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2 protocols using quercetin luteolin

1

Characterization of Bee Bread Bioactives

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Bee bread was purchased in Iasi County, Romania. The sample was harvested in the spring of 2020. The sample was stored at −20 °C prior to any analysis. The bee bread was defatted using n-hexane (the oil was used for fatty acids characterization, while the defatted bee bread was used for bioactive compounds analysis). Methanol, Folin-Ciocalteau reagent, ethanol, AlCl3, sodium carbonate, ethyl ether, rosmarinic acid, p-coumaric acid, chlorogenic acid, vanillic acid, caffeic acid, p-hydroxybenzoic acid, protocatechiuc acid, gallic acid, kaempferol, quercetin luteolin and myricetin, fructose, glucose, sucrose, melesitose and raffinose were purchased from Sigma–Aldrich (Taufkirchen, Germany). FAMEs mix was purchased from Restek (Lisses, France).
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2

Antioxidant Capacity Evaluation Protocol

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Ethanol, mEthanol, glacial acetic acid, sodium acetate, acetonitrile, caffeic acid, ferulic acid, gallic acid, rosmarinic acid, p-coumaric acid, rutin, quercetin luteolin, apigenin, 2,2-diphenyl-1-picrylhydrazyl (DPPH), (±) -6-Hydroxy-2,5,7,8tetramethylchromane-2-carboxylic acid (Trolox), ethyl benzothiazoline-6-sulfonic acid diammonium 2,2'-azino-bis (ABTS), sodium chloride, aluminun chloride, sodium acetate, 7-Hydroxy-3Hphenoxazin-3-one-10-oxide sodium salt (Resazurin sodium salt), sodium carbonate, potassium persulfate and butylhydroxytoluene (BHT) were all purchased from Sigma-Aldrich, São Paulo -SP, Brazil.
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