2-AP, glutamate, and MK-801 were purchased from Sigma (St. Louis, MO, USA). Formaldehyde 37% (Vetec, Brazil), morphine hydrochloride (Vetec, Brazil), caffeine (Merck, USA), and naloxone hydrochloride (Cristália, Brazil) were also purchased and used in this study. All drugs were diluted in distilled water except 2-AP which required Tween 80 and distilled water. The other solvents and chemicals used in this study were purchased from Sigma-Aldrich® (São Paulo, São Paulo, Brazil).
Formaldehyde 37
Manufactured by Vetec
Sourced in Brazil
Formaldehyde 37% is a laboratory reagent used for various applications. It is a colorless, pungent-smelling liquid that is soluble in water, alcohol, and other organic solvents. Formaldehyde 37% is commonly used as a fixative, preservative, and disinfectant in various scientific and medical settings.
Automatically generated - may contain errors
Lab products found in correlation
Naloxone hydrochloride, by Cristália (2 mentions)
Diazepam, by Merck Group (2 mentions)
Morphine hydrochloride, by Merck Group (2 mentions)
Caffeine, by Merck Group (1 mentions)
Glutamate, by Merck Group (1 mentions)
Mk 801, by Merck Group (1 mentions)
Naloxonazine, by Merck Group (1 mentions)
5 fluorouracil, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Mallinckrodt (1 mentions)
Phenytoin, by Merck Group (1 mentions)
Tween 80, by Merck Group (1 mentions)
Glacial acetic acid, by Vetec (1 mentions)
Morphine, by Merck Group (1 mentions)
Tween 80, by Vetec (1 mentions)
Cytochalasin b, by Merck Group (1 mentions)
5 protocols using formaldehyde 37
1
Behavioral Pharmacology Protocol
2
Pharmacological Agents in Research
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The following drugs and reagents were acquired in Sigma-Aldrich® (St. Louis, MO, United States): 5-fluorouracil (5-FU), diazepam, naloxonazine, cyclophosphamide, and Tween 80. The anesthetics ketamine and xylazine were obtained from Konig S.A. (Brazil), and MSD Saúde Animal (Brazil), respectively. Other drugs, reagents, and kits comprise: heparin (HEPAMAXS®), ELISA Kit (eBioscience®, Inc. Science Center Drive, San Diego, CA, United States), kits for hematological and biochemical analysis (LABTEST; Lagoa Santa, MG, Brazil), morphine hydrochloride (Merck®), naloxone hydrochloride (Cristália®), formaldehyde 37% (Vetec), dimethylsulfoxide - DMSO (Mallinckrodt Chemicals - Phillipsburg, NJ, United States). The acridine derivative N’-(6-chloro-2-methoxy-acridin-9-yl)-2-cyanoacetohydrazide (ACS-AZ) was synthesized as previously described (Silva et al., 2018 (link)).
3
Intraperitoneal Drug Administration Protocol
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OXL, diazepam and phenytoin were purchased from Sigma (St. Louis, MO, USA). Glacial acetic acid and formaldehyde 37% were purchased from Vetec (São Paulo, Brazil). Morphine and Tween 80 were purchased from Merck (Darmstadt, Germany). All administrations were via intraperitoneal (i.p.) in volumes of 0.1 mL/10 g body weight.
4
Multidisciplinary Research Protocol
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Glacial acetic acid (Reagen - Brasil); Morphine hydrochloride (Merck – E. U. A.); Ethanol (IPeFarM/UFPB – Brasil); Formaldehyde 37% (Vetec – Brasil); RMD86 (UEPB - Brasil); Tween 80 (Vetec – Brasil); Diazepam (Cristália – Brasil); Acetaminophen (Medley - Brasil); Yeast (Saccharomyces cerevisiae, Saf do Brasil Produtos Alimentícios Ltd, Brazil).
5
Genotoxicity Assay of DFMC in Sarcoma Cells
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Sarcoma 180 cells were plated in 24-well plates (0.5 × 106 cells/mL) and treated with DFMC at 5, 25, and 50 μg/mL. After 44 h at 37°C, cytochalasin B (Sigma Aldrich, USA, 6 μg/mL) was added, and the cells were reincubated for an additional 28 h. At 72 h, the cultures were transferred to tubes and centrifuged at 800 rpm for 5 minutes. Then, the supernatant was removed, and the body of the cell bottom was enlarged and centrifuged again before the addition of 2 mL of fixing solution (methanol and acetic acid, ratio 5 : 1) and 3 drops of formaldehyde 37% (Vetec™, Brazil). This procedure was repeated 3x using fixing solution 3 : 1 without formaldehyde. Supernatants were discarded, and 2-3 drops of cell suspension were dripped onto slides and stained with Giemsa for 5 min [25 (link)]. Considering blind examination, a total of 2000 cells by concentration were counted by optical microscopy at 1000x (1000 cells/slide) to count buds, bridges, and micronuclei.
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