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Fluorinert fc 77

Manufactured by 3M
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Fluorinert FC-77 is a perfluorinated fluid manufactured by 3M. It is a clear, colorless, and odorless liquid with a high density and low surface tension. Fluorinert FC-77 is primarily used as a coolant and heat transfer medium in various industrial and laboratory applications due to its excellent thermal and chemical stability.

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Lab products found in correlation

Micro2.5 gradient system, by Bruker (1 mentions) Micro ct, by GE Healthcare (1 mentions) Vivoquant, by Invicro (1 mentions) Avance 3 nmr spectrometer, by Bruker (1 mentions)

4 protocols using fluorinert fc 77

1

MRI Imaging of Tissue Samples

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The tissue samples were removed from the storing solution and cut to a suitable sample size. The specimens were immersed into a 20-mm-NMR-tube with a perfluorinated fluid (Fluorinert™ FC-77, 3M Belgium NV/SA, Zwijndrecht, Belgium) which prevents dehydration and contributes no proton signal during MRI measurements.
MR imaging was performed using a 7-T Bruker Avance nonclinical NMR spectrometer with a vertical-bore magnet (300 MHz Larmor frequency for protons, Bruker BioSpin, Rheinstetten, Germany) using a linear polarized birdcage radiofrequency coil of 20 mm inner diameter and a Bruker Micro 2.5 gradient system generating a maximum magnetic field gradient strength of up to 1 T/m on three axes. The experimental parameters used for imaging are summarized in Table 2.
Elschner C., Korn P., Hauptstock M., Schulz M.C., Range U., Jünger D, & Scheler U. (2017). Assessing agreement between preclinical magnetic resonance imaging and histology: An evaluation of their image qualities and quantitative results. PLoS ONE, 12(6), e0179249.
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2

High-Resolution Brain Imaging in Mice

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The brain MRI and microCT vascular atlas was analyzed from ex vivo data acquired by Dorr et al. [39 (link)] in male CBA mice. For MRI, a 7.0-T MRI scanner (Varian Inc., Palo Alto, CA, USA) was used with skulls placed into proton-free susceptibility-matching fluid (Fluorinert FC-77, 3M Corp., St. Paul, MN, USA). The parameters used in the scans were optimized for grey/white matter contrast: T2W, 3D fast spin-echo sequence, with TR/TE = 325/32 ms, four averages, field-of-view 12 × 12 × 25 mm3 and matrix size = 780 × 432 × 432 resulting in an image with 32 μm isotropic voxels. For microCT (GE Healthcare, Chicago, IL, USA) the brains were removed from the skulls and mounted in 1% agar. Each vascular image volume was acquired with 20 μm isotropic resolution using the GE eXplore Locus SP specimen scanner. Images were obtained from 720 views over a 360° rotation in 2 h with an X-ray tube current of 80 μA and voltage of 80 kVp.
Taylor E.N., Huang N., Wisco J., Wang Y., Morgan K.G, & Hamilton J.A. (2020). The brains of aged mice are characterized by altered tissue diffusion properties and cerebral microbleeds. Journal of Translational Medicine, 18, 277.
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3

High-Resolution MRI of Fixed Brain Samples

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High-resolution MRI was performed on fixed brain samples with a compact MRI system (M2 permanent magnet system, 1.05 tesla, Aspect Imaging, Shoham, Israel). Fixed samples were transferred to a modified disposable 10 mL syringe filled with a proton-free susceptibility-matching fluid (Fluorinert FC-77, 3M Company, Saint Paul, MN, USA) and scanned. T1-weighted FLASH-3D sequences were performed under the following parameters: TE (echo time)/TR (repetition time) = 12.0 ms/50.0 ms for about 37 min per sample, 0.10 × 0.10 × 0.48 mm voxel. T2-weighted RARE sequences were performed under the following parameters: TE/TR = 95.0 ms/5484.5 ms for about 8.5 min per sample, 0.15 × 0.15 mm pixel, 1.25 mm slice + 0.25 mm gap. Image processing of the digital 3D MRI data was performed using VivoQuant (inviCRO, Boston, MA, USA), a sophisticated image processing and analysis software package that is fully integrated into the M2 imaging system.
Taketa Y., Shiotani M., Tsuru Y., Kotani S., Osada Y., Fukushima T., Inomata A, & Hosokawa S. (2015). Application of a compact magnetic resonance imaging system for toxicologic pathology: evaluation of lithium-pilocarpine-induced rat brain lesions. Journal of Toxicologic Pathology, 28(4), 217-224.
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4

Multiexponential T2 Relaxation Analysis

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MR data were acquired at 4°C with a 5-mm solenoid coil using a 9.4T/105mm Bruker Avance III NMR spectrometer (Bruker Biospin GmbH, Rheinstetten, Germany). Samples were placed in a 5-mm diameter glass tube filled with Fluorinert FC-77 (3M Corp., St. Paul, MN), a proton-free susceptibility matching fluid, and sealed with a Teflon lid. Teflon screws were used to accurately position each sample along the length of the tube at the center of the RF coil. Relaxation data were acquired using a non-localized Carr Purcell Meiboom Gill (CPMG) sequence with echo time TE = 0.09 ms, interpulse delay TR = 10s, 8192 echoes and 64 signal averages. Even echoes were used, resulting in an effective echo time of 0.18 ms. The total time for the acquisition of each transverse decay curve used for multiexponential analysis was ~12 minutes. Intensities were fit to a three-parameter monoexponential function to obtain conventional T2 relaxation times, and the same data were used for multiexponential T2 analysis.
Irrechukwu O.N., Von Thaer S., Frank E.H., Lin P.C., Reiter D.A., Grodzinsky A.J, & Spencer R.G. (2014). Prediction of Cartilage Compressive Modulus using Multiexponential Analysis of T2 Relaxation Data and Support Vector Regression. NMR in biomedicine, 27(4), 468-477.
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