000, clone AC-15, Cat. No. A3854, Sigma-Aldrich), vinculin (anti-vinculin, 1:1000, clone 7F9, Cat. No. sc-73614, Santa Cruz) or tubulin (anti-tubulin, 1:5000, clone B-5–1-2, Cat. No. T6074, Sigma-Aldrich).
Conlon T.M., John-Schuster G., Heide D., Pfister D., Lehmann M., Hu Y., Ertüz Z., Lopez M.A., Ansari M., Strunz M., Mayr C., Ciminieri C., Costa R., Kohlhepp M.S., Guillot A., Günes G., Jeridi A., Funk M.C., Beroshvili G., Prokosch S., Hetzer J., Verleden S.E., Alsafadi H., Lindner M., Burgstaller G., Becker L., Irmler M., Dudek M., Janzen J., Goffin E., Gosens R., Knolle P., Pirotte B., Stoeger T., Beckers J., Wagner D., Singh I., Theis F.J., de Angelis M.H., O’Connor T.O., Tacke F., Boutros M., Dejardin E., Eickelberg O., Schiller H.B., Königshoff M., Heikenwalder M, & Yildirim A.Ö. (2020). Inhibition of LTβR-signalling activates Wnt-induced regeneration in lung. Nature, 588(7836), 151-156.
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2

Western Blot Analysis of NF-κB Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
20 μg of protein was separated by SDS-PAGE, transferred onto a polyvinylidene difluoride membrane (Bio-Rad), blocked with 5% non-fat milk and immunoblotted overnight at 4°C with antibodies against RelB (1:1000, Clone D7D7W, Cat. No. 10544, Cell Signaling), p52 (and p100) (1:1000, polyclonal, Cat. No. 4882, Cell Signaling), cleaved caspase-3 (1:1000, polyclonal, Cat. No. 9661, Cell Signaling) and β-catenin (1:1000, clone 14, Cat. No. 610154, BD Biosciences). Antibody binding was detected with HRP-conjugated secondary antibodies and developed using Amersham ECL Prime reagent (GE Healthcare). Bands were detected and quantified using the Chemidoc XRS+ system running ImageLab v5.2.1 software (Bio-Rad) or using photographic film and ImageJ (v1.49o), and normalized to β-actin levels (anti-β-actin-peroxidase conjugated mouse monoclonal antibody, 1:50000, clone AC-15, Cat. No. A3854, Sigma-Aldrich), vinculin (anti-vinculin, 1:1000, clone 7F9, Cat. No. sc-73614, Santa Cruz) or tubulin (anti-tubulin, 1:5000, clone B-5–1-2, Cat. No. T6074, Sigma-Aldrich).
Conlon T.M., John-Schuster G., Heide D., Pfister D., Lehmann M., Hu Y., Ertüz Z., Lopez M.A., Ansari M., Strunz M., Mayr C., Ciminieri C., Costa R., Kohlhepp M.S., Guillot A., Günes G., Jeridi A., Funk M.C., Beroshvili G., Prokosch S., Hetzer J., Verleden S.E., Alsafadi H., Lindner M., Burgstaller G., Becker L., Irmler M., Dudek M., Janzen J., Goffin E., Gosens R., Knolle P., Pirotte B., Stoeger T., Beckers J., Wagner D., Singh I., Theis F.J., de Angelis M.H., O’Connor T.O., Tacke F., Boutros M., Dejardin E., Eickelberg O., Schiller H.B., Königshoff M., Heikenwalder M, & Yildirim A.Ö. (2020). Inhibition of LTβR-signalling activates Wnt-induced regeneration in lung. Nature, 588(7836), 151-156.
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