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Goat α mouse igg hrp

Manufactured by Santa Cruz Biotechnology

Goat α-mouse IgG-HRP is a secondary antibody conjugated with horseradish peroxidase (HRP). It is designed to detect and bind to mouse immunoglobulin G (IgG) antibodies in various immunoassay applications.

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2 protocols using goat α mouse igg hrp

1

Western Blot Protein Detection

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Extracted protein was probed with anti-ALG-1 (1:5000, Pierce, PA1-031) and anti-β-tubulin (1:5000, Sigma-Aldrich, T4026) primary antibodies followed by goat α-rabbit IgG-HRP (1:5000, Santa Cruz Biotechnology, sc-2004) and goat α-mouse IgG-HRP (1:5000, Santa Cruz Biotechnology, sc-2031) secondary antibodies.
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2

Western Blot Analysis of MLKL and FKBP12

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Cell lysates were harvested and quantitated using a BCA Protein Assay (Thermo). 30μg total protein/sample were separated using SDS-PAGE gels (Invitrogen) and detected using traditional protocols. The following antibodies were used for protein detection: rat α-MLKL clone 3H1 (EMD Millipore), rabbit α-FKBP12 (Thermo), mouse α-actin C4 (EMD Millipore), goat α-rat IgG-HRP (Santa Cruz), donkey α-rabbit IgG-HRP (Santa Cruz), and goat α-mouse IgG-HRP (Santa Cruz).
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