Anti shp 1 antibody

Manufactured by Abcam

Sourced in United States

Anti-SHP-1 antibody is a laboratory reagent used for the detection and quantification of the SHP-1 protein in various sample types. SHP-1 is a protein tyrosine phosphatase that plays a role in the regulation of cell signaling pathways.

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Lab products found in correlation

Control igg, by Santa Cruz Biotechnology (1 mentions) Protein a g plus agarose bead, by Santa Cruz Biotechnology (1 mentions) Ripa lysis buffer, by Beyotime (1 mentions) Rediplate 96 enzchekr tyrosine phosphatase assay kit, by Thermo Fisher Scientific (1 mentions) Protein g agarose beads, by Roche (1 mentions)

2 protocols using anti shp 1 antibody

Immunoprecipitation and Western Blotting

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Cell lysates were homogenized using RIPA lysis buffer (Beyotime Biotechnology, China) then incubated with or without anti-SMURF1 antibody (Santa Cruz Biotech., Santa Cruz, CA, USA; sc-100616), anti-SHP-1 antibody (Abcam; ab227503), or control IgG (Santa Cruz Biotech.; sc-2027) overnight at 4 °C followed by an incubation with protein A/G Plus agarose beads (Santa Cruz Biotech.; sc-2003) for 2 h at 4 °C. The immunoprecipitated complexes were washed three times in lysis buffer and analyzed by Western blotting. The membrane was then incubated with the secondary antibody, IPKine HRP, mouse anti-rabbit IgG LCS (Abbkine, California, CA, USA; A25022), to eliminate heavy chain interference.

Bian Y., Yuan L., Yang X., Weng L., Zhang Y., Bai H, & Chen J. (2021). SMURF1-mediated ubiquitylation of SHP-1 promotes cell proliferation and invasion of endometrial stromal cells in endometriosis. Annals of Translational Medicine, 9(5), 362.

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Measuring SHP-1 Phosphatase Activity

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The phosphatase activity of SHP-1 was detected as previously reported 25 (link). In brief, SHP-1 protein in cell lysates was incubated with anti-SHP-1 antibody (Cat. ab227503, Abcam, Cambridge, MA) at 4°C overnight and precipitated with Protein G-Agarose beads (Roche). A RediPlate 96 EnzChekR Tyrosine Phosphatase Assay Kit (Cat. R-22067) was used for the SHP-1 activity assay (Molecular Probes, Invitrogen).

Liu X., Chen Z., Li S., Jin L., Cui X., Cui C., Deng Y., Gao Q., Fan L., Niu Y., Wang W., Cui C., Zhong J., Cui Q., Geng B, & Cai J. (2022). Pre-miRNA Hsa-Let-7a-2: a Novel Intracellular Partner of Angiotensin II Type 2 Receptor Negatively Regulating its Signals. International Journal of Biological Sciences, 18(8), 3237-3250.

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